公开(公告)号：US20220334111A1

公开(公告)日：2022-10-20

申请号：US17852053

申请日：2022-06-28

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Junichi KATADA ,  Atsuhiko WADA ,  Dai UJIHARA

IPC: G01N33/543 ,  G01N33/532 ,  G01N33/553

Abstract: An immunochromatography includes steps of mixing a specimen capable of containing an antigen and a modified particle, which is a particle modified with a substance having a specific affinity to the antigen, to obtain a mixture containing particle composite bodies; sedimenting the particle composite bodies in the mixture using a centrifuge; dissociating the sedimented particle composite bodies into the particles and the antigen by mixing the sedimented particle composite bodies with a dissociation solution, recovering an antigen-concentrated solution by sedimenting the dissociated particles using a centrifuge; neutralizing the antigen-concentrated solution using a neutralization solution; spreading particle composite bodies for labeling on an insoluble carrier having a reaction site, in a state where the particle composite bodies for labeling, which are composite bodies of the antigen in the neutralized antigen-concentrated solution and a modified particle for labeling, are formed; and capturing the particle composite bodies for labeling at the reaction site.

公开(公告)号：US20220341926A1

公开(公告)日：2022-10-27

申请号：US17859292

申请日：2022-07-07

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Junichi KATADA

IPC: G01N33/543

Abstract: An immunological test method includes a concentration step of mixing a solution capable of containing an antigen, a superabsorbent polymer, and a labeled antibody against the antigen, to obtain a concentrated and mixed solution which is a concentrated mixed solution containing composite bodies of the antigen and the labeled antibody and a detection step of detecting the composite bodies in the concentrated and mixed solution using an antigen-antibody reaction, in which a swelling ratio of the superabsorbent polymer is more than 0.2 g/g and less than 800 g/g.

公开(公告)号：US20130150562A1

公开(公告)日：2013-06-13

申请号：US13759463

申请日：2013-02-05

Applicant: FUJIFILM CORPORATION

Inventor： Morihito IKEDA ,  Taisei NISHIMI ,  Junichi KATADA

IPC: C07F7/18

CPC classification number: C07F7/1804 ,  G01N33/54353 ,  G01N33/54393 ,  Y10T428/2991

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract translation: 一种用于将生理活性物质固定在固相载体上的固定方法，所述方法包括：使固相载体与由下式（I）表示的含酸酐官能团的硅烷偶联剂接触; 并且在0℃至60℃的范围内的温度下，将生理活性物质与酸酐官能团结合，同时保持接触后的固相载体; 提供生理活性物质固定化载体和用于固定的载体。 此外，包括用下述通式（I）表示的含酸酐官能团的硅烷偶联剂处理的多孔材料的载体，固定在多孔材料上的封端剂; 并提供其制造方法。

公开(公告)号：US20130137190A1

公开(公告)日：2013-05-30

申请号：US13749148

申请日：2013-01-24

Applicant: FUJIFILM Corporation

Inventor： Junichi KATADA ,  Hideyuki KARAKI ,  Takayoshi OYAMADA

IPC: G01N33/543

CPC classification number: G01N33/543 ,  G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/81

Abstract: The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.

公开(公告)号：US20230341398A1

公开(公告)日：2023-10-26

申请号：US18318892

申请日：2023-05-17

Applicant: FUJIFILM Corporation ,  PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY

Inventor： Dai UJIHARA ,  Junichi KATADA ,  Akihide RYO

IPC: G01N33/569 ,  G01N33/553

CPC classification number: G01N33/56983 ,  G01N33/553 ,  G01N2333/165

Abstract: An object of the present invention is to provide a SARS-CoV-2 detection kit and a SARS-CoV-2 detection method which make it possible to simply detect SARS-CoV-2 with higher sensitivity. According to the present invention, there is provided a SARS-CoV-2 detection kit which is for specifically detecting a nucleocapsid protein contained in a biological specimen and contains at least one antibody that specifically reacts with a SARS-CoV-2 nucleocapsid protein (NP), the SARS-CoV-2 detection kit including a first container that houses a silver-containing compound, and a second container that houses a reducing agent capable of reducing silver ions, in which the antibody includes at least one antibody that belongs to a subclass IgG2b.

