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公开(公告)号：US20250084477A1
公开(公告)日：2025-03-13
申请号：US18575479
申请日：2022-11-01
Applicant: ILLUMINA, INC.
Inventor： Esteban Armijo , Jonathan Mark Boutell , Jeffrey Brodin , Eli M. Carrami , Maria Candelaria Rogert Bacigalupo , Randall Smith , Mathieu Lessard-Viger , Ludovic Vincent
IPC: C12Q1/6876
Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.
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2.发明公开公开(公告)号：US20230407388A1
公开(公告)日：2023-12-21
申请号：US18303905
申请日：2023-04-20
Applicant: Illumina, Inc. , Illumina Cambridge Limited
Inventor： Tarun Khurana , Yir-Shyuan Wu , Niall Anthony Gormley , Jonathan Mark Boutell
IPC: C12Q1/6874
CPC classification number: C12Q1/6874
Abstract: Described herein is a polynucleotide for use as a sequencing template comprising multiple inserts. Also described herein are method of generating and using these polynucleotides and methods of use of such templates, including analysis of contiguity information. Further, sequencing templates comprising an insert sequence and a copy of the insert sequence can be used to correct for random errors generated during sequencing or amplification or to identify nucleobase damage or other mutation that leads to non-canonical base pairing in a double-stranded nucleic acid. Methods of performing methylation analysis are also described herein.
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公开(公告)号：US11827932B2
公开(公告)日：2023-11-28
申请号：US17153309
申请日：2021-01-20
Applicant: Illumina, Inc.
Inventor： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6869
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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公开(公告)号：US20170335380A1
公开(公告)日：2017-11-23
申请号：US15672161
申请日：2017-08-08
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US20170022559A1
公开(公告)日：2017-01-26
申请号：US15207892
申请日：2016-07-12
Applicant: Illumina, Inc.
Inventor： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
Abstract translation: 本公开提供了用于检测样品中多个不同核苷酸的方法和系统。 具体地，本公开提供了使用比检测到的核苷酸数目少于检测到的核苷酸数目和/或比检测到的核苷酸数目更少的成像事件来检测样品中的多个不同核苷酸。
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Patent Agency Ranking
6.发明公开公开(公告)号：US20240117416A1
公开(公告)日：2024-04-11
申请号：US18274974
申请日：2022-01-28
Applicant: Illumina, Inc. , Illumina Cambridge Limited
Inventor： Yir-Shyuan Wu , Filiz Gorpe-Yasar , Tarun Kuman Khurana , Jonathan Mark Boutell
IPC: C12Q1/6834 , C12Q1/6844 , C12Q1/6874
CPC classification number: C12Q1/6834 , C12Q1/6844 , C12Q1/6874
Abstract: The disclosure relates to methods, compositions, and kits for improving seeding efficiency of flow cells with polynucleotides, and applications thereof, including for sequencing.
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公开(公告)号：US11938475B2
公开(公告)日：2024-03-26
申请号：US17114134
申请日：2020-12-07
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Hongji Ren , Jonathan Mark Boutell , John A. Moon , M. Shane Bowen , Alex Nemiroski , Gary Mark Skinner , Kenny Chen
IPC: B01L3/00 , B01J19/00 , C08F220/56 , C08F220/60 , C09D133/26 , C12Q1/6876
CPC classification number: B01L3/502 , B01J19/0046 , C08F220/56 , C08F220/603 , C09D133/26 , C12Q1/6876 , B01J2219/00475 , B01J2219/00729 , B01L2200/12 , B01L2300/0893 , B01L2300/16
Abstract: In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
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公开(公告)号：US11795581B2
公开(公告)日：2023-10-24
申请号：US16158120
申请日：2018-10-11
Applicant: Illumina, Inc.
Inventor： Molly He , Michael Previte , Misha Golynskiy , Matthew William Kellinger , Sergio Peisajovich , Jonathan Mark Boutell
CPC classification number: C40B50/16 , B01J19/0046 , B01L3/5085 , B01L3/54 , C12N15/1062 , C12N15/1065 , C12N15/1068 , C12N15/1075 , C40B20/04 , B01J2219/005 , B01J2219/00317 , B01J2219/00459 , B01J2219/00527 , B01J2219/00547 , B01J2219/00572 , B01J2219/00626 , B01J2219/00743 , B01L2300/021 , C12N15/1065 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1062 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1075 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1068 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515
Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.
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公开(公告)号：US20220243261A1
公开(公告)日：2022-08-04
申请号：US17575279
申请日：2022-01-13
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/6848 , B01J19/00 , C12Q1/6844 , C12Q1/6874
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US20210139978A1
公开(公告)日：2021-05-13
申请号：US17153309
申请日：2021-01-20
Applicant: Illumina, Inc.
Inventor： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC: C12Q1/6874 , C12Q1/6869
Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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