公开(公告)号：US20120258071A1

公开(公告)日：2012-10-11

申请号：US13446254

申请日：2012-04-13

申请人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

发明人： Dirk Behrens ,  Elizabeth Bosch ,  Stephen K. Doberstein ,  Robert Forgan Halenbeck ,  Kevin Hestir ,  Min Mei Huang ,  Ernestine Lee ,  Haishan Lin ,  Thomas Linnemann ,  Shannon Marshall ,  Justin G. P. Wong ,  Ge Wu ,  Aileen Zhou ,  Cindy Leo ,  Lewis T. Williams

IPC分类号： A61K38/19 ,  C07K17/02 ,  A61P35/00 ,  A61P37/00 ,  A61P31/00 ,  C07K17/00 ,  C07K19/00

CPC分类号： C07K16/243 ,  A61K38/00 ,  C07K14/53

摘要： Disclosed is a newly identified secreted molecule, identified herein as “monocyte, granulocyte, and dendritic cell colony stimulating factor” (MGD-CSF), the polypeptide sequence, and polynucleotides encoding the polypeptide sequence. Also provided is a procedure for producing the polypeptide by recombinant techniques employing, for example, vectors and host cells. Additionally, procedures are described to modify the disclosed novel molecules of the invention to prepare fusion molecules. Also disclosed are methods for using the polypeptides and active fragments thereof for treatment of a variety of diseases, including, for example, cancer, autoimmune and inflammatory diseases, infectious diseases, and recurrent pregnancy loss.

摘要翻译： 公开了一种新鉴定的分泌型分子，本文鉴定为单核细胞，粒细胞和树突细胞集落刺激因子（MGD-CSF），多肽序列和编码多肽序列的多核苷酸。 还提供了通过使用例如载体和宿主细胞的重组技术产生多肽的方法。 另外，描述了修饰所公开的本发明新颖分子以制备融合分子的方法。 还公开了将多肽及其活性片段用于治疗各种疾病（包括例如癌症，自身免疫性和炎性疾病，感染性疾病和复发性妊娠损失）的方法。

公开(公告)号：US07795404B1

公开(公告)日：2010-09-14

申请号：US11985863

申请日：2007-11-15

申请人： Haishan Lin ,  Minmei Huang ,  Lewis T. Williams

发明人： Haishan Lin ,  Minmei Huang ,  Lewis T. Williams

IPC分类号： C07K14/435

CPC分类号： C07K14/705 ,  C07K14/47

摘要： Novel soluble Notch receptor ligands and polynucleotides encoding such are disclosed, as well as methods for producing and using these novel nucleotides and polypeptides. These novel polypeptides or agonists or antagonists thereto modulate cellular activity, regulate the growth and division of cell, including stem cells, progenitor and precursor cells, are involved in cell fate decisions, and provide prophylactic or therapeutic benefits for treatment of human diseases. Expression systems are provided that facilitate the production, secretion, and purification of these molecules.

摘要翻译： 公开了新型可溶性Notch受体配体和编码这些配体的多核苷酸，以及生产和使用这些新型核苷酸和多肽的方法。 这些新型多肽或其拮抗剂调节细胞活性，调节细胞（包括干细胞，祖细胞和前体细胞）的生长和分裂参与细胞命运决定，并且为治疗人类疾病提供预防或治疗益处。 提供了促进这些分子的生产，分泌和纯化的表达系统。

公开(公告)号：US07776564B2

公开(公告)日：2010-08-17

申请号：US11587651

申请日：2005-04-29

申请人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

发明人： Keting Chu ,  Lewis T. Williams ,  Justin G. P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

IPC分类号： C07K14/52 ,  C12N5/10 ,  C12N15/19 ,  C12N15/63 ,  C12N15/64

CPC分类号： C07K14/522 ,  Y10T436/143333

摘要： Disclosed herein is a newly identified SDF-1 splice variant molecule, its polypeptide sequence, and the polynucleotides encoding the polypeptide sequence, and active fragments thereof. Also provided is a procedure for producing such polypeptides by recombinant techniques employing, for example, vectors and host cells. Also disclosed are methods for utilizing such polypeptides and modulators thereof for the treatment of diseases, including cancer, immune diseases, infectious diseases, and ischemic diseases.