公开(公告)号：US20230251248A1

公开(公告)日：2023-08-10

申请号：US18182227

申请日：2023-03-10

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Junya YOSHIDA ,  Hiroyasu ISHII ,  Junichi KATADA

IPC: G01N33/543 ,  B01D69/02 ,  B01D67/00

CPC classification number: G01N33/543 ,  B01D69/02 ,  B01D67/0079

Abstract: There are provided a concentration device that makes it possible to concentrate a sample solution in a short time, a sample solution concentration method using the concentration device to concentrate a sample solution, a sample solution concentration method using the sample solution examination method, and an examination kit including the concentration device and the detection device. The concentration device for concentrating a sample solution which is an aqueous solution containing a high-molecular-weight molecule contained in a biological fluid is a concentration device including a first container containing a super absorbent polymer and a second container, in which a part of the first container is constituted of a discharge unit consisting of a porous membrane having a hole diameter of 0.05 μm or more and 10 μm or less, the super absorbent polymer absorbs a solution contained in the sample solution injected into the first container to generate, in the first container, a sample solution concentrated solution which is a concentrated solution of the sample solution, and the second container recovers the sample solution concentrated solution discharged from the discharge unit.

公开(公告)号：US20220196643A1

公开(公告)日：2022-06-23

申请号：US17690101

申请日：2022-03-09

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Naoyuki NISHIKAWA ,  Junichi KATADA ,  Atsuhiko WADA ,  Dai UJIHARA

IPC: G01N33/543

Abstract: The immunological test method includes a concentration step of concentrating an antigen-containable solution by mixing the antigen-containable solution with a superabsorbent polymer to obtain an antigen-concentrated solution, and a detection step of detecting an antigen in the antigen-concentrated solution using an antigen-antibody reaction, in which a swelling ratio of the superabsorbent polymer is more than 0.2 g/g and less than 800 g/g, and an antibody that is used in the antigen-antibody reaction is a monoclonal antibody.

公开(公告)号：US20220196523A1

公开(公告)日：2022-06-23

申请号：US17690854

申请日：2022-03-09

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Naoyuki NISHIKAWA ,  Junichi KATADA ,  Atsuhiko WADA ,  Dai UJIHARA

IPC: G01N1/40 ,  G01N33/543

Abstract: An immunochromatography including steps of mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a substance having a specific affinity to the antigen; collecting the magnetic particles using magnetism; dissociating the modified magnetic particles to obtain an antigen-concentrated solution by mixing the collected magnetic particles with a dissociation solution, an amount of which is smaller than the antigen-containable specimen; obtaining a neutralized antigen-concentrated solution; spreading gold particle composite bodies on an insoluble carrier having a reaction site at which a second binding substance has been immobilized, in a state where the gold particle composite bodies which are composite bodies of an antigen in the neutralized antigen-concentrated solution and modified gold particles which are gold particles modified with a first binding substance are formed; capturing the gold particle composite bodies at the reaction site; and silver-amplifying the gold particle composite body.

公开(公告)号：US20230273204A1

公开(公告)日：2023-08-31

申请号：US18156214

申请日：2023-01-18

Applicant: FUJIFILM Corporation

Inventor： Tomoya OHARA ,  Natsuki OYABU ,  Atsuhiko WADA ,  Junichi KATADA ,  Dai UJIHARA

IPC: G01N33/569 ,  C07K16/12 ,  G01N33/548 ,  A61K39/04

CPC classification number: G01N33/5695 ,  C07K16/1289 ,  G01N33/548 ,  A61K39/04 ,  G01N2333/35 ,  G01N2400/00 ,  G01N2405/00 ,  G01N2469/10

Abstract: An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detecting Mycobacterium tuberculosis with high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detecting Mycobacterium tuberculosis is provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

公开(公告)号：US20220326227A1

公开(公告)日：2022-10-13

申请号：US17851215

申请日：2022-06-28

Applicant: FUJIFILM Corporation

Inventor： Yoshihiro ABURAYA ,  Junichi KATADA

IPC: G01N33/531 ,  G01N33/543

Abstract: An immunochromatography includes a concentration step of concentrating a solution capable of containing an antigen by ultrafiltration to obtain an antigen-concentrated solution; a spreading step of spreading particle composite bodies on an insoluble carrier having a reaction site at which a second binding substance capable of binding to an antigen in the antigen-concentrated solution has been immobilized, in a state where the particle composite bodies which are composite bodies of the antigen and a modified particle which is a particle modified with a first binding substance capable of binding to the antigen are formed; a capturing step of capturing the particle composite bodies at the reaction site of the insoluble carrier; and an amplification step of amplifying information of the particle composite bodies captured in the capturing step.