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公开(公告)号：US20160102331A1
公开(公告)日：2016-04-14
申请号：US14893284
申请日：2014-06-02
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius BOER , Nicolette Jasmijn BROERS , Adam G. LAWRENCE
CPC classification number: C12P19/56 , A23K20/105 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0042 , C12N9/0073 , C12N9/0083 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P15/00 , C12Y114/13 , C12Y114/13078 , C12Y402/03019 , C12Y505/01013
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.
Abstract translation: 本发明涉及二萜或糖基二萜的制备方法，该方法包括：a。 在合适的发酵培养基中发酵重组微生物，其中所述微生物包含一个或多个核苷酸序列，所述核苷酸序列编码：具有入 - copalyl焦磷酸合酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力，从而在发酵培养基中细胞外产生二萜或糖基化二萜; 和b。 从发酵培养基中回收二萜或糖基化二萜。
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公开(公告)号：US20190226000A1
公开(公告)日：2019-07-25
申请号：US16299960
申请日：2019-03-12
Applicant: DSM IP ASSETS B.V.
Inventor： Nicolette Jasmijn BROERS , Viktor Marius BOER , Adam G. LAWRENCE
IPC: C12P19/56 , C12P15/00 , C12N9/02 , C12N9/10 , C07H15/256 , C07C62/32 , A23L27/30 , A23L2/60 , C12N15/52 , C12N9/90 , C12N9/88
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.
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Patent Agency Ranking
公开(公告)号：US20190010508A1
公开(公告)日：2019-01-10
申请号：US16068395
申请日：2017-01-06
Applicant: Adam G. LAWRENCE , Peter Louis HOUSTON , Jessica Leigh MCGRATH , Joshua TRUEHEART , DSM IP Assets B.V.
Inventor： Adam G. LAWRENCE , Peter Louis HOUSTON , Jessica Leigh MCGRATH , Joshua TRUEHEART
Abstract: The present invention is directed to a process for switching the mating type of a Yarrowia fungus strain into an opposite mating type, and to the use thereof to sexually cross two individual strains resulting in new strains.
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公开(公告)号：US20180327797A1
公开(公告)日：2018-11-15
申请号：US15774721
申请日：2016-11-14
Applicant: DSM IP ASSETS B.V.
Inventor： Adam G. LAWRENCE , Celine ViAROUGE
CPC classification number: C12P19/38 , A23L33/15 , A23V2002/00 , C12N9/14 , C12N9/93 , C12Y306/01022 , C12Y603/01005
Abstract: The present disclosure relates to a novel method, expression vectors, and host cells for producing nicotinamide riboside by regulating the pathways that lead to the production of nicotinamide riboside.
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公开(公告)号：US20200024609A1
公开(公告)日：2020-01-23
申请号：US16415257
申请日：2019-05-17
Applicant: DSM IP Assets B.V.
Inventor： Adam G. LAWRENCE , Peter Louis Houston , Jessica Leigh MCGRATH , Joshua Trueheart
Abstract: The present invention is directed to a process for switching the mating type of a Yarrowia fungus strain into an opposite mating type, and to the use thereof to sexually cross two individual strains resulting in new strains.
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公开(公告)号：US20190071680A1
公开(公告)日：2019-03-07
申请号：US16084305
申请日：2017-03-16
Applicant: DSM IP Assets B.V.
Inventor： Adam G. LAWRENCE , Celine VIAROUGE
IPC: C12N15/52 , A23L33/135 , A23L33/15 , C12N9/06 , C12N9/12 , C12N9/10 , C12N9/82 , C12R1/19 , C12R1/125 , C12R1/15
Abstract: The present invention relates to a novel method, expression vectors, and host cells for producing nicotinic acid riboside by regulating the pathways that lead to the production of nicotinic acid riboside.
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公开(公告)号：US20160160257A1
公开(公告)日：2016-06-09
申请号：US14906497
申请日：2014-07-23
Applicant: DSM IP ASSETS B.V.
Inventor： Nicolette Jasmijn BROERS , Viktor Marius BOER , Adam G. LAWRENCE
CPC classification number: C12P19/56 , A23L2/60 , A23L27/36 , C07C62/32 , C07C2603/86 , C07H15/256 , C12N9/0042 , C12N9/0073 , C12N9/0083 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P15/00 , C12Y114/13078 , C12Y114/13079 , C12Y402/03019 , C12Y505/01013
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.
Abstract translation: 本发明涉及二萜或糖基二萜的制备方法，该方法包括：a。 在约29℃或更高的温度下在合适的发酵培养基中发酵耶氏酵母属的重组微生物，其中所述微生物包含一个或多个核苷酸序列，所述核苷酸序列编码：具有共 - 酰基焦磷酸合酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力; 和b。 回收二萜或糖基化二萜。
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