-
公开(公告)号:US5021348A
公开(公告)日:1991-06-04
申请号:US609677
申请日:1984-05-14
CPC分类号: C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5254 , C12N2770/32434 , C12N2770/32464
摘要: Hepatitis A virus is attenuated by propagation in cell culture in vitro by direct inoculation of the cell cultures with a human clinical specimen containing the virus.
摘要翻译: 通过用含有病毒的人临床标本直接接种细胞培养物,甲型肝炎病毒在细胞培养物中的繁殖减弱。
-
公开(公告)号:US5514376A
公开(公告)日:1996-05-07
申请号:US16486
申请日:1993-02-10
CPC分类号: C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5252 , A61K2039/5254 , A61K2039/575 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464
摘要: Hepatitis A virus is attenuated in virulence by in vitro passage in susceptible cell cultures, without prior passage in a subhuman primate. This process results in a live, attenuated hepatitis A virus vaccine suitable for human disease prevention.
摘要翻译: 甲型肝炎病毒通过在易感细胞培养物中的体外传代而在毒力下减弱,而无需先前在非人灵长类动物中传代。 该过程导致适合人类疾病预防的活的,减毒的甲型肝炎病毒疫苗。
-
公开(公告)号:US5766603A
公开(公告)日:1998-06-16
申请号:US458196
申请日:1995-06-02
CPC分类号: C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5252 , A61K2039/5254 , A61K2039/575 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464
摘要: Hepatitis A virus is attenuated in virulence by in vitro passage in susceptible cell cultures, without prior passage in a subhuman primate. This process results in a live, attenuated hepatitis A virus vaccine suitable for human disease prevention.
-
公开(公告)号:US4783407A
公开(公告)日:1988-11-08
申请号:US781830
申请日:1985-09-30
CPC分类号: C12N7/00 , A61K39/12 , A61K39/29 , C12N2770/32434 , C12N2770/32464
摘要: Hepatitus A virus is grown in Vero cells after passage in in vitro cell culture. Virus replication and continued passage in Vero cells requires extended incubation times, up to about four weeks, for early passages and incubation temperatures no higher than about 32.degree. C. Continued passage results in a significant decrease in incubation time and an increase in virus yield. Cultivation of hepatitus A virus in Vero cells meets the development requirements for an inactivated human vaccine.
摘要翻译: Hepatitus A病毒在体外细胞培养物中通过后在Vero细胞中生长。 Vero细胞中的病毒复制和持续传代需要延长的孵育时间,长达约4周,用于早期传代和孵育温度不高于约32℃。连续传代导致孵育时间的显着降低和病毒产量的增加。 Vero细胞中肝病毒A的培养符合灭活人类疫苗的发展要求。
-
公开(公告)号:US4029764A
公开(公告)日:1977-06-14
申请号:US530623
申请日:1974-12-09
IPC分类号: C12N7/00 , A61K39/29 , G01N33/576 , A61K39/12
CPC分类号: G01N33/5768
摘要: Hepatitis A antigen obtained from the livers of non-human primates infected with hepatitis A (infectious hepatitis) virus has been found to be usable in fast, simple assays for hepatitis A antibody and thus usable in diagnosis of hepatitis A disease in humans, and for the preparation of hepatitis A vaccine.
摘要翻译: 已经发现从感染甲型肝炎(感染性肝炎)病毒的非人灵长类动物的肝脏获得的甲型肝炎抗原可用于甲型肝炎抗体的快速,简单的测定中,因此可用于诊断人类中的甲型肝炎病毒 甲型肝炎疫苗的制备。
-
公开(公告)号:US4164566A
公开(公告)日:1979-08-14
申请号:US934293
申请日:1978-08-17
CPC分类号: A61K39/29 , A61K39/12 , A61K2039/5252 , A61K2039/5254 , C12N2770/32434 , C12N2770/32451
摘要: Hepatitis A virus is passaged at least once in a susceptible sub-human primate; the infected liver from such passage is used to inoculate an in vitro cell culture, the inoculated cell culture is incubated until hepatitis A antigen is detected in the cell sheet or culture fluid, and at least two serial in vitro passages in cell culture are carried out. The hepatitis A virus so modified can be used to prepare live, attenuated hepatitis A vaccine or an inactivated hepatitis A vaccine.
