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公开(公告)号：US09034648B2
公开(公告)日：2015-05-19
申请号：US13924181
申请日：2013-06-21
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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公开(公告)号：US08500822B2
公开(公告)日：2013-08-06
申请号：US11628054
申请日：2005-05-31
IPC分类号： A61F2/02
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
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公开(公告)号：US20070259328A1
公开(公告)日：2007-11-08
申请号：US11628054
申请日：2005-05-31
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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公开(公告)号：US20070190645A1
公开(公告)日：2007-08-16
申请号：US10592167
申请日：2005-03-10
CPC分类号： C12N5/069 , A61L27/3804 , A61L27/3895 , A61L27/507 , C12N2501/115 , C12N2533/90 , C12N2535/10
摘要： A vascular cell culture patterning substrate, which can efficiently form a plurality of blood vessels on one substrate. The vascular cell culture patterning substrate includes: a base material; a vascular cell adhesion portion formed in at least two substantially parallel lines on the base material, and having adhesive properties to a vascular cell which forms a blood vessel; and a vascular cell adhesion-inhibiting portion formed in between two adjacent vascular cell adhesion portions on the base material, and inhibiting adhesion to the vascular cell. The vascular cell adhesion-inhibiting portion contains a vascular cell adhesion-inhibiting material having vascular cell adhesion-inhibiting properties of inhibiting adhesion to the vascular cell.
摘要翻译： 一种血管细胞培养图案化衬底，其能够在一个衬底上有效地形成多个血管。 血管细胞培养图案形成基板包括：基材; 形成在所述基材上的至少两条基本上平行的线上的血管细胞粘附部分，并且对形成血管的血管细胞具有粘附性; 以及在基材上的两个相邻的血管细胞粘附部分之间形成的血管细胞粘附抑制部分，并且抑制对血管细胞的粘附。 血管细胞粘附抑制部分含有具有抑制对血管细胞粘附的血管细胞粘附抑制性质的血管细胞粘附抑制物质。
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5.发明申请公开(公告)号：US20070122901A1
公开(公告)日：2007-05-31
申请号：US10574687
申请日：2004-10-15
申请人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
发明人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
IPC分类号： C12N5/06
CPC分类号： C12N5/0068 , A61L27/38 , C12N2533/00
摘要： The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.
摘要翻译： 本发明涉及一种培养细胞的方法，包括以下步骤：使细胞粘附到具有细胞粘附性变化图案的细胞阵列基板的表面，所述细胞粘附变化图案包含具有良好细胞粘附性的区域和图案化的细胞粘附性抑制区域 底物; 以这样的图案化状态将粘附的细胞转移到细胞培养基质; 并培养转移的细胞。
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6.发明申请公开(公告)号：US20070218554A1
公开(公告)日：2007-09-20
申请号：US11587363
申请日：2005-04-25
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C12M23/20 , C12M25/06 , C12M33/00 , C12N2535/10 , C12N2539/10
摘要： A main object of the present invention is to provide a cell culture patterning substrate and a method for manufacturing the same, which can be used to culture the cells on the substrate in an intended shape. To achieve the object, the present invention provides a cell culture patterning substrate comprising: a base material provided with a convex portion; and a cell culture region, which is a region for culturing a cell, formed on a surface of the base material, wherein the cell culture region is partitioned with the convex portion of the base material provided with the convex portion.
摘要翻译： 本发明的主要目的在于提供一种细胞培养图案形成基板及其制造方法，其可以用于将基板上的细胞以期望的形状培养。 为了达到上述目的，本发明提供一种细胞培养图案形成基板，其特征在于，具备：设置有凸部的基材; 和形成在基材表面上的细胞培养区域的细胞培养区域，其中细胞培养区域与设置有凸部的基材的凸部分隔开。
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公开(公告)号：US20050266319A1
公开(公告)日：2005-12-01
申请号：US11044700
申请日：2005-01-27
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2535/10
摘要： The present invention intends primarily to provide such as cells culture patterning substrate that is used to adhere a cell in a highly precise pattern on a base material to culture and a cell culture substrate on which cells are adhered in a high precision pattern. To attain the object, the invention provides a patterning substrate comprising: a base material; and a cell adhesive layer formed on the base material and comprising a cell adhesive material which has cell adhesive properties and is decomposed or denatured by action of a photocatalyst on the basis of irradiation with energy, and a binder.
摘要翻译： 本发明主要提供用于将细胞以高精度图案附着在基材上进行培养的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现该目的，本发明提供了一种图案化衬底，包括：基材; 以及形成在基材上的电池粘合剂层，其包含具有电池粘合性的电池粘合剂材料，并且通过基于能量照射的光催化剂的作用而分解或变性。
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公开(公告)号：US20080213857A1
公开(公告)日：2008-09-04
申请号：US12002863
申请日：2007-12-19
IPC分类号： C12N11/14
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/30 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern.In order to attain the object, the invention provides a patterning substrate comprising a base material, a photocatalyst-containing layer which is formed on the base material and comprises at least a photocatalyst, and a cell adhesion-inhibiting layer which is formed on the photocatalyst-containing layer and comprises a cell adhesion-inhibiting material that has cell adhesion-inhibiting properties of inhibiting adhesion to cells and is decomposed or denatured by action of the photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基材和以高精度图案粘附细胞的细胞培养基质。 为了达到上述目的，本发明提供一种图案形成基材，其包含基材，含有光催化剂的层，其形成在基材上，并且至少包含光催化剂，以及在光催化剂上形成的细胞粘附抑制层 并且包含细胞粘附抑制材料，其具有抑制细胞粘附的细胞粘附抑制性质，并且通过基于光能照射的光催化剂的作用而分解或变性。
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公开(公告)号：US20060183219A1
公开(公告)日：2006-08-17
申请号：US11044124
申请日：2005-01-27
CPC分类号： C12Q1/045 , C12N5/0068 , C12N2533/30 , C12N2533/40
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells are adhered in a highly precise pattern. To attain the object, the invention provides a patterning substrate comprising: a base material; and a cell adhesion-inhibiting layer which is formed on the base material and comprises a cell adhesion-inhibiting material that has cell adhesion-inhibiting properties of inhibiting adhesion to cells and is denatured by action of a photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现该目的，本发明提供了一种图案化衬底，包括：基材; 以及细胞粘附抑制层，其形成在基材上，并且包含细胞粘附抑制材料，其具有抑制细胞粘附的细胞粘附抑制性质，并且通过基于光能照射的光催化剂的作用而变性。
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公开(公告)号：US20060019390A1
公开(公告)日：2006-01-26
申请号：US11044978
申请日：2005-01-27
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2533/52 , C12N2533/56 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern. To attain the above-mentioned object, the invention provides a patterning substrate, comprising a base material, a photocatalyst-containing layer which is formed on the base material and comprises at least a photocatalyst, and a cell adhesive layer which is formed on the photocatalyst-containing layer and at least comprises a cell adhesive material that has cell adhesive properties and is decomposed or denatured by action of the photocatalyst on the basis of irradiation with energy.
摘要翻译： 本发明主要提供用于将细胞以高精度图案粘附到培养基材上的细胞培养图案形成基材和以高精度图案粘附细胞的细胞培养基质。 为了实现上述目的，本发明提供了一种图案化衬底，其包括基材，在基材上形成的至少包含光催化剂的光催化剂层和形成在光催化剂上的电池粘合剂层 并且至少包含具有细胞粘合性质的细胞粘合剂材料，并且在光能照射的基础上通过光催化剂的作用而分解或变性。
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