公开(公告)号：US09790463B2

公开(公告)日：2017-10-17

申请号：US14229596

申请日：2014-03-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Tai-Rong Guo ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Wan-Ju Yu ,  Chih-Hsi Liu

IPC: C12N1/20

CPC classification number: C12N1/20

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.

公开(公告)号：US08968687B2

公开(公告)日：2015-03-03

申请号：US14143268

申请日：2013-12-30

Applicant: Industrial Technology Research Institute

Inventor： Yin-Lung Han ,  Tai-Rong Guo ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Wan-Ju Yu

IPC: C22B3/00 ,  C07K14/00 ,  C02F3/00 ,  C12N1/02 ,  C12N1/20 ,  C22B7/00 ,  C07K14/195 ,  C02F5/08 ,  C12R1/01 ,  C12P3/00

CPC classification number: C22B7/006 ,  C02F1/286 ,  C02F3/34 ,  C02F5/08 ,  C02F2101/20 ,  C02F2301/106 ,  C02F2305/00 ,  C07K14/195 ,  C12P3/00 ,  C12R1/01 ,  C22B11/04 ,  C22B11/042 ,  Y02P10/238

Abstract: A method for recycling metals is provided by using extracellular proteins excreted by a specific thermophilic bacteria strain, Tepidimonas fonticaldi sp. nov., in which the extracellular proteins show excellent metal-ion binding ability, being useful in recycling rare earth metal ions and precious metal ions from geothermal fluids, boiler solutions, industrial wastewater or hard water.

Abstract translation: 通过使用由特异性嗜热细菌菌株Tepidimonas fonticaldi sp。分泌的细胞外蛋白质来提供回收金属的方法。 其中细胞外蛋白质显示出优异的金属离子结合能力，可用于从地热液体，锅炉溶液，工业废水或硬水中回收稀土金属离子和贵金属离子。

公开(公告)号：US10196600B2

公开(公告)日：2019-02-05

申请号：US14736363

申请日：2015-06-11

Applicant: Industrial Technology Research Institute

Inventor： Ming-Der Bai ,  Chung-Cheng Han ,  Wen-Chang Lu ,  Hou-Peng Wan ,  Jo-Shu Chang ,  Chun-Yen Chen ,  Hsin-Yueh Chang

IPC: C12N1/06 ,  C12N1/20 ,  C12P1/04 ,  C12R1/07

Abstract: The disclosure provides a method for manufacturing an active substance for inducing self-lysis in microalga cells, including: inoculating a bacterial strain belonging to Bacillus into a culturing medium to obtain a bacterial suspension; culturing the bacterial strain belonging to Bacillus at least to a stationary phase to a condition in which the bacterial strain belonging to Bacillus aggregates in the bacterial suspension and the bacterial suspension becomes pellucid; and after the bacterial strain belonging to Bacillus aggregates in the bacterial suspension and the bacterial suspension becomes pellucid, performing a vacuum distillation procedure on the bacterial suspension to obtain an active solution, wherein the active solution contains an active substance for inducing self-lysis in microalga cells.

公开(公告)号：US09580738B2

公开(公告)日：2017-02-28

申请号：US14838612

申请日：2015-08-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Chih-Hsi Liu

IPC: C12P21/00

CPC classification number: C12P21/00

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract translation: 本发明提供了从Tepidimonas属产生细胞外蛋白的方法，包括：用培养基对培养培养基进行发酵培养以形成发酵液，其中培养基的组成包括作为有机物的碳源 酸，选自乙酸酯，乳酸和丁酸酯; 选自（NH 4）2 SO 4，NH 4 NO 3，NH 4 Cl和尿素的氮源; 磷酸盐 碳酸盐 MgCl2; 酵母抽提物; 和微量元素，其中发酵培养的气体进料速率为约0-0.1vvm; 在发酵培养完成后，收集发酵液，其中发酵液含有从Tepidimonas属细菌分泌的细胞外蛋白质。

公开(公告)号：US08828238B2

公开(公告)日：2014-09-09

申请号：US13846224

申请日：2013-03-18

Applicant: Industrial Technology Research Institute

Inventor： Yin-Lung Han ,  Tai-Rong Guo ,  Jo-Shu Chang ,  I-Ju Chou

IPC: C02F5/08 ,  C02F1/00 ,  C12N1/02 ,  C12N1/20 ,  C23F11/14 ,  C09K8/528 ,  C07K14/195 ,  C12R1/01

CPC classification number: C12R1/01 ,  C02F5/08 ,  C02F2103/10 ,  C07K14/195 ,  C09K8/528 ,  C12N1/02 ,  C12N1/20 ,  C23F11/14

Abstract: A novel thermophilic bacterium is provided, which has a 16S rDNA sequence as set forth in SEQ ID NO. 1. The thermophilic bacterium excretes extracellular proteins having excellent metal-ion binding ability, being useful in the treatment of boiler equipment, pipelines, geothermal wells or industrial wastewater or hard water.

Abstract translation: 提供了一种新型嗜热细菌，其具有如SEQ ID NO：1所示的16S rDNA序列。 嗜热细菌排泄具有优异金属离子结合能力的细胞外蛋白质，可用于锅炉设备，管道，地热井或工业废水或硬水的处理。