公开(公告)号：US20190265212A1

公开(公告)日：2019-08-29

申请号：US16412704

申请日：2019-05-15

Applicant: ACADEMIA SINICA

Inventor： Chung-Hsuan CHEN ,  Chen-Yu HSIEH ,  Jung-Lee LIN

IPC: G01N30/74

Abstract: Apparatus and methods for high performance liquid chromatography. The apparatus includes a preparation loop comprising two linear stepping pumps, a sample loop comprising a sample injector, a chromatography column, and a detector device. The detector device can include a flash lamp, a flow cell, and a light sensor comprising an entrance slit, a grating; and a charge-coupled device array.

公开(公告)号：US20160163529A1

公开(公告)日：2016-06-09

申请号：US15046387

申请日：2016-02-17

Applicant: Academia Sinica

Inventor： Chung-Hsuan CHEN ,  I-Chung LU ,  Ming-Lee CHU

IPC: H01J49/36 ,  H01J49/04

Abstract: An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by scanning an RF frequency applied to the linear ion trap for mass selective ejection of the ions by using two power amplifiers to apply opposite phases of the RF to x and y electrodes.

公开(公告)号：US20230348566A1

公开(公告)日：2023-11-02

申请号：US18025637

申请日：2021-09-10

Applicant: Academia Sinica

Inventor： Chi-Ming LIANG ,  Jeng-Jer SHIEH ,  Chung-Hsuan CHEN ,  Shu-Mei LIANG

IPC: C07K14/765 ,  A61P35/00

CPC classification number: C07K14/765 ,  A61P35/00

Abstract: Re-folded human serum albumin (rfHSA) and use thereof for anti-tumor are disclosed. The rfHSA comprises the primary amino acid sequence of naive human serum albumin, in which the rfHSA in a solution is oval shape, not fibrillar, and the naive HSA is globular. The rfHSA is used for treating cancer or a tumor in a subject in need thereof The rfHSA may also be used as a reagent for detecting the presence of a cancer cell associated with integrin β1 or serine/threonine protein kinase Akt and extracellular signal-regulated kinase 1/2 (ERK1/2) in a tumor sample or as a reagent for inhibiting phosphorylation of Akt and ERK 1/2 in a cancer cell sample. A cell lysate of a cancer cell treated with rfHSA, a vaccine composition comprising the cancer cell lysate, and use thereof are also disclosed. Also disclosed is a method tor preparing rfHSA.

公开(公告)号：US20170183660A1

公开(公告)日：2017-06-29

申请号：US15312667

申请日：2015-05-29

Applicant: Academia Sinica

Inventor： Chung-Hsuan CHEN ,  Bai-Ling LIN ,  Chin-Yu WANG

IPC: C12N15/115

CPC classification number: C12N15/115 ,  A61K47/55 ,  C12N2310/16 ,  C12N2310/315 ,  C12N2310/3517 ,  C12N2320/13 ,  C12N2320/31 ,  C12N2320/32

Abstract: Disclosed herein is an aptamer targeting a MHC-presented peptide and its uses thereof. The MHC-presented peptide is expressed in various cancer cells; therefor, the aptamer of the disclosure is useful as a bio-tool to label and/or treat peptide-presenting cancer cells. Also disclosed herein is a pharmaceutical composition containing the aptamer.

公开(公告)号：US20210113179A1

公开(公告)日：2021-04-22

申请号：US17073408

申请日：2020-10-19

Applicant: Academia Sinica ,  National Chiao Tung University

Inventor： Chung-Hsuan CHEN ,  Shok-Li NG ,  Yung-Chieh CHAN ,  Peter LAI ,  Michael HSIAO ,  Olga K KOSHELEVA ,  Nelson G. CHEN

IPC: A61B8/08 ,  A61K49/22

Abstract: Disclosed herein is a method of enhancing the accuracy and/or sensitivity of ultrasound imaging in detecting a tumor in a subject. The method comprises administering to the subject an effective amount of a nanoparticle prior to the application of ultrasound to the subject. According to certain embodiments of the present disclosure, the nanoparticle is a magnetic nanoparticle, for example, a gold, silver, or iron oxide nanoparticle. Also disclosed herein are methods of treating a tumor in a subject by detecting the tumor via ultrasound with the aid of a nanoparticle, and then administering to the subject an anti-cancer treatment based on the location of the tumor revealed by the ultrasound image.

