公开(公告)号：US20200056214A1

公开(公告)日：2020-02-20

申请号：US16670042

申请日：2019-10-31

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10 ,  C08G63/06

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US20170009221A1

公开(公告)日：2017-01-12

申请号：US15115394

申请日：2015-01-30

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12N9/88 ,  C12P7/62 ,  C12N15/74

CPC classification number: C12N9/88 ,  C12N15/74 ,  C12P7/625 ,  C12Y402/01017 ,  C12Y402/01074

Abstract: This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.

Abstract translation: 本发明涉及产生具有调节的单体组成比的聚羟基链烷酸酯（PHA）共聚物的微生物，并且在基因组DNA中包含（R） - 特异性烯酰辅酶A水合酶基因，其中（R） - 特异性 烯酰辅酶A水合酶基因包含由一个或多个核苷酸的取代，缺失，插入和/或添加组成的修饰物，使得（ R） - 特异性烯酰辅酶A水合酶基因，以及使用该微生物生产PHA共聚物的方法。

公开(公告)号：US20160237462A1

公开(公告)日：2016-08-18

申请号：US14437672

申请日：2013-10-22

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N15/74

CPC classification number: C12P7/625 ,  C12N9/18 ,  C12N15/74

Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.

Abstract translation: 本发明的目的是提供一种积累高分子量PHA的微生物菌株和使用该微生物的PHA生产方法。 本发明提供一种生产PHA共聚物的方法，其包括培养微生物，其中微生物的以下基因（a）和（b）中的至少一部分已经被替换，缺失，插入和 /或添加以减少或消除由该基因编码的PHA降解酶的活性：（a）编码序列表中SEQ ID NO：2的氨基酸序列的PHA降解酶基因; 和（b）编码与序列表中SEQ ID NO：2的氨基酸序列具有至少85％序列同一性且具有PHA降解酶活性的多肽的基因。

公开(公告)号：US20170198313A1

公开(公告)日：2017-07-13

申请号：US15129726

申请日：2015-03-27

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10

CPC classification number: C12P7/625 ,  C08G63/06 ,  C08L101/16 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/09 ,  C12Y203/01 ,  C12Y402/01017

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.