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1.发明申请公开(公告)号：US20070122901A1
公开(公告)日：2007-05-31
申请号：US10574687
申请日：2004-10-15
申请人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
发明人： Ikuo Morita , Makoto Nakamura , Hideyuki Miyake , Hideshi Hattori , Hironori Kobayashi , Masaaki Kurihara
IPC分类号： C12N5/06
CPC分类号： C12N5/0068 , A61L27/38 , C12N2533/00
摘要： The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.
摘要翻译： 本发明涉及一种培养细胞的方法，包括以下步骤：使细胞粘附到具有细胞粘附性变化图案的细胞阵列基板的表面，所述细胞粘附变化图案包含具有良好细胞粘附性的区域和图案化的细胞粘附性抑制区域 底物; 以这样的图案化状态将粘附的细胞转移到细胞培养基质; 并培养转移的细胞。
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公开(公告)号：US08500822B2
公开(公告)日：2013-08-06
申请号：US11628054
申请日：2005-05-31
IPC分类号： A61F2/02
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
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公开(公告)号：US20070259328A1
公开(公告)日：2007-11-08
申请号：US11628054
申请日：2005-05-31
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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公开(公告)号：US09034648B2
公开(公告)日：2015-05-19
申请号：US13924181
申请日：2013-06-21
CPC分类号： C12N5/0691 , A61L27/36 , A61L27/3886 , A61L2430/36 , C12N5/0697
摘要： An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
摘要翻译： 本发明的目的是提供一种人造组织结构，其具有用于运输营养物，氧气，废物等的手段，并且在体内是可行的。 本发明涉及体外形成的组织构建体，其包括血管层，基底膜层和组织形成细胞层。
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5.发明授权Method for producing cell culture substrate and apparatus for producing cell culture substrate 失效细胞培养基质的制造方法和细胞培养基材的制造装置公开(公告)号：US07687251B2
公开(公告)日：2010-03-30
申请号：US10594174
申请日：2005-03-24
CPC分类号： C12M25/06 , C12M33/00 , C12N5/0068 , C12N2535/10 , Y10S977/703
摘要： A method for producing a cell culture substrate to which cells adhere in a finely processed pattern while retaining the pattern for a long period of time to culture the cells and a production apparatus used in the production method. The method includes: forming a patterning substrate by forming: on a base material, a light shielding portion, and a cell adhesive layer having adhesion to a cell and containing a cell adhesive material which is decomposed or denatured by an action of a photocatalyst upon energy irradiation so as to cover the light shielding portion; an energy irradiating process of irradiating energy to the patterning substrate from the base material side to form a pattern; and a cell adhesion process of making the cell adhere to the cell adhesion portion in a cell culture medium containing the cell and a culture medium.
摘要翻译： 细胞培养基板的制造方法，其中细胞以精细加工的图案粘附，同时长期保持图案以培养细胞，以及生产方法中使用的制造装置。 该方法包括：通过在基底材料，遮光部分和具有对细胞粘附的细胞粘合剂层上形成图案形成衬底，并且含有通过光催化剂的作用而被分解或变性的细胞粘附材料的能量 照射以覆盖遮光部分; 能量照射处理，其从所述基材侧向所述图案化衬底照射能量以形成图案; 以及在含有细胞和培养基的细胞培养基中使细胞粘附于细胞粘附部的细胞粘附过程。
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公开(公告)号：US20050186674A1
公开(公告)日：2005-08-25
申请号：US10986641
申请日：2004-11-12
CPC分类号： C12N5/0068 , C12M25/00 , C12N2535/10
摘要： The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere a cell in a highly precise pattern on a base material to culture and a cell culture substrate on which a cell is adhered in a high precision pattern. In order to achieve the above-mentioned object, the present invention provides a patterning substrate having a base material and a cell culture patterning layer that is formed on the base material and has at least a photocatalyst and a cell adhesive material that has the adhesiveness with the cell and is decomposed or modified owing to an action of the photocatalyst in combination with energy irradiation.
摘要翻译： 本发明主要提供用于将细胞以高精度图案附着在基材上培养的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现上述目的，本发明提供一种图案形成基板，其具有基材和细胞培养图案形成层，所述基材和细胞培养图案化层形成在所述基材上，并且至少具有光催化剂和具有与 由于光催化剂的作用与能量照射的结合而被分解或改性。
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7.发明申请公开(公告)号：US20070218554A1
公开(公告)日：2007-09-20
申请号：US11587363
申请日：2005-04-25
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C12M23/20 , C12M25/06 , C12M33/00 , C12N2535/10 , C12N2539/10
摘要： A main object of the present invention is to provide a cell culture patterning substrate and a method for manufacturing the same, which can be used to culture the cells on the substrate in an intended shape. To achieve the object, the present invention provides a cell culture patterning substrate comprising: a base material provided with a convex portion; and a cell culture region, which is a region for culturing a cell, formed on a surface of the base material, wherein the cell culture region is partitioned with the convex portion of the base material provided with the convex portion.