摘要翻译: {PG,1甲型肝炎病毒在易感亚人灵长类动物中传代至少一次; 将来自该传代的受感染的肝脏用于接种体外细胞培养物,孵育接种的细胞培养物直至在细胞片或培养液中检测到甲型肝炎抗原,并进行细胞培养中的至少两次连续体外传代 。 如此修饰的甲型肝炎病毒可用于制备活的,减毒的甲型肝炎疫苗或灭活的甲型肝炎疫苗。
-
公开(公告)号:US4031203A
公开(公告)日:1977-06-21
申请号:US693668
申请日:1976-06-07
IPC分类号: A61K39/29 , G01N33/576 , A61K39/12
CPC分类号: A61K39/29 , A61K39/12 , G01N33/5768 , C12N2770/32434
摘要: Hepatitis A antigen obtained from the livers of non-human primates infected with hepatitis A (infectious hepatitis) virus has been found to be usable in fast, simple assays for hepatitis A antibody and thus usable in diagnosis of hepatitis A disease in humans, and for the preparation of hepatitis A vaccine.
-
公开(公告)号:US4017601A
公开(公告)日:1977-04-12
申请号:US623184
申请日:1975-10-16
IPC分类号: G01N33/576 , A61K39/29 , C12Q1/70 , A61K39/12
CPC分类号: A61K39/29 , A61K39/12 , A61K2039/5252 , A61K2039/575 , C12N2770/32434
摘要: Hepatitis A antigen obtained from the stools of patients infected with hepatitis A (infectious hepatitis) virus has been found to be usable in fast, simple assays for hepatitis A antibody and thus usable in diagnosis of hepatitis A disease, and for the preparation of hepatitis A vaccine.
摘要翻译: 已经发现从感染甲型肝炎(感染性肝炎)病毒的患者的粪便获得的甲型肝炎抗原可用于甲型肝炎抗体的快速,简单的测定中,因此可用于诊断甲型肝炎疾病和用于制备甲型肝炎 疫苗。
-
公开(公告)号:US5997880A
公开(公告)日:1999-12-07
申请号:US419223
申请日:1994-03-18
IPC分类号: C12P21/02 , A61K35/76 , A61K39/00 , A61K39/25 , A61P25/00 , A61P31/12 , A61P31/22 , C12N7/00 , A61K39/245
CPC分类号: A61K39/25 , A61K39/12 , A61K2039/5254 , C12N2710/16734
摘要: Herpes Zoster, or varicella related post herpetic neuralgia is alleviated by immunizing people at risk of developing herpes zoster with varicella zoster virus (VZV) antigen.
摘要翻译: 带状疱疹带状疱疹或水痘相关的疱疹性神经痛可以通过免疫接种水痘带状疱疹病毒(VZV)抗原的带状疱疹风险的人来缓解。
-
公开(公告)号:US5607852A
公开(公告)日:1997-03-04
申请号:US347345
申请日:1994-12-05
IPC分类号: A61K39/00 , A61K39/12 , A61K39/25 , A61P31/12 , A61P31/22 , C12N1/38 , C12N5/00 , C12N5/02 , C12N5/071 , C12N5/10 , C12N7/04 , C12N7/08 , C12N9/84 , C12N7/00 , A61K39/245
CPC分类号: C12N5/0018 , A61K39/12 , A61K39/25 , A61K2039/5254 , C12N2500/36 , C12N2710/16734
摘要: A live, attenuated varicella zoster virus vaccine is produced with enhanced yield of VZV. The new process makes mass production of a live VZV vaccine more practical. In addition, optimized monoloyer cell culture conditions provide a process for maximizing monolayer cell density which is useful for enhancing viral vaccine production. According to this process, cell densities approaching 500,000 cells/cm.sup.2 are routinely achieved in conventional culture vessels.
摘要翻译: PCT No.PCT / US93 / 04986 Sec。 371日期1994年12月5日第 102(e)日期1994年12月5日PCT提交1993年5月26日PCT公布。 公开号WO93 / 24616 PCT 日期:1993年12月9日,生产减毒水痘带状疱疹病毒疫苗,具有提高的VZV产量。 新工艺使大量生产活VZV疫苗更加实用。 此外,优化的单体细胞培养条件提供了最大化单层细胞密度的方法,其可用于增强病毒疫苗生产。 根据该方法,在常规培养容器中常规地实现接近50万个细胞/ cm 2的细胞密度。
-
-
-
-
-
-
-
-
-