公开(公告)号：US20180371008A1

公开(公告)日：2018-12-27

申请号：US16023051

申请日：2018-06-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun LEE ,  Weidong ZHENG

IPC: C07H21/04 ,  C07H21/02 ,  C12Q1/6813 ,  C07H1/00

Abstract: The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.

公开(公告)号：US20150126723A1

公开(公告)日：2015-05-07

申请号：US14533029

申请日：2014-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun LEE ,  Weidong ZHENG

IPC: C07H21/04 ,  C07H1/00

CPC classification number: C07H21/04 ,  C07H1/00 ,  C07H21/02 ,  C12Q1/6813

Abstract: The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.

Abstract translation: 本发明通常涉及用于合成核酸分子，特别是用于合成标记的核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及用于合成间接标记的核酸分子的方法，试剂盒和组合物。 根据本发明生产的标记的核酸分子特别适用于用于核酸检测，诊断和阵列分析的标记探针。

公开(公告)号：US20160272955A1

公开(公告)日：2016-09-22

申请号：US15173439

申请日：2016-06-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Weidong ZHENG ,  Jun Lee ,  Robert Potter ,  David Mandelman

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12N9/1276 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y207/07007 ,  Y02P20/52 ,  C12Q2561/101 ,  C12Q2527/149 ,  C12Q2521/101

Abstract: A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.

Abstract translation: 一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。 该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法。