摘要翻译： 本发明的主要目的在于提供一种细胞培养图案形成基板及其制造方法，其可以用于将基板上的细胞以期望的形状培养。 为了达到上述目的，本发明提供一种细胞培养图案形成基板，其特征在于，具备：设置有凸部的基材; 和形成在基材表面上的细胞培养区域的细胞培养区域，其中细胞培养区域与设置有凸部的基材的凸部分隔开。
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公开(公告)号：US20070190645A1
公开(公告)日：2007-08-16
申请号：US10592167
申请日：2005-03-10
CPC分类号： C12N5/069 , A61L27/3804 , A61L27/3895 , A61L27/507 , C12N2501/115 , C12N2533/90 , C12N2535/10
摘要： A vascular cell culture patterning substrate, which can efficiently form a plurality of blood vessels on one substrate. The vascular cell culture patterning substrate includes: a base material; a vascular cell adhesion portion formed in at least two substantially parallel lines on the base material, and having adhesive properties to a vascular cell which forms a blood vessel; and a vascular cell adhesion-inhibiting portion formed in between two adjacent vascular cell adhesion portions on the base material, and inhibiting adhesion to the vascular cell. The vascular cell adhesion-inhibiting portion contains a vascular cell adhesion-inhibiting material having vascular cell adhesion-inhibiting properties of inhibiting adhesion to the vascular cell.
摘要翻译： 一种血管细胞培养图案化衬底，其能够在一个衬底上有效地形成多个血管。 血管细胞培养图案形成基板包括：基材; 形成在所述基材上的至少两条基本上平行的线上的血管细胞粘附部分，并且对形成血管的血管细胞具有粘附性; 以及在基材上的两个相邻的血管细胞粘附部分之间形成的血管细胞粘附抑制部分，并且抑制对血管细胞的粘附。 血管细胞粘附抑制部分含有具有抑制对血管细胞粘附的血管细胞粘附抑制性质的血管细胞粘附抑制物质。
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公开(公告)号：US20050266319A1
公开(公告)日：2005-12-01
申请号：US11044700
申请日：2005-01-27
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2535/10
摘要： The present invention intends primarily to provide such as cells culture patterning substrate that is used to adhere a cell in a highly precise pattern on a base material to culture and a cell culture substrate on which cells are adhered in a high precision pattern. To attain the object, the invention provides a patterning substrate comprising: a base material; and a cell adhesive layer formed on the base material and comprising a cell adhesive material which has cell adhesive properties and is decomposed or denatured by action of a photocatalyst on the basis of irradiation with energy, and a binder.
摘要翻译： 本发明主要提供用于将细胞以高精度图案附着在基材上进行培养的细胞培养图案形成基板和以高精度图案粘附细胞的细胞培养基板。 为了实现该目的，本发明提供了一种图案化衬底，包括：基材; 以及形成在基材上的电池粘合剂层，其包含具有电池粘合性的电池粘合剂材料，并且通过基于能量照射的光催化剂的作用而分解或变性。
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公开(公告)号：US20070148762A1
公开(公告)日：2007-06-28
申请号：US10589376
申请日：2005-02-18
IPC分类号： C12M3/00
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2535/10
摘要： A cell culture patterning substrate on which cells can be arranged regularly with high efficiency over a large area on a base material so as to attain formation of a tissue, etc. The cell culture patterning substrate includes: a base material; and a cell culture region which is formed on the base material, is a region for culturing a cell and contains a cell adhesive layer having adhesive properties to the cell. The cell culture region includes: a cell adhesion portion at which the cell adhesive layer is formed; and a cell adhesion auxiliary portion, formed in a pattern, which inhibits adhesion to the cell, and the cell adhesion auxiliary portion is formed such that, upon adhesion of the cell to the cell adhesion portion, the cells on two cell adhesion portions adjacent to the cell adhesion auxiliary portion can be bound to each other on the cell adhesion auxiliary portion.
摘要翻译： 一种细胞培养图案形成基板，其上可以在基材上大面积地高效率地排列细胞以形成组织等。细胞培养图案形成基板包括：基材; 并且在基材上形成的细胞培养区是用于培养细胞的区域，并且含有对细胞具有粘合性的细胞粘合剂层。 细胞培养区域包括：形成细胞粘合剂层的细胞粘附部分; 和形成为抑制与细胞粘合的图案的细胞粘附辅助部分和细胞粘附辅助部分形成，使得当细胞粘附到细胞粘附部分时，细胞粘附在两个细胞粘附部分上 细胞粘附辅助部分可以在细胞粘附辅助部分上彼此结合。
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