公开(公告)号：US11421237B2

公开(公告)日：2022-08-23

申请号：US16857089

申请日：2020-04-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sung Su Lim ,  Seul Gi Shin ,  Yun Kyung Kim

IPC: C12N15/64 ,  C07K14/47

Abstract: The present disclosure relates to a vector pair for screening TDP-43 (TAR DNA-binding protein 43 or TransActive Response DNA-binding protein 43) oligomer formation, a cell line transfected with the vector pair, and a method of monitoring TDP-43 oligomer formation using the cell line. More specifically, the vector pair includes: a first vector including a first TDP-43 gene and a first fluorescence protein gene; and a second vector including a second TDP-43 gene and a second fluorescence protein gene, wherein a protein expressed from the first fluorescence protein gene and a protein expressed from the second fluorescence protein gene bind to each other to display fluorescence, by association between a protein expressed from the first TDP-43 gene and a protein expressed from the second TDP-43 gene. The vector pair is effective in that it makes it possible to monitor TDP-43 oligomer formation in living cells.

公开(公告)号：US09962384B1

公开(公告)日：2018-05-08

申请号：US15697728

申请日：2017-09-07

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Yun Kyung Kim ,  Sung Su Lim ,  Do Hee Kim ,  Md. Mamunul Haque ,  Seul Gi Shin ,  Ha Eun Lee ,  Tae Hun Kim ,  Sang Min Lim ,  Ae Nim Pae

IPC: A61K31/50 ,  A61K47/36 ,  C07D237/10

CPC classification number: A61K31/50 ,  A61K47/36 ,  C07D237/04 ,  C07D237/10

Abstract: Disclosed is a levosimendan compound, an optically active isomer thereof, or a pharmaceutically acceptable salt thereof, which is used for the prevention and/or treatment of diseases caused by tau aggregation or phosphorylation, such as neurodegenerative diseases (e.g., Alzheimer disease). In accordance with yet another aspect of the present invention, there is provided a health functional food for preventing or treating tau aggregation-related diseases, containing a levosimendan compound or a pharmaceutically acceptable salt, stereoisomer or solvate thereof.

公开(公告)号：US10544427B2

公开(公告)日：2020-01-28

申请号：US15709822

申请日：2017-09-20

Applicant: Korea Institute of Science and Technology

Inventor： Do Hee Kim ,  Seul Gi Shin ,  Sung Su Lim ,  Ae Nim Pae ,  Dong Jin Kim ,  Yun Kyung Kim

IPC: A01K67/027 ,  C12N15/85 ,  C07K14/47 ,  G01N33/50 ,  G01N33/68

Abstract: Disclosed is a vector pair for screening tau oligomer formation, a mouse embryo introduced with the vector pair, a transgenic model mouse of neurological disease, obtained from the mouse embryo, and a method of screening a tau oligomer formation inhibitor candidate using the transgenic model mouse. More specifically, the present invention provides vector pair for screening tau oligomer formation, comprising: a first vector comprising a first tau gene, a first fluorescence protein gene and a first neuron-specific promoter; and a second vector comprising a second tau gene, a second fluorescence protein gene and a second neuron-specific promoter, wherein a protein expressed from the first fluorescence protein gene and a protein expressed from the second fluorescence protein gene bind to each other to display fluorescence, by association between a protein expressed from the first tau gene and a protein expressed from the second tau gene.

公开(公告)号：US20180119169A1

公开(公告)日：2018-05-03

申请号：US15709822

申请日：2017-09-20

Applicant: Korea Institute of Science and Technology

Inventor： Do Hee KIM ,  Seul Gi Shin ,  Sung Su Lim ,  Ae Nim Pae ,  Dong Jin Kim ,  Yun Kyung Kim

IPC: C12N15/85 ,  A01K67/027 ,  G01N33/50 ,  C07K14/47 ,  G01N33/68

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/05 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/0312 ,  A01K2267/0318 ,  C07K14/47 ,  C07K2319/60 ,  C12N2015/8527 ,  C12N2015/8536 ,  C12N2015/859 ,  C12N2830/008 ,  G01N33/5058 ,  G01N33/5088 ,  G01N33/6896 ,  G01N2800/2814

Abstract: Disclosed is a vector pair for screening tau oligomer formation, a mouse embryo introduced with the vector pair, a transgenic model mouse of neurological disease, obtained from the mouse embryo, and a method of screening a tau oligomer formation inhibitor candidate using the transgenic model mouse. More specifically, the present invention provides vector pair for screening tau oligomer formation, comprising: a first vector comprising a first tau gene, a first fluorescence protein gene and a first neuron-specific promoter; and a second vector comprising a second tau gene, a second fluorescence protein gene and a second neuron-specific promoter, wherein a protein expressed from the first fluorescence protein gene and a protein expressed from the second fluorescence protein gene bind to each other to display fluorescence, by association between a protein expressed from the first tau gene and a protein expressed from the second tau gene.