公开(公告)号：US20140356916A1

公开(公告)日：2014-12-04

申请号：US14365660

申请日：2012-12-14

Applicant: BASF SE

Inventor： Christoph Wittmann ,  Stefanie Kind ,  Judith Becker

IPC: C12P13/08 ,  C12P13/00

CPC classification number: C12P13/08 ,  C12N9/93 ,  C12P13/001 ,  C12Y602/01004 ,  C12Y602/01005 ,  Y02P20/52

Abstract: Recombinant microorganisms with deregulated succinyl-CoA synthetase activity, as well as the uses for producing lysine, β-lysine, cadaverine or N-acetylcadaverine thereby are provided. Recombinant polypeptides comprising an amino acid sequence being at least 80% identical to SEQ ID NO: 51 or 53 are also provided. The method of producing fine chemicals using said recombinant microorganisms, in particular the method of producing lysine, or derivatives thereof, such as β-lysine, cadaverine or N-acetylcadaverine is further provided.

Abstract translation: 提供了具有失控的琥珀酰-CoA合成酶活性的重组微生物，以及用于产生赖氨酸，赖氨酸，尸胺或N-乙酰胆碱酯酶的用途。 还提供了包含与SEQ ID NO：51或53至少80％相同的氨基酸序列的重组多肽。 进一步提供使用所述重组微生物生产精细化学品的方法，特别是生产赖氨酸的方法或其衍生物，例如β-赖氨酸，尸胺或N-乙酰基尸胺。

公开(公告)号：US10273485B2

公开(公告)日：2019-04-30

申请号：US15116600

申请日：2015-02-06

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC: C12N1/20 ,  C12N15/52 ,  C12N1/02 ,  C12N9/12 ,  C12P7/46 ,  C12N9/04 ,  C12N9/10 ,  C12N15/74

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

公开(公告)号：US09644220B2

公开(公告)日：2017-05-09

申请号：US14365660

申请日：2012-12-14

Applicant: BASF SE

Inventor： Christoph Wittmann ,  Stefanie Kind ,  Judith Becker

IPC: C12P13/08 ,  C12P13/00 ,  C12N9/00

CPC classification number: C12P13/08 ,  C12N9/93 ,  C12P13/001 ,  C12Y602/01004 ,  C12Y602/01005 ,  Y02P20/52

Abstract: Recombinant microorganisms with deregulated succinyl-CoA synthetase activity, as well as the uses for producing lysine, β-lysine, cadaverine or N-acetylcadaverine thereby are provided. Recombinant polypeptides comprising an amino acid sequence being at least 80% identical to SEQ ID NO: 51 or 53 are also provided. The method of producing fine chemicals using said recombinant microorganisms, in particular the method of producing lysine, or derivatives thereof, such as β-lysine, cadaverine or N-acetylcadaverine is further provided.

公开(公告)号：US10287558B2

公开(公告)日：2019-05-14

申请号：US15116669

申请日：2015-02-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker ,  Anna Lange ,  Benjamin J. Lyons ,  Thomas J. Lyons ,  Eudes De Crecy ,  Ewa Hughes

IPC: C12N9/12 ,  C12P7/46 ,  C12N15/52

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

公开(公告)号：US20160362696A1

公开(公告)日：2016-12-15

申请号：US15116600

申请日：2015-02-06

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C12N9/12

CPC classification number: C12N15/52 ,  C12N1/02 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01 ,  C12Y203/01054 ,  C12Y207/08031

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

Abstract translation: 本发明涉及与其野生型相比具有由wcaJ基因编码的酶的活性降低的修饰微生物。 本发明还涉及有机化合物的制造方法和改性微生物的用途。

公开(公告)号：US20160348082A1

公开(公告)日：2016-12-01

申请号：US15116669

申请日：2015-02-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oakar Zelder ,  Gregory von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker ,  Anna Lange ,  Benjamin J. Lyons ,  Thomas J. Lyons ,  Eudes De Crecy ,  Ewa Hughes

IPC: C12N9/12 ,  C12P7/46

CPC classification number: C12N9/1205 ,  C12N15/52 ,  C12P7/46 ,  C12Y207/0104

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

Abstract translation: 本发明涉及与其野生型相比具有由pykA基因编码的酶的活性降低的修饰微生物。 本发明还涉及一种生产有机化合物的方法和使用改性微生物。