AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS
    7.
    发明申请
    AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS 有权
    DICOT植物的农杆菌介导转化

    公开(公告)号:US20120255070A1

    公开(公告)日:2012-10-04

    申请号:US13438077

    申请日:2012-04-03

    IPC分类号: A01H1/00 C12N15/82 C12N5/04

    CPC分类号: C12N15/8205 C12N1/20

    摘要: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.

    摘要翻译: 提供组合物和方法用于双子叶植物的有效转化。 更具体地,本发明的组合物和方法用于农业用于双子叶植物的农杆菌介导的转化。 组合物包括含有高浓度蔗糖和葡萄糖的培养基。 栽培培养基可用于涉及农杆菌介导的具有感兴趣基因的双子叶植物转化的方法。 以这种方式,可以将任何感兴趣的基因导入具有高转化效率和减少的组织坏死的双子叶植物。

    METHOD FOR TRANSFORMING SOMATIC EMBRYOS
    8.
    发明申请
    METHOD FOR TRANSFORMING SOMATIC EMBRYOS 审中-公开
    用于改变人造胚胎的方法

    公开(公告)号:US20110162111A1

    公开(公告)日:2011-06-30

    申请号:US12976542

    申请日:2010-12-22

    申请人: Theodore M. Klein

    发明人: Theodore M. Klein

    IPC分类号: C12N15/87 C12N15/82 A01H5/00

    CPC分类号: C12N15/8202 C12N15/8206

    摘要: Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.

    摘要翻译: 公开了用目的核酸转化植物细胞并从其再生植物的方法。 该方法对双子叶植物或单子叶植物成熟体细胞胚的转化特别有用。

    Gene Expression Modulating Element

    公开(公告)号:US20100083408A1

    公开(公告)日:2010-04-01

    申请号:US12626962

    申请日:2009-11-30

    CPC分类号: C12N15/8216

    摘要: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.