公开(公告)号：US20230260775A1

公开(公告)日：2023-08-17

申请号：US18016173

申请日：2021-07-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi BABA ,  Grant GODBEHERE

IPC: H01J49/42 ,  H01J49/40

CPC classification number: H01J49/422 ,  H01J49/427 ,  H01J49/421 ,  H01J49/40

Abstract: In one aspect, a method of performing mass spectrometry is disclosed, which comprises ionizing a sample to generate a plurality of precursor ions, passing the precursor ions through a mass filter to select at least one subset of the ions, introducing the selected ions into a branched radiofrequency (RF) ion trap and subjecting at least a portion of said selected precursor ions to fragmentation within the ion trap so as to generate a first plurality of fragment ions. The method can further include isolating at least a portion of the first plurality of fragment ions in at least one branch of the branched RF ion trap, removing unwanted fragment ions, releasing the remaining ions from said at least one branch and subjecting at least a portion thereof to fragmentation so as to generate a second plurality of fragment ions. Any combination of collision induced dissociation (CID) and electron activation dissociation (EAD) can be employed for fragmenting the ions.

公开(公告)号：US20210351026A1

公开(公告)日：2021-11-11

申请号：US17284336

申请日：2019-10-08

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi BABA ,  Pavel RYUMIN

IPC: H01J49/00 ,  H01J49/02

Abstract: In one aspect, an electron-ion reaction module, e.g., an electron capture dissociation module, for use in a mass spectrometer is disclosed, which comprises a chamber, an electron source for generating electrons and introducing the electrons into the chamber, a gate electrode positioned relative to the electron source and the chamber, and a DC voltage source operatively coupled to the gate electrode for applying control voltages to the gate electrode. The electron-ion interaction module can further include a controller operably coupled to the DC voltage source and configured for adjusting the DC voltage applied to the gate electrode to adjust flow of electrons into the chamber.

公开(公告)号：US20200279727A1

公开(公告)日：2020-09-03

申请号：US16063104

申请日：2016-12-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi BABA ,  Paul BAKER ,  John Lawrence CAMPBELL ,  Yves LeBLANC

IPC: H01J49/00 ,  G01N33/92

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds and/or the orientation of those double bonds within the sample molecules. The fragments may include radical fragments and non-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules as confirmation of the presence of those radical fragments.

公开(公告)号：US20170358437A1

公开(公告)日：2017-12-14

申请号：US15531367

申请日：2015-11-24

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi BABA ,  Alex BERYLAND

IPC: H01J49/06 ,  H01J49/38

CPC classification number: H01J49/063 ,  H01J49/38

Abstract: Methods and systems for analyzing ions in a magnetic ion trap are provided herein. In accordance with various aspects of the present teachings, the methods and systems described herein enable Fourier transform ion cyclotron resonance mass spectrometry across relatively narrow gap magnetic fields substantially perpendicular to the axis along which the ions are injected into the ion trap. As a result, smaller, less expensive magnets can be used to produce the high-intensity, uniform magnetic fields utilized in high performance FT-ICR/MS applications. Accordingly, the present teachings enable permanent magnets (as well as electromagnets) to generate these magnetic fields, potentially reducing the cost, size, and/or complexity of the systems described herein relative to conventional FT-ICR systems.

公开(公告)号：US20180005810A1

公开(公告)日：2018-01-04

申请号：US15540750

申请日：2015-12-21

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi BABA

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0072 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/063

Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.

公开(公告)号：US20240222103A1

公开(公告)日：2024-07-04

申请号：US18569773

申请日：2022-06-16

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi BABA ,  Keqin CHEN

IPC: H01J49/00 ,  H01J49/02 ,  H01J49/08 ,  H01J49/36

CPC classification number: H01J49/0054 ,  H01J49/022 ,  H01J49/08 ,  H01J49/36

Abstract: In one aspect, an electron capture dissociation (ECD) device for use in a mass spectrometer is disclosed, which is configured to trap precursor ions and cause the trapped precursor ions (or a portion thereof) to exit the ion trap, via radial excitation thereof by a resonant AC voltage, such that the released precursor ions can enter an ion-electron interaction region in which at least a portion of the precursor ions undergo fragmentation via interaction with an electron beam. The fragment ions are trapped and prevented from undergoing multiple dissociations. Once the fragmentation of the precursor ions is completed and/or after a predefined period, the fragment ions are released from the ECD to be received by downstream components of the mass spectrometer in which the ECD device is incorporated.

公开(公告)号：US20230360900A1

公开(公告)日：2023-11-09

申请号：US18025511

申请日：2021-09-08

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi BABA

IPC: H01J49/00

CPC classification number: H01J49/0054

Abstract: An ion sequestering apparatus and methods or systems using one or more auxiliary electrodes in an ion reaction instrument having RF electrodes adapted to guide positively-charged precursor ions along a first axis, and an electron source for introduction of an electron beam along a second axis transverse to the first axis such that electron activated dissociation of the precursor ions into reaction products can occur, the auxiliary electrode configured to apply a supplemental AC signal to permit selective extraction of reaction products while sequestering precursor ions along the second central axis. For example, the supplemental AC signal can comprises an notched white noise signal with a notch that suppresses frequencies at which the precursor ions (and/or charge reduced species that have the same molecular mass but have a different charge state) would otherwise be excited.

公开(公告)号：US20160126076A1

公开(公告)日：2016-05-05

申请号：US14894359

申请日：2014-05-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi BABA

IPC: H01J49/00

CPC classification number: H01J49/0054 ,  H01J49/0045 ,  H01J49/0072 ,  H01J49/063

Abstract: A method and apparatus for conducting ion to charged species reactions, more particularly reactions wherein the charged species is an electron, such as ECD. The apparatus comprises first and second pathways which are orthogonal to one another. The first pathway through which ions are introduced comprises multiple multipoles with a gap situated there between. The second pathway introduces the charged species through the gap orthogonally to the first pathway. In this way, a cross-type reaction device allows ion-charged species interactions to occur.

Abstract translation: 用于将离子引入带电物种反应的方法和装置，更具体地说，其中带电物质是电子的反应，例如ECD。 该装置包括彼此正交的第一和第二路径。 离子引入的第一条路径包括多个多极，其间有间隙。 第二路径通过与第一路径正交的间隙引入带电物质。 以这种方式，交叉型反应装置允许发生离子充电的物质相互作用。

公开(公告)号：US20230343570A1

公开(公告)日：2023-10-26

申请号：US18016162

申请日：2021-07-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi BABA ,  Kaoru KARASAWA

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0045 ,  H01J49/062

Abstract: In one aspect, a method of performing mass spectrometry is disclosed, which comprises ionizing a plurality of oligonucleotides to generate a plurality of negatively charged oligonucleotide ions, and interacting a plurality of charged reagent ions with the negatively charged oligonucleotide ions to reduce the negative charge state of the negatively charged oligonucleotide ions.