摘要翻译： 本文公开了新鉴定的SDF-1剪接变体分子，其多肽序列和编码多肽序列的多核苷酸及其活性片段。 还提供了通过使用例如载体和宿主细胞的重组技术产生此类多肽的方法。 还公开了利用这些多肽及其调节剂治疗疾病的方法，包括癌症，免疫疾病，感染性疾病和缺血性疾病。

公开(公告)号：US20100158911A1

公开(公告)日：2010-06-24

申请号：US12652720

申请日：2010-01-05

申请人： Lewis T. Williams ,  Elizabeth Bosch ,  Stephen Doberstein ,  Kevin Hestir ,  Diane Hollenbaugh ,  Ernestine Lee ,  Minmin Qin ,  Ali Sadra ,  Justin Wong ,  Ge Wu ,  Hongbing Zhang

发明人： Lewis T. Williams ,  Elizabeth Bosch ,  Stephen Doberstein ,  Kevin Hestir ,  Diane Hollenbaugh ,  Ernestine Lee ,  Minmin Qin ,  Ali Sadra ,  Justin Wong ,  Ge Wu ,  Hongbing Zhang

IPC分类号： A61K39/395 ,  C07K19/00 ,  C07K14/00 ,  C07H21/04 ,  A61K38/16 ,  A61P35/00 ,  A61P35/04 ,  C12N5/10 ,  C12P21/06 ,  C12N15/63 ,  G01N33/53

CPC分类号： C07K14/71 ,  A61K45/06 ,  C07K14/765 ,  C07K16/2863 ,  C07K17/08 ,  C07K2319/30 ,  C07K2319/32

摘要： The invention provides FGFR fusion proteins, methods of making them, and methods of using them to treat proliferative disorders, including cancers and disorders of angiogenesis. The FGFR fusion molecules can be made in CHO cells and may comprise deletion mutations in the extracellular domains of the FGFRs which improve their stability. These fusion proteins inhibit the growth and viability of cancer cells in vitro and in vivo. The combination of the relatively high affinity of these receptors for their ligand FGFs and the demonstrated ability of these decoy receptors to inhibit tumor growth is an indication of the clinical value of the compositions and methods provided herein.

摘要翻译： 本发明提供了FGFR融合蛋白，其制备方法以及使用它们来治疗增殖性疾病（包括癌症和血管生成障碍）的方法。 FGFR融合分子可以在CHO细胞中制备，并且可以包含改善其稳定性的FGFRs的细胞外结构域中的缺失突变。 这些融合蛋白体外和体内抑制癌细胞的生长和活力。 这些受体对其配体FGF的相对高亲和力的组合以及这些诱饵受体抑制肿瘤生长的证明的能力是本文提供的组合物和方法的临床价值的指示。

公开(公告)号：US20100062009A9

公开(公告)日：2010-03-11

申请号：US11587651

申请日：2005-04-29

申请人： Keting Chu ,  Lewis T. Williams ,  Justin G.P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

发明人： Keting Chu ,  Lewis T. Williams ,  Justin G.P. Wong ,  Kevin Hestir ,  Amy L. Tsui Collins ,  Ernestine Lee

IPC分类号： A61K39/00 ,  C12N15/11 ,  C07K14/00 ,  C12N15/87 ,  C12P21/04 ,  A61P31/00 ,  C12N5/06 ,  G01N33/566 ,  A61K38/00 ,  A61K35/12

CPC分类号： C07K14/522 ,  Y10T436/143333

摘要： Disclosed herein is a newly identified SDF-1 splice variant molecule, its polypeptide sequence, and the polynucleotides encoding the polypeptide sequence, and active fragments thereof. Also provided is a procedure for producing such polypeptides by recombinant techniques employing, for example, vectors and host cells. Also disclosed are methods for utilizing such polypeptides and modulators thereof for the treatment of diseases, including cancer, immune diseases, infectious diseases, and ischemic diseases.

