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公开(公告)号：US20230295603A1
公开(公告)日：2023-09-21
申请号：US18017430
申请日：2021-07-23
申请人： BASF SE
CPC分类号： C12N9/90 , C12N15/75 , C12Y501/01001 , C12N2800/101
摘要： The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20230272358A1
公开(公告)日：2023-08-31
申请号：US18017426
申请日：2021-07-23
申请人： BASF SE
CPC分类号： C12N9/48 , C12N15/75 , C12N9/90 , C12N1/20 , C12R2001/10
摘要： The present invention relates to a Bacillus host cell belonging to the species Bacillus lichemformis or Bacillus pumilus in which the chromosomal alr gene has been inactivated. Said bacterial host cell comprises a plasmid comprising at least one autonomous replication sequence, a first polynucleotide encoding at least one polypeptide of interest, wherein said first polynucleotide is operably linked to a promoter, and a second polynucleotide encoding an alanine racemase which is not native to the host cell, wherein said second polynucleotide is operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20240060110A1
公开(公告)日：2024-02-22
申请号：US18030700
申请日：2021-10-06
申请人： BASF SE
CPC分类号： C12P21/02 , C12Y301/01001 , C12N9/20 , C12Y301/02002 , C12N9/16
摘要： The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.
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4.发明公开公开(公告)号：US20240318188A1
公开(公告)日：2024-09-26
申请号：US18572827
申请日：2022-06-24
申请人： BASF SE
摘要： The present invention relates to a Bacillus licheniformis host cell for production of biological compounds with increased purity. Specifically, the invention relates to a Bacillus licheniformis host with one or more genetic modifications selected from a) a genetic modification causing compared to an unmodified control cell an increased expression of at least one of the genes selected from the group consisting of degQ, degll, degS, degR, and phrG, b) a genetic modification causing compared to an unmodified control cell an increased autophosphorylation activity of the DegS protein, and c) a genetic modification causing compared to an unmodified control cell a reduced phosphatase activity of the DegS protein. The present invention further relates to a method for production of at least one compound of interest with increased purity based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20240317820A1
公开(公告)日：2024-09-26
申请号：US18572974
申请日：2022-06-24
申请人： BASF SE
发明人： Max Fabian Felle , Christopher Sauer , Mathis Appelbaum , Stefan Jenewein , Tobias Klein , Thomas Schweder
摘要： The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications that lead to decreased production of components of biofilm and increased levels of phosphorylated De-gll. Said Bacillus host comprises at least one gene expression cassette with a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter for the production of compounds. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20240294894A1
公开(公告)日：2024-09-05
申请号：US18572942
申请日：2022-06-24
申请人： BASF SE
CPC分类号： C12N9/54 , C12N15/75 , C12Y304/21062 , C12N2800/101
摘要： The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications in the remA and/or remB gene. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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