公开(公告)号：US20240279733A1

公开(公告)日：2024-08-22

申请号：US18567989

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Fei Shen ,  Mathieu Lessard-Viger ,  Eric Brustad ,  Allison Meade ,  Esteban Armijo ,  Michael Howard ,  Jeffrey Fisher ,  Jonathan Boutell ,  Ramon Saracho ,  Olivia Ghazinejad ,  Seth McDonald ,  Lena Storms ,  Jeffrey Brodin

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: The present disclosure is directed to decoupling library capture (template seeding) from cluster generation to optimise both processes. This is achieved by introducing orthogonality between the seeding and clustering primer.

公开(公告)号：US20250084477A1

公开(公告)日：2025-03-13

申请号：US18575479

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Esteban Armijo ,  Jonathan Mark Boutell ,  Jeffrey Brodin ,  Eli M. Carrami ,  Maria Candelaria Rogert Bacigalupo ,  Randall Smith ,  Mathieu Lessard-Viger ,  Ludovic Vincent

IPC: C12Q1/6876

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

公开(公告)号：US20240011080A1

公开(公告)日：2024-01-11

申请号：US18000663

申请日：2021-12-20

Applicant: Illumina, Inc.

Inventor： Hayden Black ,  Esteban Armijo ,  Mathieu Lessard-Viger

IPC: C12Q1/6837

CPC classification number: C12Q1/6837

Abstract: A composition for capturing target polynucleotides at a surface of a substrate is provided. The composition may include a plurality of capture primers coupled to the surface of the substrate and including modified nucleic acids; and a plurality of orthogonal capture primers coupled to the surface of the substrate and including modified nucleic acids. The modified nucleic acids of the capture primers may include locked nucleic acid (LNA), peptide nucleic acid (PNA), or super T. The modified nucleic acids of the orthogonal capture primers may include locked nucleic acid (LNA), peptide nucleic acid (PNA), or super T.