公开(公告)号：US20240209419A1

公开(公告)日：2024-06-27

申请号：US18539988

申请日：2023-12-14

Applicant: Illumina, Inc.

Inventor： Naneki McCallum ,  Fei Shen ,  Xiaoyu Ma ,  Weixian Xi ,  Olivia Ghazinejad ,  Jeffrey Brodin ,  Jeff Fisher

IPC: C12Q1/6818 ,  C12Q1/6874

CPC classification number: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6851

Abstract: Methods for on-flow cell selective capture and enrichment of clustered beads, general capture strategies on bead mobility on flow cell surfaces, sorting clustered and unclustered beads, and flow cell reusability for bead immobilization onto flow cells.

公开(公告)号：US20240271126A1

公开(公告)日：2024-08-15

申请号：US18562095

申请日：2022-05-26

Applicant: ILLUMINA, INC.

Inventor： Niall Anthony Gormley ,  Carlo Randise-Hinchliff ,  Jeffrey Brodin ,  Esther Musgrave-Brown ,  Sarah E. Shultzaberger ,  Andrew Slatter ,  Jeffrey S. Fisher

IPC: C12N15/10 ,  C12N15/11 ,  C12Q1/48

CPC classification number: C12N15/1096 ,  C12N15/11 ,  C12Q1/485

Abstract: Nucleic acid techniques are disclosed. Embodiments include modified nucleotides with oligonucleotide adapters that are coupled via cleavable linkers. Incorporation of the modified nucleotide at a 3′ end of a nucleic acid permits end-adapterization via ligation of a free 5′ end of the oligonucleotide adapter to a 3′ reactive group of the modified nucleotide and cleavage at the cleavable linker to liberate a free 3′ end.

公开(公告)号：US20240279733A1

公开(公告)日：2024-08-22

申请号：US18567989

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Fei Shen ,  Mathieu Lessard-Viger ,  Eric Brustad ,  Allison Meade ,  Esteban Armijo ,  Michael Howard ,  Jeffrey Fisher ,  Jonathan Boutell ,  Ramon Saracho ,  Olivia Ghazinejad ,  Seth McDonald ,  Lena Storms ,  Jeffrey Brodin

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: The present disclosure is directed to decoupling library capture (template seeding) from cluster generation to optimise both processes. This is achieved by introducing orthogonality between the seeding and clustering primer.

公开(公告)号：US20250084477A1

公开(公告)日：2025-03-13

申请号：US18575479

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Esteban Armijo ,  Jonathan Mark Boutell ,  Jeffrey Brodin ,  Eli M. Carrami ,  Maria Candelaria Rogert Bacigalupo ,  Randall Smith ,  Mathieu Lessard-Viger ,  Ludovic Vincent

IPC: C12Q1/6876

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

公开(公告)号：US20250109428A1

公开(公告)日：2025-04-03

申请号：US18899460

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Olivia Ghazinejad ,  Fei Shen ,  Kyle Bentz ,  Rohit Subramanian ,  Eric Brustad ,  Lena Storms ,  Xiaoyu Ma ,  Mathieu Lessard-Viger ,  Jeffrey Brodin

IPC: C12Q1/6806 ,  B01L3/00 ,  C12Q1/6844

Abstract: In some examples, a hybrid particle for use in capturing a polynucleotide may include a scaffold molecule including a seeding primer and a plurality of first moieties, and a nanoparticle including a plurality of second moieties. The scaffold molecule is coupled to the nanoparticle via interactions between the plurality of first moieties and the plurality of second moieties.

公开(公告)号：US20240409981A1

公开(公告)日：2024-12-12

申请号：US18569421

申请日：2022-07-21

Applicant: Illumina, Inc.

Inventor： Anurag Agrawal ,  Jeffrey Brodin ,  Jeffrey Fisher ,  Bo Lu ,  Chenlu Pan

IPC: C12Q1/6818 ,  C12Q1/6827

Abstract: Some examples relate to a method for characterizing polynucleotides in a sample. First polynucleotides coupled to a first substrate may be hybridized to second polynucleotides in a sample. First labeled nucleotides may be added to the first polynucleotides using a sequence of the second polynucleotides. A first signal intensity from the first labeled nucleotides is measured. Second labeled nucleotides are added to the first polynucleotides using the sequence of the second polynucleotides. A second signal intensity from the second labeled nucleotides is measured. The first signal intensity is normalized using the second signal intensity. The normalized first signal intensity characterizes the second polynucleotides in the sample.

公开(公告)号：US20230287476A1

公开(公告)日：2023-09-14

申请号：US18151128

申请日：2023-01-06

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Elisabet Rosàs-Canyelles ,  Fei Shen ,  Jeffrey Brodin ,  Lena Storms ,  Jeffrey S. Fisher

IPC: C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

CPC classification number: C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

Abstract: Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3′ portion comprising a transposon end sequence and a tag, and the second polynucleotide comprises a 5′ portion that is complementary to and hybridized to the transposon end sequence, and wherein the polyester bead has a melting point of from 50° C. to 65° C. Flow cells and methods related to these polyester beads are described. Also described herein are compositions comprising a bead and at least one nanoparticle and methods of use of such compositions comprising transposome complexes immobilized to nanoparticles.