公开(公告)号：US20200038839A1

公开(公告)日：2020-02-06

申请号：US16599260

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/28 ,  B01J20/26 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

Abstract: The present disclosure relates to a method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M). Further, the present disclosure relates to particles obtained or obtainable by this method as well as to the use of these particles. In a further aspect, the disclosure relates to a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte.

公开(公告)号：US11724246B2

公开(公告)日：2023-08-15

申请号：US16599260

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/26 ,  B01J20/28 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

CPC classification number: B01J20/28009 ,  B01J20/267 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/3234 ,  B01J20/3272 ,  B01J20/3282 ,  B01J20/3293 ,  B03C1/01 ,  G01N33/54326 ,  B03C2201/18 ,  B03C2201/26

Abstract: A method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M) is disclosed. Further, particles obtained or obtainable by this method as well as to the use of these particles are disclosed. In a further aspect, a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte is disclosed.

公开(公告)号：US20200041502A1

公开(公告)日：2020-02-06

申请号：US16599480

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Jens Christian Bolle ,  Martin Eduardo Silvestre ,  Stephan Hug ,  Carolyn Bylda

IPC: G01N33/543 ,  G01N33/553 ,  B01J13/14 ,  B03C1/01

Abstract: The present disclosure relates to magnetic particles, wherein each particle has a polymer matrix and at least one magnetic core (M), wherein the polymer matrix has at least one crosslinked polymer and wherein the magnetic particle has a particle size in the range of from 1 to 60 micrometer. Further, the present disclosure relates to a method of preparing such particles, and to particles obtainable or obtained by said method. Moreover, the present disclosure uses these magnetic particles for qualitative and/or quantitative determination of at least one analyte in a fluid. Further, the present disclosure relates to a method for determining at least one analyte in a fluid sample using the contacting of a magnetic particle of the disclosure or a magnetic particle obtained by the method of the present disclosure with a fluid sample having or suspected to have the at least one analyte.

公开(公告)号：US20230381743A1

公开(公告)日：2023-11-30

申请号：US18338633

申请日：2023-06-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/28 ,  B01J20/26 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

CPC classification number: B01J20/28009 ,  B01J20/267 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B03C2201/26 ,  B01J20/3272 ,  B03C1/01 ,  G01N33/54326 ,  B01J20/3282 ,  B01J20/3293 ,  B03C2201/18 ,  B01J20/3234

Abstract: The present disclosure relates to a method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M). Further, the present disclosure relates to particles obtained or obtainable by this method as well as to the use of these particles. In a further aspect, the disclosure relates to a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte.

公开(公告)号：US20200348294A1

公开(公告)日：2020-11-05

申请号：US16931599

申请日：2020-07-17

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Gaston Hubertus Maria Vondenhoff ,  Stephan Hug

IPC: G01N33/543 ,  C08F292/00 ,  C08J3/24 ,  C08K3/22 ,  C08G83/00 ,  C08K9/04 ,  C08K5/17 ,  H01F1/00

Abstract: The present disclosure relates to hypercrosslinked magnetic particle with a polymer matrix and at least one magnetic core (M), wherein the polymer matrix has at least one crosslinked polymer having at least one hypercrosslinking bond, wherein the hypercrosslinking bond is a molecule having at least two nitrogen atoms within its structure which are part of the hypercrosslinking bond; and having at least one positive charge. Further, the disclosure relates to a method of preparing the hypercrosslinked magnetic particle and also to hypercrosslinked magnetic particle obtained or obtainable from the method. Also described is the use of the hypercrosslinked magnetic particles for enrichment or purification of at least one analyte as well as to the use of the hypercrosslinked magnetic particles for purification of water.