公开(公告)号：US20130210674A1

公开(公告)日：2013-08-15

申请号：US13743217

申请日：2013-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ian A. HARDING ,  Donald SANDELL ,  Kevin BODNER ,  Jaynish PATEL ,  Timothy WOUDENBERG ,  Mark OLDHAM ,  H. Pin KAO ,  Gary LIM ,  John P. BODEAU ,  Dar BAHATT ,  Sergey V. ERMAKOV ,  Adrian FAWCETT ,  Jones C. Jones

IPC: C12N15/10

CPC classification number: C12N15/1072 ,  B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2300/1805

Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.

公开(公告)号：US20140170656A1

公开(公告)日：2014-06-19

申请号：US14137376

申请日：2013-12-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Muhammad A. SHARAF ,  Timothy WOUDENBERG ,  Khairuzzaman Bashar MULLAH

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6876 ,  G01N27/44726 ,  G01N27/44791 ,  C12Q2565/631

Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.

Abstract translation: 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。