公开(公告)号：US20230020217A1

公开(公告)日：2023-01-19

申请号：US17934268

申请日：2022-09-22

Applicant: AJINOMOTO CO., INC.

Inventor： Yu SUZUKI ,  Manabu KITAZAWA ,  Shimpei OGAWA

IPC: C12N5/074

Abstract: A cell proliferation rate can be improved by a medium for cell culture, containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) at a concentration of not less than 30 mM, and having an osmotic pressure of 220 to 340 mOsm/kg, and a cell culture method using the medium.

公开(公告)号：US20220348884A1

公开(公告)日：2022-11-03

申请号：US17863832

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Yusuke OHYA ,  Megumi NISHIYAMA ,  Atsushi KONISHI

IPC: C12N5/074

Abstract: A medium with a reduced osmotic pressure containing a basal medium, in which the osmotic pressure is adjusted to 150 to 250 mOsm/kg, is useful for culturing cells.

公开(公告)号：US20220073882A1

公开(公告)日：2022-03-10

申请号：US17455763

申请日：2021-11-19

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Mayumi TOKUYAMA

IPC: C12N5/077

Abstract: Culturing a cartilage or bone progenitor cell in a medium containing a TGF-β signal inhibitor and FGF is effective for expansion culture of a cartilage or bone progenitor cell, and inducing differentiation of a cartilage or bone progenitor cell obtained by such a method is effective for producing a chondrocyte or osteocyte.

公开(公告)号：US20230012723A1

公开(公告)日：2023-01-19

申请号：US17863646

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Megumi NISHIYAMA ,  Kotoe KOSEKI

IPC: C12N5/00 ,  C12N5/074

Abstract: Cells may be cultured by a method including the following steps: a first step of preparing a population of cell aggregates having a major axis of not more than 400 μm, and a second step of suspension culturing the population of cell aggregates obtained in the first step.

公开(公告)号：US20220348883A1

公开(公告)日：2022-11-03

申请号：US17863785

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Megumi NISHIYAMA

IPC: C12N5/074 ,  C12N5/0735

Abstract: A method including adding choline at a specific concentration to a medium is useful for culturing cells.

公开(公告)号：US20250026799A1

公开(公告)日：2025-01-23

申请号：US18914383

申请日：2024-10-14

Applicant: AJINOMOTO CO., INC.

Inventor： Naoko TSUYOSHI ,  Teruhisa MANNEN ,  Yoshihiko MATSUDA ,  Shimpei OGAWA ,  Yasumichi KOSEKI ,  Tomomi KADO

IPC: C07K14/50 ,  C12N5/077

Abstract: A method of producing cultured meat is provided. Cultured meat is produced by culturing animal cells in the presence of bovine basic fibroblast growth factor (bbFGF) produced by using a coryneform bacterium as an expression host.

公开(公告)号：US20230002729A1

公开(公告)日：2023-01-05

申请号：US17863546

申请日：2022-07-13

Applicant: AJINOMOTO CO., INC.

Inventor： Shimpei OGAWA ,  Takuya HIGUCHI ,  Shumpei FUROMITSU ,  Kenichiro ITO ,  Megumi NISHIYAMA

IPC: C12N5/0735

Abstract: A medium composition, containing a basic fibroblast growth factor (bFGF) at not less than 150 ng/mL is useful for culturing cells.

公开(公告)号：US20210284971A1

公开(公告)日：2021-09-16

申请号：US17213839

申请日：2021-03-26

Applicant: AJINOMOTO CO., INC.

Inventor： Takuya MATSUMOTO ,  Shimpei OGAWA ,  Shumpei FUROMITSU ,  Yusuke OHYA ,  Kotoe KOSEKI ,  Takuya HIGUCHI ,  Megumi NISHIYAMA

IPC: C12N5/074 ,  C12N5/0735

Abstract: By using an additive for culturing animal cells that contains an amino acid or glucose, or an amino acid and glucose; or a medium for culturing animal cells that contains an amino acid or glucose, or an amino acid and glucose in addition to a medium component; or a method for culturing animal cells that includes culturing the animal cells using the aforementioned medium, or adding the aforementioned additive to a culture, the proliferation ability of animal cells can be improved particularly when the animal cells are cultured using a serum-free medium or a low albumin medium.

公开(公告)号：US20170101372A1

公开(公告)日：2017-04-13

申请号：US15389639

申请日：2016-12-23

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshiro KITAHARA ,  Koji OHSUMI ,  Seiji KITAJIMA ,  Shimpei OGAWA

IPC: C07C335/20 ,  C07C335/16

CPC classification number: A61K31/17 ,  A61K31/277 ,  C07C335/16 ,  C07C335/20

Abstract: Sweetness receptor antagonists represented by formula (I): wherein each symbol is described herein, are useful for the prophylaxis or treatment of metabolic syndrome, diabetes, obesity and the like.