公开(公告)号：US20250051822A1

公开(公告)日：2025-02-13

申请号：US18720123

申请日：2022-12-19

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Christopher Sauer ,  Mathis Appelbaum ,  Stefan Jenewein ,  Sebastian Schoof ,  Christian Riedele

IPC: C12P21/02 ,  C12N9/38 ,  C12N15/75

Abstract: The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.

公开(公告)号：US10513693B2

公开(公告)日：2019-12-24

申请号：US15127181

申请日：2015-03-18

Applicant: BASF SE

Inventor： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC: C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C07K14/285

Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).

公开(公告)号：US20180030418A1

公开(公告)日：2018-02-01

申请号：US15127181

申请日：2015-03-18

Applicant: BASF SE

Inventor： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC: C12N9/04 ,  C12N9/10

CPC classification number: C12N9/0006 ,  C07K14/285 ,  C12N9/1029 ,  C12P7/46 ,  C12Y101/01027 ,  C12Y203/01054

Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).