摘要翻译： 本文公开了新鉴定的SDF-1剪接变体分子，其多肽序列和编码多肽序列的多核苷酸及其活性片段。 还提供了通过使用例如载体和宿主细胞的重组技术产生此类多肽的方法。 还公开了利用这些多肽及其调节剂治疗疾病的方法，包括癌症，免疫疾病，感染性疾病和缺血性疾病。

公开(公告)号：US20080112937A1

公开(公告)日：2008-05-15

申请号：US11632860

申请日：2005-07-19

申请人： Lewis T Williams ,  Hongbing Zhang ,  Srinivas Kothakota ,  Kristen Pierce ,  Pierre Alvaro Beaurang ,  Keting Chu

发明人： Lewis T Williams ,  Hongbing Zhang ,  Srinivas Kothakota ,  Kristen Pierce ,  Pierre Alvaro Beaurang ,  Keting Chu

IPC分类号： A61K48/00 ,  C12N5/08 ,  C12N15/09

CPC分类号： C12N5/0606 ,  A01K2217/05 ,  C12N15/873 ,  C12N2506/00

摘要： Aspects of the present invention relate to compositions and methods of reprogramming a somatic cell to give rise to an autologous embryonic stem cell. These methods involve providing a somatic cell of a donor subject, introducing the somatic cell into an embryo of a recipient subject to produce a chimeric embryo, allowing the chimeric embryo to develop further wherein the somatic cell will reprogram, and then selecting an autologous embryonic stem cell that has developed from the somatic cell. The methods and composition of producing pluripotent embryonic stem cells from a donor's own somatic cells invite the possibility of a number of therapeutic applications, including organ transplant and treatment of autoimmune diseases, cancer, and degenerative disorders such as diabetes, Alzheimer's, and Parkinson's.

摘要翻译： 本发明的方面涉及重编程体细胞以产生自体胚胎干细胞的组合物和方法。 这些方法涉及提供供体受试者的体细胞，将体细胞引入受体的胚胎以产生嵌合胚胎，允许嵌合胚胎进一步发育，其中体细胞将重新编程，然后选择自体胚胎干 从体细胞发育而来的细胞。 从供体自身体细胞细胞产生多能胚胎干细胞的方法和组成可引起许多治疗应用的可能性，包括器官移植和治疗自身免疫性疾病，癌症和退行性疾病如糖尿病，阿尔茨海默病和帕金森病。

公开(公告)号：US07105305B2

公开(公告)日：2006-09-12

申请号：US10027400

申请日：2001-12-19

申请人： Lewis T. Williams ,  Jaime A. Escobedo

发明人： Lewis T. Williams ,  Jaime A. Escobedo

IPC分类号： G01N33/53 ,  C07K14/49

CPC分类号： C07K14/71 ,  G01N33/74 ,  G01N2333/49

摘要： DNA sequences encoding human platelet-derived growth factor receptors (hPDGF-R), and expression constructs comprising sequences which encode a receptor that can be secreted or incorporated into the membrane of a mammalian cell. Peptide fragments with functions equivalent to the wild-type receptor, conferring a PDGF-sensitive mitogenic response on cells lacking the receptor are provided. The constructs can be used for enhancing PDGF response of cells, determining the regions involved in transducing the signal in response to PDGF binding, providing mutated analogs and evaluating drugs for their physiologic activity. Soluble fragments comprising PDGF receptor sequences are also provided, including important intracellular kinase insert sequences which interact with intracellular proteins.

公开(公告)号：US06974867B2

公开(公告)日：2005-12-13

申请号：US09870937

申请日：2001-05-30

申请人： Bin Wu ,  Todd W. Seeley ,  Lewis T. Williams

发明人： Bin Wu ,  Todd W. Seeley ,  Lewis T. Williams

IPC分类号： C12N15/09 ,  A61K31/7088 ,  A61K31/7105 ,  A61K38/00 ,  A61K38/55 ,  A61K39/395 ,  A61K48/00 ,  A61P35/00 ,  A61P43/00 ,  C12N9/12 ,  C12N9/99 ,  C12N15/113 ,  C07H21/04

CPC分类号： C12N15/1137 ,  A61K38/00 ,  C12N9/1205 ,  C12N2310/11 ,  C12Y207/01037

摘要： Inhibitors of KIAA0175 are provided that reduce the expression or biological activities of KIAA0175, p53 and/or p21 in a mammalian cell. KIAA0175 inhibitors include anti-sense molecules, ribozymes, antibodies and antibody fragments, proteins and polypeptides as well as small molecules. KIAA0175 inhibitors find use in compositions and methods for decreasing KIAA0175, p53 and/or p21 gene expression as well as methods for increasing the chemo and/or radiosensitivity of mammalian cells, including tumor cells, methods for decreasing the side effects of cancer therapy and methods for treating neoplastic diseases.

