公开(公告)号：US20180374920A1

公开(公告)日：2018-12-27

申请号：US16107953

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric WELCH ,  Joseph BARTEL ,  Eric TULSKY ,  Joseph TREADWAY ,  Yongfen CHEN

IPC: H01L29/06 ,  H01L51/00 ,  H01L33/04 ,  H01L31/0352 ,  H01L21/02 ,  G01N33/58 ,  G01N33/533 ,  C30B29/60 ,  B82Y30/00 ,  C30B29/48 ,  C30B29/40 ,  C30B7/00 ,  B82Y40/00 ,  B82Y20/00

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

公开(公告)号：US20140308730A1

公开(公告)日：2014-10-16

申请号：US14087307

申请日：2013-11-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo NIKIFOROV ,  Daniel MAZUR ,  Xinzhan PENG ,  Tommie Lloyd LINCECUM ,  Yuri BELOSLUDTSEV ,  Howard REESE ,  Dmitriy GREMYACHINSKIY ,  Roman ROZHKOV ,  John MAURO ,  Joseph BEECHEM ,  Eric TULSKY ,  Imad NAASANI ,  Kari HALEY ,  Joseph TREADWAY

IPC: C12N9/12

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract translation: 本文公开了包含连接到具有生物活性并可用于多种生物应用的标签的生物分子的缀合物。 例如，本文提供了聚合酶 - 纳米颗粒缀合物，其包括与纳米颗粒连接的聚合酶，其中缀合物具有聚合酶活性。 这样的缀合物可以表现出减少的聚集和改进的沉淀物，其中平均生物分子：纳米颗粒比接近或等于1:1。 本文还公开了制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，例如核苷酸掺入，引物延伸和单分子测序。

公开(公告)号：US20190062830A1

公开(公告)日：2019-02-28

申请号：US16123166

申请日：2018-09-06

Applicant: Life Technologies Corporation

Inventor： Theo NIKIFOROV ,  Daniel MAZUR ,  Xinzhan PENG ,  Tommie Lloyd LINCECUM ,  Yuri BELOSLUDTSEV ,  Howard REESE ,  Dmitriy GREMYACHINSKIY ,  Roman ROZHKOV ,  John MAURO ,  Joseph BEECHEM ,  Eric TULSKY ,  Imad NAASANI ,  Kari HALEY ,  Joseph TREADWAY

IPC: C12Q1/6869 ,  C12N9/12 ,  G01N33/58 ,  G01N21/64 ,  C07H19/20 ,  C12Q1/6818 ,  C12N9/96

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US20160265044A1

公开(公告)日：2016-09-15

申请号：US15166372

申请日：2016-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo NIKIFOROV ,  Daniel MAZUR ,  Xinzhan PENG ,  Tommie Lloyd LINCECUM ,  Yuri BELOSLUDTSEV ,  Howard REESE ,  Dmitriy GREMYACHINSKIY ,  Roman ROZHKOV ,  John MAURO ,  Joseph BEECHEM ,  Eric TULSKY ,  Imad NAASANI ,  Kari HALEY ,  Joseph TREADWAY

IPC: C12Q1/68 ,  C12N9/12 ,  G01N21/64 ,  C12N9/96

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract translation: 本文公开了包含连接到具有生物活性并可用于多种生物应用的标签的生物分子的缀合物。 例如，本文提供了聚合酶 - 纳米颗粒缀合物，其包括与纳米颗粒连接的聚合酶，其中缀合物具有聚合酶活性。 这样的缀合物可以表现出减少的聚集和改进的沉淀物，其中平均生物分子：纳米颗粒比接近或等于1:1。 本文还公开了制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，例如核苷酸掺入，引物延伸和单分子测序。

公开(公告)号：US20150233936A1

公开(公告)日：2015-08-20

申请号：US14590840

申请日：2015-01-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric TULSKY ,  Kari Haley ,  Imad Naasani ,  John Mauro ,  Roman Rozhkov ,  Joseph Treadway

IPC: G01N33/58 ,  G01N21/64 ,  G01N1/30

CPC classification number: G01N33/588 ,  B01J13/14 ,  B22F1/0022 ,  B22F1/02 ,  B82Y30/00 ,  G01N1/30 ,  G01N21/64 ,  G01N2001/302 ,  Y10S977/773 ,  Y10S977/81 ,  Y10S977/834 ,  Y10S977/902

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Abstract translation: 本文提供了制备包含核/壳纳米晶体的水溶性纳米颗粒的方法，其被包覆有包含足够亲水性配体的表面层以使纳米颗粒水溶性或水分散。 还提供了在纳米颗粒表面上交联分子的方法，该方法可用于上述水溶性纳米颗粒。 还提供了由这些方法得到的纳米颗粒组合物。