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    Methods for tissue culture and transformation of sugarcane
    1.
    发明申请
    Methods for tissue culture and transformation of sugarcane 审中-公开
    甘蔗组织培养和转化方法

    公开(公告)号：US20130055472A1

    公开(公告)日：2013-02-28

    申请号：US13599003

    申请日：2012-08-30

    申请人： Myeong-Je Cho ,  Theodore M. Klein ,  Zuo-Yu Zhao

    发明人： Myeong-Je Cho ,  Theodore M. Klein ,  Zuo-Yu Zhao

    IPC分类号： A01H1/06 ,  C12N5/04

    CPC分类号： C12N15/8205 ,  A01H4/00

    摘要： Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.

    摘要翻译： 提供单叶植物有效转化和再生的组合物和方法。 转化方法涉及土壤杆菌感染。 以这种方式，任何感兴趣的基因可以以高转化效率和低拷贝数引入单子叶植物。 还提供了转化和再生的单子叶植物细胞，组织，植物和种子。 本发明包括再生转化的植物，由其产生的转基因种子，以及来自后代的转基因植物和转基因种子。

    AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS
    3.
    发明申请
    AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS 有权
    DICOT植物的农杆菌介导转化

    公开(公告)号：US20120255070A1

    公开(公告)日：2012-10-04

    申请号：US13438077

    申请日：2012-04-03

    申请人： Hyeon-Je Cho ,  Linda A. Castle ,  Theodore M. Klein ,  Tai Minh ,  York Moy

    发明人： Hyeon-Je Cho ,  Linda A. Castle ,  Theodore M. Klein ,  Tai Minh ,  York Moy

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8205 ,  C12N1/20

    摘要： Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.

    摘要翻译： 提供组合物和方法用于双子叶植物的有效转化。 更具体地，本发明的组合物和方法用于农业用于双子叶植物的农杆菌介导的转化。 组合物包括含有高浓度蔗糖和葡萄糖的培养基。 栽培培养基可用于涉及农杆菌介导的具有感兴趣基因的双子叶植物转化的方法。 以这种方式，可以将任何感兴趣的基因导入具有高转化效率和减少的组织坏死的双子叶植物。

    METHOD FOR TRANSFORMING SOMATIC EMBRYOS
    4.
    发明申请
    METHOD FOR TRANSFORMING SOMATIC EMBRYOS 审中-公开
    用于改变人造胚胎的方法

    公开(公告)号：US20110162111A1

    公开(公告)日：2011-06-30

    申请号：US12976542

    申请日：2010-12-22

    申请人： Theodore M. Klein

    发明人： Theodore M. Klein

    IPC分类号： C12N15/87 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8202 ,  C12N15/8206

    摘要： Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.

    摘要翻译： 公开了用目的核酸转化植物细胞并从其再生植物的方法。 该方法对双子叶植物或单子叶植物成熟体细胞胚的转化特别有用。

    Gene Expression Modulating Element
    5.
    发明申请
    Gene Expression Modulating Element 有权

    公开(公告)号：US20100083408A1

    公开(公告)日：2010-04-01

    申请号：US12626962

    申请日：2009-11-30

    申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/10 ,  C07H21/00

    CPC分类号： C12N15/8216

    摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

    Plant transcription factors
    8.
    发明授权
    Plant transcription factors 有权
    植物转录因子

    公开(公告)号：US07888560B1

    公开(公告)日：2011-02-15

    申请号：US11899370

    申请日：2007-09-04

    申请人： Rebecca E. Cahoon ,  Theodore M. Klein

    发明人： Rebecca E. Cahoon ,  Theodore M. Klein

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/29 ,  C07K14/415

    CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

    摘要： This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.

    摘要翻译： 本发明涉及编码转录因子的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分转录因子的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中积累的油的水平的改变。

    Gene expression modulating element
    9.
    发明授权
    Gene expression modulating element 失效
    基因表达调控元件

    公开(公告)号：US07655786B2

    公开(公告)日：2010-02-02

    申请号：US11685347

    申请日：2007-03-13

    申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

    IPC分类号： C07H21/04 ,  C12N15/00

    CPC分类号： C12N15/8216

    摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

    摘要翻译： 本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。 本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法：a）定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b）取该基因 区域或该基因间区域的一部分，并将其加入到包含分离的基因的盒中c）将盒导入植物细胞中d）分析分离的基因的表达。 本发明还包括用作基因表达调节元件的鉴定序列。