摘要翻译： 提供KIAA0175的抑制剂，其减少哺乳动物细胞中KIAA0175，p53和/或p21的表达或生物学活性。 KIAA0175抑制剂包括反义分子，核酶，抗体和抗体片段，蛋白质和多肽以及小分子。 KIAA0175抑制剂用于减少KIAA0175，p53和/或p21基因表达的组合物和方法，以及用于增加哺乳动物细胞（包括肿瘤细胞）的化学和/或放射敏感性的方法，用于降低癌症治疗的副作用的方法和方法 用于治疗肿瘤性疾病。

公开(公告)号：US06964868B1

公开(公告)日：2005-11-15

申请号：US09297648

申请日：1999-01-28

申请人： Lewis T. Williams ,  Jaime Escobedo ,  Michael A. Innis ,  Pablo Dominguez Garcia ,  Julie Sudduth-Klinger ,  Christoph Reinhard ,  Klaus Giese ,  Filippo Randazzo ,  Giulia C. Kennedy ,  David Pot ,  Altaf Kassam ,  George Lamson ,  Radoje Drmanac ,  Radomir Crkvenjakov ,  Mark Dickson ,  Snezana Drmanac ,  Ivan Labat ,  Dena Leshkowitz ,  David Kita ,  Veronica Garcia ,  Lee William Jones ,  Birgit Stache-Crain

发明人： Lewis T. Williams ,  Jaime Escobedo ,  Michael A. Innis ,  Pablo Dominguez Garcia ,  Julie Sudduth-Klinger ,  Christoph Reinhard ,  Klaus Giese ,  Filippo Randazzo ,  Giulia C. Kennedy ,  David Pot ,  Altaf Kassam ,  George Lamson ,  Radoje Drmanac ,  Radomir Crkvenjakov ,  Mark Dickson ,  Snezana Drmanac ,  Ivan Labat ,  Dena Leshkowitz ,  David Kita ,  Veronica Garcia ,  Lee William Jones ,  Birgit Stache-Crain

IPC分类号： C07K14/47 ,  C12N1/21 ,  C12N5/10 ,  C12N15/12 ,  C12N15/63

CPC分类号： C07K14/4748

摘要： This invention relates to novel human polynucleotides and variants thereof, their encoded polypeptides and variants thereof, to genes corresponding to these polynucleotides and to proteins expressed by the genes. The invention also relates to diagnostic and therapeutic agents employing such novel human polynucleotides, their corresponding genes or gene products, e.g., these genes and proteins, including probes, antisense constructs, and antibodies.

摘要翻译： 本发明涉及对应于这些多核苷酸的基因和由该基因表达的蛋白质的新型人多核苷酸及其变体，其编码的多肽及其变体。 本发明还涉及使用这种新型人多核苷酸的诊断和治疗剂，其相应的基因或基因产物，例如这些基因和蛋白质，包括探针，反义构建体和抗体。

公开(公告)号：US06475782B1

公开(公告)日：2002-11-05

申请号：US08451693

申请日：1995-05-26

申请人： Jaime A. Escobedo ,  Lewis T. Williams

发明人： Jaime A. Escobedo ,  Lewis T. Williams

IPC分类号： C12N510

CPC分类号： C07K14/71

摘要： A DNA sequence encoding human platelet-derived growth factor receptor (hPDGF-R) has now been isolated and sequenced. An expression construct comprising the sequence encodes a receptor that can be secreted or incorporated into the membrane of a mammalian cell. The incorporated receptor is functionally equivalent to the wild-type receptor, conferring a PDGF-sensitive mitogenic response on cells lacking the receptor. The construct can be used for enhancing PDGF response of cells, determining the regions involved in transducing the signal in response to PDGF binding, providing mutated analogs and evaluating drugs for their physiologic activity.

摘要翻译： 编码人血小板衍生生长因子受体（hPDGF-R）的DNA序列现已被分离并测序。 包含该序列的表达构建体编码可分泌或并入哺乳动物细胞膜的受体。 所掺入的受体在功能上等同于野生型受体，赋予对缺乏受体的细胞的PDGF敏感的促有丝分裂反应。 该构建体可以用于增强细胞的PDGF应答，确定响应于PDGF结合参与转导信号的区域，提供突变的类似物并评价药物的生理活性。