公开(公告)号：US08575312B2

公开(公告)日：2013-11-05

申请号：US13446254

申请日：2012-04-13

申请人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

发明人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

IPC分类号： C07K14/00

CPC分类号： C07K16/243 ,  A61K38/00 ,  C07K14/53

摘要： Disclosed is a newly identified secreted molecule, identified herein as “monocyte, granulocyte, and dendritic cell colony stimulating factor” (MGD-CSF), the polypeptide sequence, and polynucleotides encoding the polypeptide sequence. Also provided is a procedure for producing the polypeptide by recombinant techniques employing, for example, vectors and host cells. Additionally, procedures are described to modify the disclosed novel molecules of the invention to prepare fusion molecules. Also disclosed are methods for using the polypeptides and active fragments thereof for treatment of a variety of diseases, including, for example, cancer, autoimmune and inflammatory diseases, infectious diseases, and recurrent pregnancy loss.

摘要翻译： 公开了一种新鉴定的分泌型分子，本文称为“单核细胞，粒细胞和树突细胞集落刺激因子”（MGD-CSF），多肽序列和编码多肽序列的多核苷酸。 还提供了通过使用例如载体和宿主细胞的重组技术产生多肽的方法。 另外，描述了修饰所公开的本发明新颖分子以制备融合分子的方法。 还公开了将多肽及其活性片段用于治疗各种疾病（包括例如癌症，自身免疫性和炎性疾病，感染性疾病和复发性妊娠损失）的方法。

公开(公告)号：US20110003384A1

公开(公告)日：2011-01-06

申请号：US12370559

申请日：2009-02-12

申请人： Dirk BEHRENS ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

发明人： Dirk BEHRENS ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

IPC分类号： C12N5/071

CPC分类号： C07K16/243 ,  A61K38/00 ,  C07K14/53

摘要： Disclosed is a newly identified secreted molecule, identified herein as “monocyte, granulocyte, and dendritic cell colony stimulating factor” (MGD-CSF), the polypeptide sequence, and polynucleotides encoding the polypeptide sequence. Also provided is a procedure for producing the polypeptide by recombinant techniques employing, for example, vectors and host cells. Additionally, procedures are described to modify the disclosed novel molecules of the invention to prepare fusion molecules. Also disclosed are methods for using the polypeptides and active fragments thereof for treatment of a variety of diseases, including, for example, cancer, autoimmune and inflammatory diseases, infectious diseases, and recurrent pregnancy loss.

摘要翻译： 公开了一种新鉴定的分泌型分子，本文称为“单核细胞，粒细胞和树突细胞集落刺激因子”（MGD-CSF），多肽序列和编码多肽序列的多核苷酸。 还提供了通过使用例如载体和宿主细胞的重组技术产生多肽的方法。 另外，描述了修饰所公开的本发明新颖分子以制备融合分子的方法。 还公开了将多肽及其活性片段用于治疗各种疾病（包括例如癌症，自身免疫性和炎性疾病，感染性疾病和复发性妊娠损失）的方法。

公开(公告)号：US08178109B2

公开(公告)日：2012-05-15

申请号：US12370559

申请日：2009-02-12

申请人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

发明人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

IPC分类号： A61K39/00

CPC分类号： C07K16/243 ,  A61K38/00 ,  C07K14/53

摘要： Disclosed is a newly identified secreted molecule, identified herein as “monocyte, granulocyte, and dendritic cell colony stimulating factor” (MGD-CSF), the polypeptide sequence, and polynucleotides encoding the polypeptide sequence. Also provided is a procedure for producing the polypeptide by recombinant techniques employing, for example, vectors and host cells. Additionally, procedures are described to modify the disclosed novel molecules of the invention to prepare fusion molecules. Also disclosed are methods for using the polypeptides and active fragments thereof for treatment of a variety of diseases, including, for example, cancer, autoimmune and inflammatory diseases, infectious diseases, and recurrent pregnancy loss.

