公开(公告)号：US10526590B2

公开(公告)日：2020-01-07

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

公开(公告)号：US20170058298A1

公开(公告)日：2017-03-02

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3519

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract translation: 本发明涉及包含具有一个或多个修饰的衔接子部分的引导RNA，以及它们在CRISPR：Cas系统的同源重组中的用途。 修饰的衔接片段耐RNaseH降解。 本发明还涉及双指导RNA策略，其中第一引导RNA指导Cas酶在第一靶序列处进行双链断裂，并且第二引导RNA包含连接于供体多核苷酸的衔接子片段，以及 结合与第一靶序列偏移的第二靶序列。

公开(公告)号：US10538750B2

公开(公告)日：2020-01-21

申请号：US15442437

申请日：2017-02-24

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Andrew Kennedy

IPC: C12N9/22

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.

公开(公告)号：US20170247671A1

公开(公告)日：2017-08-31

申请号：US15442437

申请日：2017-02-24

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Andrew Kennedy

IPC: C12N9/22

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.

公开(公告)号：US20150367345A1

公开(公告)日：2015-12-24

申请号：US14310838

申请日：2014-06-20

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Alicia Scheffer-Wong

IPC: B01L3/00 ,  B01D21/26 ,  G01N15/10

CPC classification number: B01L3/502753 ,  B01D21/262 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/0409 ,  B01L2400/043 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/14 ,  G01N2001/4083 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A microfluidic disk for concentrating particles includes a plurality of distribution channels and separation channels. A sample fluid is flowed through the distribution channels while the disk is spun. Particles of the sample fluid flow into the separation channels where they accumulate. The particles in the separation channels may be subjected to an analysis.

Abstract translation: 用于浓缩颗粒的微流体盘包括多个分配通道和分离通道。 当盘旋转时，样品流体流过分配通道。 样品流体的颗粒流入它们积聚的分离通道。 可以对分离通道中的颗粒进行分析。

公开(公告)号：US20240409864A1

公开(公告)日：2024-12-12

申请号：US18701642

申请日：2022-11-04

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Smruti Madan Phadnis ,  Andrew C. Neilson ,  Hien Vuong Cheung

IPC: C12M1/32 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

Abstract: An apparatus for containing a three-dimensional cellular material surrounded by a medium that facilitates and maintains the centering of the three-dimensional cellular material throughout an assay is provided. The apparatus includes a well having an open proximal end and a closed distal end. Further, the well defines a compartment having an interior surface and a sample nesting site for containing the three-dimensional cellular material surrounded by the medium. A central indentation is located at the closed distal end of the well, a first concentric lip is located above the central indentation in a y-direction towards the open proximal end of the well, and a second concentric lip is located above the first concentric lip in the y-direction towards the open proximal end of the well. Additionally, the first concentric lip and the second concentric lip define a groove therebetween. A method of forming a three-dimensional cellular material is also provided.

公开(公告)号：US20190247847A1

公开(公告)日：2019-08-15

申请号：US15896255

申请日：2018-02-14

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Genevieve Van De Bittner ,  Smruti Madan Phadnis

IPC: B01L3/00 ,  C12M1/32 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  G01N21/64

CPC classification number: B01L3/5025 ,  B01L3/50853 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0829 ,  C12M23/12 ,  C12M25/04 ,  C12M27/18 ,  C12M29/10 ,  C12M41/32 ,  G01N21/6454

Abstract: The invention relates generally to methods and apparatus that measure one or more properties of an individual cell type in a non-contact co-culture. More specifically, the invention relates to a novel multiwell plate that allows separate but simultaneous metabolic measurements of cell populations in non-contact co culture.