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    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    3.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 有权

    公开(公告)号：US20220315917A1

    公开(公告)日：2022-10-06

    申请号：US17302674

    申请日：2021-05-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Daniel MAZUR ,  Sihong CHEN ,  Guobin LUO ,  Xinzhan PENG

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    POLYMERASE COMPOSITIONS AND METHODS
    5.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS 有权
    聚合物组合物和方法

    公开(公告)号：US20150247136A1

    公开(公告)日：2015-09-03

    申请号：US14710160

    申请日：2015-05-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Stephen P. HENDRICKS ,  Michael PHELAN ,  Marian PERIS ,  Cheng-Yao CHEN ,  Daniel MAZUR ,  Xinzhan PENG ,  Amy CASTILLO

    IPC: C12N9/12

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

    Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。

    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION
    6.
    发明申请
    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 有权
    使用能量转移检测的单分子序列的方法和装置

    公开(公告)号：US20150118679A1

    公开(公告)日：2015-04-30

    申请号：US14584829

    申请日：2014-12-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

    Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

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