摘要翻译： 公开了一种新鉴定的分泌型分子，本文称为“单核细胞，粒细胞和树突细胞集落刺激因子”（MGD-CSF），多肽序列和编码多肽序列的多核苷酸。 还提供了通过使用例如载体和宿主细胞的重组技术产生多肽的方法。 另外，描述了修饰所公开的本发明新颖分子以制备融合分子的方法。 还公开了将多肽及其活性片段用于治疗各种疾病（包括例如癌症，自身免疫性和炎性疾病，感染性疾病和复发性妊娠损失）的方法。

公开(公告)号：US20120258071A1

公开(公告)日：2012-10-11

申请号：US13446254

申请日：2012-04-13

申请人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

发明人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

IPC分类号： A61K38/19 ,  C07K17/02 ,  A61P35/00 ,  A61P37/00 ,  A61P31/00 ,  C07K17/00 ,  C07K19/00

CPC分类号： C07K16/243 ,  A61K38/00 ,  C07K14/53

摘要： Disclosed is a newly identified secreted molecule, identified herein as “monocyte, granulocyte, and dendritic cell colony stimulating factor” (MGD-CSF), the polypeptide sequence, and polynucleotides encoding the polypeptide sequence. Also provided is a procedure for producing the polypeptide by recombinant techniques employing, for example, vectors and host cells. Additionally, procedures are described to modify the disclosed novel molecules of the invention to prepare fusion molecules. Also disclosed are methods for using the polypeptides and active fragments thereof for treatment of a variety of diseases, including, for example, cancer, autoimmune and inflammatory diseases, infectious diseases, and recurrent pregnancy loss.

摘要翻译： 公开了一种新鉴定的分泌型分子，本文鉴定为单核细胞，粒细胞和树突细胞集落刺激因子（MGD-CSF），多肽序列和编码多肽序列的多核苷酸。 还提供了通过使用例如载体和宿主细胞的重组技术产生多肽的方法。 另外，描述了修饰所公开的本发明新颖分子以制备融合分子的方法。 还公开了将多肽及其活性片段用于治疗各种疾病（包括例如癌症，自身免疫性和炎性疾病，感染性疾病和复发性妊娠损失）的方法。

公开(公告)号：US20110020375A1

公开(公告)日：2011-01-27

申请号：US12752936

申请日：2010-04-01

申请人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

发明人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

IPC分类号： A61K38/17 ,  C12N15/12 ,  C07K14/435 ,  C12N15/00 ,  C07K1/00 ,  C07K16/18 ,  G01N33/48 ,  G01N33/53 ,  A61K35/12 ,  A61K39/00 ,  C12N5/00 ,  A61P7/02 ,  A61P3/10 ,  A61P9/00 ,  A61P37/00 ,  A61P31/00 ,  A61P9/10 ,  A61P35/00 ,  A61P37/08 ,  A61P17/00 ,  A61P11/00

CPC分类号： C07K14/522 ,  Y10T436/143333

摘要： Disclosed herein is a newly identified SDF-1 splice variant molecule, its polypeptide sequence, and the polynucleotides encoding the polypeptide sequence, and active fragments thereof. Also provided is a procedure for producing such polypeptides by recombinant techniques employing, for example, vectors and host cells. Also disclosed are methods for utilizing such polypeptides and modulators thereof for the treatment of diseases, including cancer, immune diseases, infectious diseases, and ischemic diseases.

摘要翻译： 本文公开了新鉴定的SDF-1剪接变体分子，其多肽序列和编码多肽序列的多核苷酸及其活性片段。 还提供了通过使用例如载体和宿主细胞的重组技术产生此类多肽的方法。 还公开了利用这些多肽及其调节剂治疗疾病的方法，包括癌症，免疫疾病，感染性疾病和缺血性疾病。

公开(公告)号：US08058403B2

公开(公告)日：2011-11-15

申请号：US12752936

申请日：2010-04-01

申请人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

发明人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

IPC分类号： C07K16/24

CPC分类号： C07K14/522 ,  Y10T436/143333

摘要： Disclosed herein is a newly identified SDF-1 splice variant molecule, its polypeptide sequence, and the polynucleotides encoding the polypeptide sequence, and active fragments thereof. Also provided is a procedure for producing such polypeptides by recombinant techniques employing, for example, vectors and host cells. Also disclosed are methods for utilizing such polypeptides and modulators thereof for the treatment of diseases, including cancer, immune diseases, infectious diseases, and ischemic diseases.