公开(公告)号：US20130206975A1

公开(公告)日：2013-08-15

申请号：US13691785

申请日：2012-12-01

Applicant: Academia Sinica

Inventor： Chung-Hsuan CHEN ,  Jung-Lee LIN ,  Nien-Yeen HSU ,  Yi-Sheng WANG

IPC: H05H5/04 ,  H01J49/40

CPC classification number: H05H5/047 ,  H01J49/06 ,  H01J49/40 ,  H01J49/403

Abstract: A novel system and methods for accelerating analytes including, without limitation, molecular ions, biomolecules, polymers, nano- and microparticles, is provided. The invention can be useful for increasing detection sensitivity in applications such as mass spectrometry, performing collision-induced dissociation molecular structure analysis, and probing surfaces and samples using accelerated analyte.

Abstract translation: 提供了一种用于加速分析物的新系统和方法，包括但不限于分子离子，生物分子，聚合物，纳米和微粒。 本发明可用于在诸如质谱法，进行碰撞诱导的解离分子结构分析和使用加速分析物的探测表面和样品等应用中提高检测灵敏度。

公开(公告)号：US20220000817A1

公开(公告)日：2022-01-06

申请号：US17289736

申请日：2019-11-01

Applicant: Academia Sinica

Inventor： Wei-Chao CHANG ,  Chung-Hsuan CHEN

IPC: A61K31/192 ,  C12N15/113 ,  A61K31/7105 ,  C12N15/115 ,  C07K16/18 ,  A61P35/04

Abstract: Provided herein is a method for treating a subject afflicted with a metastatic cancer. The method includes, administering to the subject an effective amount of an agent capable of suppressing the expression of a nucleic of EF-hand domain-containing protein D2 (EFHD2) or a polypeptide encoded by the nucleic acid in the metastatic cancer of the subject. The agent may be a 2-aryl propionic acid (2-APA) compound, or a short hairpin ribonucleic acid (shRNA) that directs cleavage of EFHD2 gene RNA via RNA interference. Also provided herein is a method for detecting and/or diagnosing whether a subject having a metastatic cancer via a biological sample of the subject. The method includes steps of, measuring the level of EFHD2 nucleic acid or polypeptide in the biological sample; and comparing the amount of the EFHD2 nucleic acid or polypeptide present in the biological sample with that of a healthy subject; in which an elevated amount of EFHD2 nucleic acid or polypeptide in the biological sample relative to that of a healthy subject indicates that the cancer of the subject is likely to metastasize.

公开(公告)号：US20200161110A1

公开(公告)日：2020-05-21

申请号：US16686203

申请日：2019-11-17

Applicant: Academia Sinica

Inventor： Abdil OZDEMIR ,  Jung-Lee LIN ,  Chung-Hsuan CHEN

IPC: H01J49/00 ,  H01J49/06 ,  H01J49/16

Abstract: Disclosed herein is a device for characterizing a biological sample or an airborne sample. According to embodiments of the present disclosure, the device comprises an electrospray source, a mass analyzer, a charge detector, and optionally, an ion guide. The present device is useful in analyzing the particle population in the biological or airborne sample based on the mass to charge (m/z) ratio and the charge (z) of each particle. Also disclosed herein are the methods of making a diagnosis of cancer by use of the present device, and methods of determining the mass distribution of particles in an airborne sample.

公开(公告)号：US20150160232A1

公开(公告)日：2015-06-11

申请号：US14298911

申请日：2014-06-07

Applicant: Academia Sinica

Inventor： Chung-Hsuan CHEN ,  Chein-Hung CHEN ,  Hsin Yu HSIEH ,  Pang-Hung HSU ,  Jung-Lee LIN

IPC: G01N33/68 ,  H01J49/00 ,  H01J49/42

CPC classification number: H01J49/0031 ,  G01N33/6818 ,  G01N33/6851 ,  H01J49/0054

Abstract: Apparatus and methods for automatic amino acid sequencing of a glycopeptide by mass spectrometry. The glycopeptide is fragmented by higher energy collision dissociation fragmentation, and sequentially fragmented by collision induced dissociation fragmentation. The glycopeptide Y1 ion is isolated, and the mass spectra of fragmented glycopeptide Y1 ions provide mass spectral peaks corresponding to the amino acid sequence of the glycopeptide.

Abstract translation: 通过质谱法对糖肽进行自动氨基酸测序的装置和方法。 糖肽通过较高的能量碰撞解离断裂而碎裂，并通过碰撞诱导的解离片段依次碎裂。 分离糖肽Y1离子，碎片糖肽Y1离子的质谱提供对应于糖肽的氨基酸序列的质谱峰。