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公开(公告)号：US11136600B2
公开(公告)日：2021-10-05
申请号：US15766184
申请日：2016-10-03
Applicant: DSM IP Assets B.V.
Inventor： Ioannis Papapetridis , Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Paul Klaassen
IPC: C12P7/02 , C12P7/10 , C12N1/18 , C12N9/00 , C12N9/04 , C12N9/02 , C12N9/12 , C12N9/18 , C12N15/52
Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
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公开(公告)号：US11078504B2
公开(公告)日：2021-08-03
申请号：US16063810
申请日：2016-12-22
Applicant: DSM IP ASSETS B.V.
Inventor： Alrik Pieter Los , Zheng Zhao , René Verwaal , Jacobus Thomas Pronk , Rolf Poldermans
Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
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公开(公告)号：US20170321242A1
公开(公告)日：2017-11-09
申请号：US15657042
申请日：2017-07-21
Applicant: DSM IP ASSETS B.V.
Inventor： Hubertus Johannes Marie OP DEN CAMP , Harry Ramanoedj Harhangi , Christiaan Van Der Drift , Jacobus Thomas Pronk
IPC: C12P35/00 , C12N9/90 , C12P17/10 , C12P13/00 , C12P7/56 , C12N9/92 , C12N9/12 , C12P7/20 , C12P7/18 , C12P7/06 , C12P7/00 , C12P5/02 , C12P7/46 , C12P7/54
CPC classification number: C12P35/00 , C12N9/1205 , C12N9/90 , C12N9/92 , C12P5/026 , C12P7/00 , C12P7/06 , C12P7/18 , C12P7/20 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/001 , C12P17/10 , C12Y207/01017 , C12Y503/01005 , Y02E50/16 , Y02E50/17
Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.
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公开(公告)号：US09528117B2
公开(公告)日：2016-12-27
申请号：US14189989
申请日：2014-02-25
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
CPC classification number: C12N15/815 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12P7/06 , C12P7/10 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y602/01001 , Y02E50/16 , Y02E50/17 , Y02P20/52
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
Abstract translation: 本发明涉及酵母细胞，特别是重组酵母细胞，NADH依赖性甘油合成所需的缺乏酶活性的细胞或相对于NADH依赖性甘油合成具有降低的酶活性的细胞与其相应的 野生型酵母细胞，该细胞包含编码NAD +依赖性乙酰化乙醛脱氢酶（EC 1.2.1.10）活性的一种或多种异源核酸序列。 本发明还涉及根据本发明的细胞在制备乙醇中的用途。
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公开(公告)号：US09023629B2
公开(公告)日：2015-05-05
申请号：US13688418
申请日：2012-11-29
Applicant: DSM IP Assets B.V.
Inventor： Hubertus Johannes Marie Op Den Camp , Harry Ramanoedj Harhangi , Christiaan Van Der Drift , Jacobus Thomas Pronk
IPC: C12P7/06 , C12N9/00 , C12N9/90 , C12N1/20 , C12N15/00 , C07H21/04 , C12P35/00 , C12N9/12 , C12N9/92 , C12P7/00 , C12P5/02 , C12P7/18 , C12P7/20 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/00 , C12P17/10
CPC classification number: C12P35/00 , C12N9/1205 , C12N9/90 , C12N9/92 , C12P5/026 , C12P7/00 , C12P7/06 , C12P7/18 , C12P7/20 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/001 , C12P17/10 , C12Y207/01017 , C12Y503/01005 , Y02E50/16 , Y02E50/17
Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.
Abstract translation: 本发明涉及用编码从厌氧真菌获得的真核木糖异构酶的核酸序列转化的宿主细胞。 当表达时，编码木糖异构酶的序列赋予宿主细胞将木糖转化成木酮糖的能力，其可以被宿主细胞进一步代谢。 因此，宿主细胞能够在木糖上生长作为碳源。 宿主细胞优选是真核微生物，例如酵母或丝状真菌。 本发明还涉及用于生产发酵产物如乙醇的方法，其中本发明的宿主细胞使用木糖进行生长和产生发酵产物。 本发明还涉及从厌氧真菌获得的编码真核木糖异构酶和木酮糖激酶的核酸序列。
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Patent Agency Ranking
公开(公告)号：US11326175B2
公开(公告)日：2022-05-10
申请号：US17176073
申请日：2021-02-15
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US11326172B2
公开(公告)日：2022-05-10
申请号：US16763394
申请日：2018-11-12
Applicant: DSM IP ASSETS B.V.
Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.
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公开(公告)号：US11174489B2
公开(公告)日：2021-11-16
申请号：US17176040
申请日：2021-02-15
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US10738317B2
公开(公告)日：2020-08-11
申请号：US15970641
申请日：2018-05-03
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US10689670B2
公开(公告)日：2020-06-23
申请号：US16309422
申请日：2017-06-13
Applicant: DSM IP ASSETS B.V.
Inventor： Ioannis Papapetridis , Antonius Jeroen Adriaan Van Maris , Jacobus Thomas Pronk , Paulus Petrus De Waal , Niels Lauret , Maarten Dirk Verhoeven , Jozef Petrus Johannes Schmitz
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
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