公开(公告)号：US20250019864A1

公开(公告)日：2025-01-16

申请号：US18606205

申请日：2024-03-15

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C07K14/725 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20240428885A1

公开(公告)日：2024-12-26

申请号：US18748533

申请日：2024-06-20

Applicant: Life Technologies Corporation

Inventor： Fiona Hyland ,  Asha Kamat ,  Timothy Looney

IPC: G16B20/20 ,  G06F16/2457 ,  G06F16/248 ,  G06N3/126

Abstract: The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.

公开(公告)号：US10920273B2

公开(公告)日：2021-02-16

申请号：US15873862

申请日：2018-01-17

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US12227878B2

公开(公告)日：2025-02-18

申请号：US17302638

申请日：2021-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Geoffrey Lowman ,  Timothy Looney ,  Lifeng Lin ,  Elizabeth Linch ,  Lauren Miller

IPC: C40B40/08 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6869 ,  C40B50/00 ,  C40B50/06

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US11970787B2

公开(公告)日：2024-04-30

申请号：US17248722

申请日：2021-02-04

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

CPC classification number: C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/7051 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US11610648B2

公开(公告)日：2023-03-21

申请号：US16851472

申请日：2020-04-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Fiona Hyland ,  Asha Kamat ,  Timothy Looney

IPC: G16B20/20 ,  G06F16/2457 ,  G06F16/248 ,  G06N3/126

Abstract: The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.

公开(公告)号：US20230416810A1

公开(公告)日：2023-12-28

申请号：US18317484

申请日：2023-05-15

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Chenchen Yang ,  Timothy Looney

IPC: C12Q1/6844 ,  C12Q1/6883

CPC classification number: C12Q1/6844 ,  C12Q1/6883 ,  C12Q2600/16

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of B cell receptor heavy and light chains in a single assay, with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20230282306A1

公开(公告)日：2023-09-07

申请号：US18170797

申请日：2023-02-17

Applicant: Life Technologies Corporation

Inventor： Fiona Hyland ,  Asha Kamat ,  Timothy Looney

IPC: G16B20/20 ,  G06F16/2457 ,  G06F16/248 ,  G06N3/126

CPC classification number: G16B20/20 ,  G06F16/24575 ,  G06F16/248 ,  G06N3/126

Abstract: The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.

公开(公告)号：US20240392386A1

公开(公告)日：2024-11-28

申请号：US18762451

申请日：2024-07-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Looney

IPC: C12Q1/6886 ,  C40B30/04

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor and/or B cell receptor repertoires in samples prior to a treatment and predicting a subject's response to the treatment based on the measured convergence frequency. In another aspect, methods provide for an immune receptor haplotype group and predicting a subject's potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.

公开(公告)号：US12065705B2

公开(公告)日：2024-08-20

申请号：US15733628

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Looney

IPC: C40B30/04 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16 ,  C40B30/04

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor and/or B cell receptor repertoires in samples prior to a treatment and predicting a subjects response to the treatment based on the measured convergence frequency. In another aspect, methods provide for an immune receptor haplotype group and predicting a subjects potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.