公开(公告)号：US11078504B2

公开(公告)日：2021-08-03

申请号：US16063810

申请日：2016-12-22

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Zheng Zhao ,  René Verwaal ,  Jacobus Thomas Pronk ,  Rolf Poldermans

IPC: C12P7/46 ,  C12N9/04 ,  C12N9/02 ,  C12N15/81

Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US09944905B2

公开(公告)日：2018-04-17

申请号：US15103237

申请日：2014-12-12

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel De Jong ,  Zheng Zhao ,  Ben Den Dulk ,  Remko Tsjibbe Winter

IPC: C12P7/62 ,  C12P7/46 ,  C12N9/02 ,  C12P19/34 ,  C12P21/06 ,  C12N15/00 ,  C12N1/00 ,  C07H21/04 ,  C07K1/00

CPC classification number: C12N9/001 ,  C12P7/46 ,  C12Y103/01006

Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.

公开(公告)号：US20150291986A1

公开(公告)日：2015-10-15

申请号：US14441902

申请日：2013-11-25

Applicant: DSM IP ASSETS B.V.

Inventor： Zheng Zhao ,  Bernard Meijrink ,  Robertus Antonius Mijndert Van Der Hoeven ,  Liang Wu ,  Johannes Andries Roubos

IPC: C12P7/46 ,  C12P7/62 ,  C12N9/88 ,  C12N15/81

CPC classification number: C12P7/46 ,  C12N9/88 ,  C12N15/81 ,  C12P7/62 ,  C12Y401/01006

Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.

Abstract translation: 本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。 本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞：编码具有顺式乌头酸脱羧酶活性的多肽的核酸; 以及编码催化向乙酰辅酶A反应的多肽的核酸。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。