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公开(公告)号:US20210302334A1
公开(公告)日:2021-09-30
申请号:US17220357
申请日:2021-04-01
Inventor: Alexandra ROS , Daihyun KIM , Austin ECHELMEIER , Jorvani CRUZ VILLARREAL , Ana EGATZ-GOMEZ , Sebastian QUINTANA
IPC: G01N23/20025 , B33Y80/00 , B01L3/02 , B01L3/00
Abstract: Methods and systems are provided for serial femtosecond crystallography for reducing the vast amount of waste of injected crystals practiced with traditional continuous flow injections. A micrometer-scale 3-D printed water-in-oil droplet generator device includes an oil phase inlet channel, an aqueous phase inlet channel, a droplet flow outlet channel, and two embedded non-contact electrodes. The inlet and outlet channels are connected internally at a junction. The electrodes comprise gallium metal injected within the 3-D printed device. Voltage across the electrodes generates water-in-oil droplets, determines a rate for a series of droplets, or triggers a phase shift in the droplets. An external trigger generates the droplets based on the frequency of an XFEL utilized in droplet detection, thereby synchronizing a series of droplets with x-ray pulses for efficient crystal detection. The generated droplets can be coupled to an SFX with XFEL experiment compatible with common liquid injector such as a GDVN.
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2.
公开(公告)号:US20170297024A1
公开(公告)日:2017-10-19
申请号:US15510126
申请日:2015-09-17
Inventor: Alexandra ROS , Bahige G ABDALLAH
CPC classification number: B01L3/502761 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L2200/0652 , B01L2300/0654 , B01L2300/0816 , B01L2300/0848 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/0896 , B01L2300/123 , B01L2300/16 , B01L2400/0424 , B01L2400/0487 , B01L2400/049 , B01L2400/0655 , G01N1/38
Abstract: A microfluidic apparatus, systems and methods for microfluidic crystallization based on gradient mixing. In one embodiment, the apparatus includes (a) a first layer, (b) a plurality of first channels and a plurality of vacuum chambers both arranged in the first layer, where the plurality of vacuum chambers are each coupled to at least one of the first channels, (c) a membrane having first and second surfaces, where the first surface of the membrane is coupled to the first layer, (d) a second layer coupled to the second surface of the membrane, (e) a plurality of wells and a plurality of second channels both arranged in the second layer, where the wells are each coupled to at least one of the plurality of second channels and (f) a plurality of barrier walls each disposed in the plurality of second channels and arranged opposite to one of the plurality of vacuum chambers.
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3.
公开(公告)号:US20240068965A1
公开(公告)日:2024-02-29
申请号:US18504091
申请日:2023-11-07
Inventor: Alexandra ROS , Daihyun KIM , Austin ECHELMEIER , Jorvani CRUZ VILLARREAL , Ana EGATZ-GOMEZ , Sebastian QUINTANA
IPC: G01N23/20025 , B01L3/00 , B01L3/02 , B33Y80/00
CPC classification number: G01N23/20025 , B01L3/0241 , B01L3/50273 , B33Y80/00 , B01L2300/0867 , G01N2223/602
Abstract: Methods and systems are provided for serial femtosecond crystallography for reducing the vast amount of waste of injected crystals practiced with traditional continuous flow injections. A micrometer-scale 3-D printed water-in-oil droplet generator device includes an oil phase inlet channel, an aqueous phase inlet channel, a droplet flow outlet channel, and two embedded non-contact electrodes. The inlet and outlet channels are connected internally at a junction. The electrodes comprise gallium metal injected within the 3-D printed device. Voltage across the electrodes generates water-in-oil droplets, determines a rate for a series of droplets, or triggers a phase shift in the droplets. An external trigger generates the droplets based on the frequency of an XFEL utilized in droplet detection, thereby synchronizing a series of droplets with x-ray pulses for efficient crystal detection. The generated droplets can be coupled to an SFX with XFEL experiment compatible with common liquid injector such as a GDVN.
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公开(公告)号:US20190178822A1
公开(公告)日:2019-06-13
申请号:US16328262
申请日:2018-05-22
Inventor: Alexandra ROS , Gerrit BREHM , Garrett NELSON , John SPENCE , Uwe WEIERSTALL , Austin ECHELMEIER
IPC: G01N23/2005 , B01F13/00 , B33Y80/00 , G01N23/20025 , B01L3/00
CPC classification number: G01N23/2005 , B01F13/0062 , B01F2215/0037 , B01L3/5027 , B01L3/502715 , B01L2300/0867 , B33Y80/00 , G01N23/20025 , G01N2223/602 , G01N2223/604
Abstract: A 3D printed hybrid nozzle device combining a microfluidic mixer with a liquid jet injector that addresses the bottleneck of investigating substrate-initiated biological reaction paths employing serial crystallography with XFELs. The hybrid nozzle provides for injecting aqueous protein crystal jets after fast mixing (
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