公开(公告)号：US20150068901A1

公开(公告)日：2015-03-12

申请号：US14482803

申请日：2014-09-10

Applicant: Agilent Technologies, Inc.

Inventor： John Mannion ,  Bo Curry ,  Brian Jon Peter

IPC: G01N27/447

CPC classification number: G01N27/4145 ,  G01N27/4473

Abstract: This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described.

Abstract translation: 本公开尤其提供了一种纳米流体装置感测装置。 在某些实施例中，该装置包括：a）包括地板和天花板的通道，b）沿通道的纵向轴线布置的通道的地板和/或天花板中的一组电荷传感器; c）在通道的入口端的通道的地板和/或天花板中的捕获区域; 以及d）第一电极和第二电极，其中所述第一和第二电极被定位成沿着所述通道的纵向轴线提供电泳力。 还描述了其它实施例，例如方法。

公开(公告)号：US09546398B2

公开(公告)日：2017-01-17

申请号：US14538632

申请日：2014-11-11

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  John Mannion ,  Joel Myerson

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q2525/125 ,  C12Q2525/186 ,  C12Q2537/157

Abstract: A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3′ end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3′ end.

Abstract translation: 提供了一种核酸测序方法。 在某些实施方案中，该方法包括获得包含核酸和引物的双链体，其中引物具有核酸酶抗性3'末端，将双链体与链终止子核苷酸和校对阅读聚合酶组合以产生其中聚合酶 在添加的链终止子核苷酸上空转，鉴定添加到引物末端的链终止子核苷酸; 并在聚合酶空转之后向引物的末端添加核酸酶抗性核苷酸并除去加入的链终止子核苷酸，从而产生包含模板的双链体和具有核酸酶抗性3'末端的延伸引物。

公开(公告)号：US20150132756A1

公开(公告)日：2015-05-14

申请号：US14538632

申请日：2014-11-11

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  John Mannion ,  Joel Myerson

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q2525/125 ,  C12Q2525/186 ,  C12Q2537/157

Abstract: A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3′ end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3′ end.

Abstract translation: 提供了对核酸进行测序的方法。 在某些实施方案中，该方法包括获得包含核酸和引物的双链体，其中引物具有核酸酶抗性3'末端，将双链体与链终止子核苷酸和校对阅读聚合酶组合以产生其中聚合酶 在添加的链终止子核苷酸上空转，鉴定添加到引物末端的链终止子核苷酸; 并在聚合酶空转之后向引物的末端添加核酸酶抗性核苷酸并除去加入的链终止子核苷酸，从而产生包含模板的双链体和具有核酸酶抗性3'末端的延伸引物。

公开(公告)号：US09683958B2

公开(公告)日：2017-06-20

申请号：US14482803

申请日：2014-09-10

Applicant: Agilent Technologies, Inc.

Inventor： John Mannion ,  Bo Curry ,  Brian Jon Peter

IPC: G01N27/414 ,  G01N27/447

CPC classification number: G01N27/4145 ,  G01N27/4473

Abstract: This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described.

公开(公告)号：US20140373606A1

公开(公告)日：2014-12-25

申请号：US14376430

申请日：2012-02-03

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Karsten Kraiczek ,  John Mannion ,  Kevin P. Killeen

IPC: G01N21/05 ,  B32B37/18 ,  B32B38/10 ,  G01N30/74

CPC classification number: G01N21/05 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0838 ,  B32B37/18 ,  B32B38/10 ,  B32B2551/00 ,  G01N30/74 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2021/052 ,  G01N2021/056 ,  G01N2030/027 ,  G01N2030/746 ,  G01N2201/08 ,  Y10T156/10

Abstract: A micromachined flow cell (100), comprising a substrate (102), and a freestanding tube (104) delimiting a fluidic conduit (800) therein and being integrally formed from material of the substrate (102).

Abstract translation: 一种微加工流动池（100），包括基底（102）和在其中限定流体导管（800）并由基底（102）的材料一体形成的独立管（104）。