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公开(公告)号：US12241069B2
公开(公告)日：2025-03-04
申请号：US18317915
申请日：2023-05-15
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Arash Farhadi , Gabrielle H Ho , Daniel P Sawyer , Mikhail Shapiro , Robert C. Hurt , Mengtong Duan
IPC: C12N15/67 , C07K14/195
Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
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公开(公告)号：US11649462B2
公开(公告)日：2023-05-16
申请号：US16736683
申请日：2020-01-07
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Arash Farhadi , Gabrielle H Ho , Daniel P Sawyer , Mikhail Shapiro , Robert C. Hurt , Mengtong Duan
IPC: C12N15/67 , C07K14/195
CPC classification number: C12N15/67 , C07K14/195 , C12N2510/02
Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
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公开(公告)号：US20230210926A1
公开(公告)日：2023-07-06
申请号：US18066814
申请日：2022-12-15
Applicant: California Institute of Technology
Inventor： Marjorie T. Buss , Robert C. Hurt , Katie K. Wong , Mikhail G. Shapiro , Mengtong Duan , Arash Farhadi , Mei Yi You
IPC: A61K35/748 , C07K14/195 , A61K49/22 , A61P35/00
CPC classification number: A61K35/748 , A61K49/22 , A61P35/00 , C07K14/195 , A61K2035/115
Abstract: Disclosed herein include methods, compositions, and kits suitable for use in dynamic non-destructive imaging. The non-destructive imaging can be nonlinear ultrasound imaging. There are provided, in some embodiments, nucleic acid compositions encoding gas vesicles (GVs) capable of producing nonlinear ultrasound contrast upon expression in a prokaryotic cell (e.g., a probiotic bacterial cell) or a eukaryotic cell (e.g., a therapeutic mammalian cell).
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4.发明授权公开(公告)号：US11504438B2
公开(公告)日：2022-11-22
申请号：US16656417
申请日：2019-10-17
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Anupama Lakshmanan , Arash Farhadi , Suchita P. Nety , Raymond W. Bourdeau , Mikhail Shapiro
IPC: A61K49/22 , C07K14/195 , A61K49/00 , A61B8/08
Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.
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公开(公告)号：US11118210B2
公开(公告)日：2021-09-14
申请号：US15663635
申请日：2017-07-28
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Raymond W. Bourdeau , Anupama Lakshmanan , Arash Farhadi , Mikhail G. Shapiro , Audrey Lee-Gosselin
Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.
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Patent Agency Ranking
6.发明授权公开(公告)号：US10955496B2
公开(公告)日：2021-03-23
申请号：US15663600
申请日：2017-07-28
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： George J. Lu , Mikhail G. Shapiro , Arash Farhadi , Jerzy O. Szablowski
Abstract: Gas vesicle protein structures and related compositions, methods, and systems for singleplexed and/or multiplexed magnetic resonance imaging of a target site alone or in combination with ultrasound are described, in which the gas vesicle protein structures provide contrast for the imaging.
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公开(公告)号：US12209268B2
公开(公告)日：2025-01-28
申请号：US17334953
申请日：2021-05-31
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Raymond W. Bourdeau , Anupama Lakshmanan , Arash Farhadi , Mikhail G. Shapiro , Audrey Lee-Gosselin
Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.
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公开(公告)号：US11786218B2
公开(公告)日：2023-10-17
申请号：US16736581
申请日：2020-01-07
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Daniel P. Sawyer , Arash Farhadi , Avinoam D. Bar-Zion , Mikhail Shapiro
IPC: A61B8/08
CPC classification number: A61B8/5207 , A61B8/481 , A61B8/5246
Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.
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公开(公告)号：US11761008B2
公开(公告)日：2023-09-19
申请号：US16736683
申请日：2020-01-07
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Arash Farhadi , Gabrielle H Ho , Daniel P Sawyer , Mikhail Shapiro , Robert C. Hurt , Mengtong Duan
IPC: C12N15/67 , C07K14/195
CPC classification number: C12N15/67 , C07K14/195 , C12N2510/02
Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
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公开(公告)号：US11653899B2
公开(公告)日：2023-05-23
申请号：US16736581
申请日：2020-01-07
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Daniel P. Sawyer , Arash Farhadi , Avinoam D. Bar-Zion , Mikhail Shapiro
IPC: A61B8/08
CPC classification number: A61B8/5207 , A61B8/481 , A61B8/5246
Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.
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