公开(公告)号：US20230347312A1

公开(公告)日：2023-11-02

申请号：US18131159

申请日：2023-04-05

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Siyuan XING ,  Denis PRISTINSKI

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2204/00 ,  B01J2219/00416 ,  B01J2219/00596 ,  B01J2219/00632 ,  B01J2219/00722 ,  B01J2219/00831 ,  B01J2219/1233

Abstract: Systems and methods for dispensing a liquid on a substrate are disclosed. The system may be used to coat a substrate and/or a sample (e.g., a biological sample) with a liquid. The system includes a rotating device configured to rotate about an axis and a substrate disposed on the rotating device. The substrate comprises a well configured to hold a liquid and the substrate is positioned on a surface of the rotating device perpendicular to and concentric with the axis. The system further includes a liquid handler configured to dispense the liquid into the well. The rotating device is configured to rotate at a speed sufficient to disperse the liquid within the well.

公开(公告)号：US20230278028A1

公开(公告)日：2023-09-07

申请号：US18170413

申请日：2023-02-16

Applicant: 10x Genomics, Inc.

Inventor： Siyuan XING ,  Pratomo Putra Alimsijah ,  Denis Pristinski ,  David Morgan ,  Rajiv Bharadwaj ,  Augusto Manuel Tentori ,  Francis Marcogliese ,  Yiran Zhang

IPC: B01L3/00 ,  G01N21/05

CPC classification number: B01L3/502707 ,  B01L3/502715 ,  G01N21/05 ,  G01N2021/058

Abstract: Flowcell devices configured for reversible assembly, and methods of assembly, disassembly, and use thereof are provided. The methods and devices allow the interior of the flowcell device to be accessed without damaging, or otherwise disturbing sensitive samples and surfaces.

公开(公告)号：US20240060127A1

公开(公告)日：2024-02-22

申请号：US18343108

申请日：2023-06-28

Applicant: 10x Genomics, Inc.

Inventor： Denis PRISTINSKI ,  Preyas SHAH ,  Siyuan XING

IPC: C12Q1/6874 ,  C12Q1/6876 ,  G03F7/00

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  G03F7/0035

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate using a minimal mask scheme. Systems and kits employing the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photomasks and photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

公开(公告)号：US20230312209A1

公开(公告)日：2023-10-05

申请号：US18131223

申请日：2023-04-05

Applicant: 10x Genomics, Inc.

Inventor： David MORGAN ,  Evan DEJARNETTE ,  Eric EVJE ,  Yiran ZHANG ,  Siyuan XING

IPC: B65D75/36

CPC classification number: B65D75/36 ,  B65D2575/3218

Abstract: Systems that include blister packs and methods of use thereof are disclosed. In various embodiments, a system includes a blister pack having at least one blister comprising a base and a top layer. The blister contains a liquid reagent. The system further includes an actuator configured to release the liquid reagent from the blister. The system further includes a substrate configured to hold a tissue sample and receive the liquid reagent from the blister upon release.

公开(公告)号：US20230184663A1

公开(公告)日：2023-06-15

申请号：US18066104

申请日：2022-12-14

Applicant: 10x Genomics, Inc.

Inventor： Siyuan XING ,  William STANISLAUS ,  Yiran ZHANG

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1404 ,  B01L3/502723 ,  B01L2300/0663 ,  B01L2300/0654 ,  B01L2400/0622 ,  B01L2200/0684 ,  B01L2200/16 ,  G01N2015/1068

Abstract: Devices, systems, and methods for reducing the introduction of bubbles to flow cells and removing existing bubbles from flow cells are provided. The devices, systems, and methods detect and redirect bubbles away from the flow cell before the bubble reaches the inlet or wash away bubbles from within the flow cell.

公开(公告)号：US20230167489A1

公开(公告)日：2023-06-01

申请号：US18070887

申请日：2022-11-29

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Siyuan XING ,  Rajiv BHARADWAJ

IPC: C12Q1/6841 ,  C12Q1/6837

CPC classification number: C12Q1/6841 ,  C12Q1/6837

Abstract: The present invention features devices and methods for supplying a biological sample with a fluid. In various embodiments, a device includes a first layer and a second layer. The first layer includes a window having a thickness of about 1 μm to about 1000 μm. The first and second layers reversibly seal to form a flow path having an inlet and an outlet and bounded in part by the window.

公开(公告)号：US20220228210A1

公开(公告)日：2022-07-21

申请号：US17565047

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Denis PRISTINSKI ,  Preyas SHAH ,  Steven William SHORT ,  Dieter WILK ,  Siyuan XING

IPC: C12Q1/6874 ,  C12N15/10

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.