摘要翻译： 本文公开了新鉴定的SDF-1剪接变体分子，其多肽序列和编码多肽序列的多核苷酸及其活性片段。 还提供了通过使用例如载体和宿主细胞的重组技术产生此类多肽的方法。 还公开了利用这些多肽及其调节剂治疗疾病的方法，包括癌症，免疫疾病，感染性疾病和缺血性疾病。

公开(公告)号：US07776564B2

公开(公告)日：2010-08-17

申请号：US11587651

申请日：2005-04-29

申请人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

发明人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

IPC分类号： C07K14/52 ,  C12N5/10 ,  C12N15/19 ,  C12N15/63 ,  C12N15/64

CPC分类号： C07K14/522 ,  Y10T436/143333

摘要： Disclosed herein is a newly identified SDF-1 splice variant molecule, its polypeptide sequence, and the polynucleotides encoding the polypeptide sequence, and active fragments thereof. Also provided is a procedure for producing such polypeptides by recombinant techniques employing, for example, vectors and host cells. Also disclosed are methods for utilizing such polypeptides and modulators thereof for the treatment of diseases, including cancer, immune diseases, infectious diseases, and ischemic diseases.

摘要翻译： 本文公开了新鉴定的SDF-1剪接变体分子，其多肽序列和编码多肽序列的多核苷酸及其活性片段。 还提供了通过使用例如载体和宿主细胞的重组技术产生此类多肽的方法。 还公开了利用这些多肽及其调节剂治疗疾病的方法，包括癌症，免疫疾病，感染性疾病和缺血性疾病。

公开(公告)号：US06803194B1

公开(公告)日：2004-10-12

申请号：US09601970

申请日：2000-08-09

申请人： James G. Keck ,  Justin G. P. Wong

发明人： James G. Keck ,  Justin G. P. Wong

IPC分类号： C12Q168

CPC分类号： C12N15/113 ,  C12N15/1034 ,  C12N2310/111 ,  C12N2310/121 ,  C12N2799/021 ,  C12N2799/027

摘要： Double stranded DNAs, expression vectors and methods for their use are provided in which the intracellular expression of the double stranded DNAs is used to alter the phenotype of a target cell so that the function of a target nucleic acid that includes a nucleotide sequence encoding a motif of interest can be determined using a combinatorial ribozyme library. The members of the library are catalytic RNAs that disrupt the expression of the transcription product of the target nucleic acid. Disruption of transcription product expression results in an altered cell phenotype which is used to determine the function of the target nucleic acid. The specific phenotype or response may be associated with normal cellular processes, or it may contribute to the generation of pathogenesis involved in disease development. The compositions find use in high-throughput screens to assign gene functions. When associated with a pathogenic phenotype, these genes or their gene products can constitute therapeutic targets for treatment of diseases. The complete sequence of the gene containing the target nucleic acid need not to be known for the method to be used successfully.

摘要翻译： 提供双链DNA，表达载体及其使用方法，其中双链DNA的细胞内表达用于改变靶细胞的表型，使得靶核酸的功能包括编码基序的核苷酸序列 可以使用组合核酶库来确定感兴趣的。 文库的成员是破坏靶核酸转录产物表达的催化RNA。 转录产物表达的破坏导致改变的细胞表型，其用于确定靶核酸的功能。 特异性表型或反应可能与正常细胞过程相关，或可能有助于疾病发展中涉及的发病机制的产生。 该组合物可用于高通量筛选以分配基因功能。 当与致病表型相关时，这些基因或其基因产物可构成治疗疾病的治疗靶点。 含有目标核酸的基因的完整序列不必为成功使用的方法而知道。