公开(公告)号：US20180327832A1

公开(公告)日：2018-11-15

申请号：US15969562

申请日：2018-05-02

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Sean M. Ramirez ,  Brian D. Mather ,  Edwin Li ,  Sojeong Moon ,  Innsu Daniel Kim ,  Alexandre Richez ,  Ludovic Vincent ,  Xavier von Hatten ,  Hai Quang Tran ,  Maxwell Zimmerley ,  Julia Morrison ,  Gianluca Andrea Artioli ,  Krystal Sly ,  Hayden Black ,  Lewis J. Kraft ,  Hong Xie ,  Wei Wei ,  Ryan Sanford

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806

Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

公开(公告)号：US20230272469A1

公开(公告)日：2023-08-31

申请号：US18195321

申请日：2023-05-09

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Sean M. Ramirez ,  Brian D. Mather ,  Edwin Li ,  Sojeong Moon ,  Innsu Daniel Kim ,  Alexandre Richez ,  Ludovic Vincent ,  Xavier von Hatten ,  Hai Quang Tran ,  Maxwell Zimmerley ,  Julia Morrison ,  Gianluca Andrea Artioli ,  Krystal Sly ,  Hayden Black ,  Lewis J. Kraft ,  Hong Xie ,  Wei Wei ,  Ryan Sanford

IPC: C12Q1/6874 ,  C12Q1/6806 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  B01L3/502707 ,  B01L2300/165 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/0877

Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

公开(公告)号：US11384392B2

公开(公告)日：2022-07-12

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210208055A1

公开(公告)日：2021-07-08

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20210024991A1

公开(公告)日：2021-01-28

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  G03F7/075 ,  C08G77/04 ,  C08F220/56

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20250084477A1

公开(公告)日：2025-03-13

申请号：US18575479

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Esteban Armijo ,  Jonathan Mark Boutell ,  Jeffrey Brodin ,  Eli M. Carrami ,  Maria Candelaria Rogert Bacigalupo ,  Randall Smith ,  Mathieu Lessard-Viger ,  Ludovic Vincent

IPC: C12Q1/6876

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

公开(公告)号：US20240302738A1

公开(公告)日：2024-09-12

申请号：US18667993

申请日：2024-05-17

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: G03F7/00 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/004 ,  G03F7/075 ,  G03F7/16

CPC classification number: G03F7/0002 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11988957B2

公开(公告)日：2024-05-21

申请号：US18317865

申请日：2023-05-15

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: G03F7/00 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/075 ,  G03F7/16 ,  G03F7/004

CPC classification number: G03F7/0002 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11846580B2

公开(公告)日：2023-12-19

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  G01N15/10 ,  B01J19/00

CPC classification number: G01N15/1436 ,  B01J19/0046 ,  B01L3/502 ,  B01L3/502707 ,  C09D133/24 ,  C09D133/26 ,  C12Q1/6874 ,  G01N15/1484 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00418 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00722 ,  B01L2200/12 ,  B01L2300/087 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/0887 ,  B01L2300/16 ,  G01N2015/1006

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20230332224A1

公开(公告)日：2023-10-19

申请号：US18317865

申请日：2023-05-15

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.