公开(公告)号：US11891609B2

公开(公告)日：2024-02-06

申请号：US17680289

申请日：2022-02-25

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

CPC classification number: C12N15/74 ,  C12N1/20 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

公开(公告)号：US20220177899A1

公开(公告)日：2022-06-09

申请号：US17680289

申请日：2022-02-25

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US11965154B2

公开(公告)日：2024-04-23

申请号：US16545097

申请日：2019-08-20

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Isaac Wagner ,  Clint Davis ,  Julia Swavola ,  Phillip Belgrader

IPC: C12N1/20 ,  B01L3/00 ,  C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12M3/04 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: C12N1/20 ,  B01L3/502 ,  C12M27/10 ,  C12M29/04 ,  C12M35/00 ,  C12M35/02 ,  C12M45/00 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00722 ,  G01N35/10 ,  B01L2200/16 ,  B01L2300/087 ,  C12M23/44 ,  G01N2035/009

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：US11319542B2

公开(公告)日：2022-05-03

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

公开(公告)号：US20220081693A1

公开(公告)日：2022-03-17

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US20200071660A1

公开(公告)日：2020-03-05

申请号：US16545097

申请日：2019-08-20

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Isaac Wagner ,  Clint Davis ,  Julia Swavola ,  Phillip Belgrader

IPC: C12N1/20 ,  C12M1/00 ,  G01N35/10 ,  G01N35/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/04 ,  C12N13/00

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：US20220025359A1

公开(公告)日：2022-01-27

申请号：US17300518

申请日：2021-07-27

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Clint Davis ,  Charles Johnson ,  Andrew Garst

IPC: C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N9/22

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

公开(公告)号：US11203762B2

公开(公告)日：2021-12-21

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US20210147858A1

公开(公告)日：2021-05-20

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N9/22 ,  C12N1/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US20200056192A1

公开(公告)日：2020-02-20

申请号：US16540767

申请日：2019-08-14

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Charles Johnson ,  Clint Davis

IPC: C12N15/70 ,  C12M3/00 ,  C12M1/42 ,  C12M1/12 ,  C12N15/90

Abstract: The present disclosure provides methods, automated modules, and instruments for enrichment of live cells that have been edited by nucleic acid-guided nuclease genome editing. The disclosure provides improved methods and modules—including high throughput methods and modules—for enriching for cells that have been subjected to editing.