公开(公告)号：US10287314B2

公开(公告)日：2019-05-14

申请号：US14747029

申请日：2015-06-23

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: B01D15/36 ,  B01D15/12 ,  B01D15/18 ,  B01D15/38 ,  B01J39/26 ,  B01J41/20 ,  C07K1/36 ,  C07K1/16 ,  B01J20/282 ,  C07K1/20 ,  B01J43/00 ,  B01J47/04 ,  C07K16/00 ,  B01J20/20

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

公开(公告)号：US11634457B2

公开(公告)日：2023-04-25

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: B01D15/36 ,  C07K1/36 ,  C07K1/16 ,  B01D15/18 ,  C07K1/18 ,  C07K1/22 ,  C07K16/00 ,  B01J20/282 ,  C07K1/20 ,  B01D15/12 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  B01J20/20 ,  B01D15/38

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US20190218250A1

公开(公告)日：2019-07-18

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: C07K1/36 ,  B01J20/20 ,  B01D15/12 ,  B01D15/36 ,  C07K16/00 ,  B01J47/04 ,  B01J43/00 ,  B01J41/20 ,  B01J39/26 ,  C07K1/20 ,  C07K1/16 ,  B01J20/282

CPC classification number: C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US20160016992A1

公开(公告)日：2016-01-21

申请号：US14747029

申请日：2015-06-23

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: C07K1/36 ,  B01J41/20 ,  B01J39/26 ,  B01J20/20

CPC classification number: C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

Abstract translation: 本发明提供新颖和改进的蛋白质纯化方法，其包含某些类型的碳质材料，并且导致有效和选择性地除去某些不需要的杂质，而不会不利地影响所需蛋白质产物的产率。

公开(公告)号：US20150258468A1

公开(公告)日：2015-09-17

申请号：US14728496

申请日：2015-06-02

Applicant: EMD Millipore Corporation

Inventor： Martin Siwak

IPC: B01D15/12 ,  B01D15/38

CPC classification number: B01D15/125 ,  B01D15/3804 ,  B01D15/3809 ,  B01D29/41 ,  B01D39/083 ,  B01D39/086 ,  B01D39/1607 ,  B01D39/18 ,  B01D39/20 ,  B01D61/142 ,  B01D61/16 ,  B01D69/141 ,  B01D2239/0407 ,  B01D2239/0428 ,  B01D2239/065 ,  B01D2239/0654 ,  B01D2239/0695 ,  B01D2311/04 ,  B01J20/28014 ,  B01J20/28028 ,  C12P21/00 ,  B01D2311/2649 ,  B01D2311/2626

Abstract: The present invention is to a prefilter for affinity chromatography columns. The prefilter, positioned upstream of the column inlet, reduces the presence of non-specific binding (NSB) species that enter the system, thereby extending the yield, capacity and lifetime of the column. Suitable agents include but are not limited to hydrophobic entities; lipophilic entities; activated carbon; charged cation or anion entities; ligands; particles such as fumed silica, glass, controlled pore glass or derivitized version of each; silica or silicates; and combinations thereof. The material(s) can be incorporated into a variety of media such as fibers, beads, membranes and the like and then incorporated into a variety of device designs including lenticular pads, depth filters, bead containing columns, spiral wound devices, TFF devices and the like.

Abstract translation: 本发明涉及亲和层析柱的预过滤器。 位于柱入口上游的预过滤器减少进入系统的非特异性结合（NSB）物质的存在，从而延长柱的产率，容量和寿命。 合适的试剂包括但不限于疏水性实体; 亲脂性实体; 活性炭; 带电阳离子或阴离子实体; 配体; 颗粒如煅制二氧化硅，玻璃，受控孔玻璃或每种的衍生版本; 二氧化硅或硅酸盐; 及其组合。 这些材料可以结合到各种介质如纤维，珠粒，膜等中，然后结合到各种装置设计中，包括透镜衬垫，深度过滤器，包含珠的柱，螺旋缠绕装置，TFF装置和 类似。