公开(公告)号：US20150118679A1

公开(公告)日：2015-04-30

申请号：US14584829

申请日：2014-12-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

公开(公告)号：US20140234853A1

公开(公告)日：2014-08-21

申请号：US14108166

申请日：2013-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Cheng-Yao CHEN ,  Guobin LUO ,  Michael PREVITE ,  Jamshid TEMIROV ,  Theo NIKIFOROV ,  Zhaohui ZHOU ,  Hongye SUN ,  Yufang WANG ,  Stefanie Yukiko NISHIMURA ,  Hongyi WANG ,  Marian PERIS ,  Barnett ROSENBLUM ,  Michael PHELAN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract translation: 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。 该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

公开(公告)号：US20230193223A1

公开(公告)日：2023-06-22

申请号：US17932844

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter VANDER HORN ,  Cheng-Yao CHEN ,  Guobin LUO ,  Michael PREVITE ,  Jamshid TEMIROV ,  Theo NIKIFOROV ,  Zhaohui ZHOU ,  Hongye SUN ,  Yufang WANG ,  Stefanie Yukiko NISHIMURA ,  Hongyi WANG ,  Marian PERIS ,  Barnett ROSENBLUM ,  Michael PHELAN

IPC: C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US20200231948A1

公开(公告)日：2020-07-23

申请号：US16825788

申请日：2020-03-20

Applicant: Life Technologies Corporation

Inventor： Peter VANDER HORN ,  Cheng-Yao CHEN ,  Guobin LUO ,  Michael PREVITE ,  Jamshid TEMIROV ,  Theo NIKIFOROV ,  Zhaohui ZHOU ,  Hongye SUN ,  Yufang WANG ,  Stefanie Yukiko NISHIMURA ,  Hongyi WANG ,  Marian PERIS ,  Barnett ROSENBLUM ,  Michael PHELAN

IPC: C12N9/12 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/68

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

公开(公告)号：US20190062829A1

公开(公告)日：2019-02-28

申请号：US16123140

申请日：2018-09-06

Applicant: Life Technologies Corporation

Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

IPC: C12Q1/6869 ,  C12N9/12 ,  G01N33/58 ,  G01N21/64 ,  C07H19/20 ,  C12Q1/6818 ,  C12N9/96

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

公开(公告)号：US20170283868A1

公开(公告)日：2017-10-05

申请号：US15621341

申请日：2017-06-13

Applicant: Life Technologies Corporation

Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

IPC: C12Q1/68 ,  G01N21/64 ,  C12N9/96 ,  C12N9/12 ,  C07H19/20 ,  G01N33/58

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

公开(公告)号：US20170369857A1

公开(公告)日：2017-12-28

申请号：US15690945

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Peter VANDER HORN ,  Cheng-Yao CHEN ,  Guobin LUO ,  Michael PREVITE ,  Jamshid TEMIROV ,  Theo NIKIFOROV ,  Zhaohui ZHOU ,  Hongye SUN ,  Yufang WANG ,  Stefanie Yukiko NISHIMURA ,  Hongyi WANG ,  Marian PERIS ,  Barnett ROSENBLUM ,  Michael PHELAN

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.