公开(公告)号：US20090263848A1

公开(公告)日：2009-10-22

申请号：US12227186

申请日：2007-04-24

申请人： Stefan Obermann ,  Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria De Jesus ,  Matthieu Stettler ,  Martin Jordan

发明人： Stefan Obermann ,  Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria De Jesus ,  Matthieu Stettler ,  Martin Jordan

IPC分类号： C12Q1/02

CPC分类号： G01N33/58 ,  G01N1/30 ,  G01N15/042 ,  G01N33/5005 ,  Y10T436/25375

摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently coloured sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.

摘要翻译： 本发明涉及一种用于确定细胞培养物中细胞存活率的方法，所述方法包括以下步骤：根据染色体的活力来染色样品，所述染料可渗透到单个细胞中，确定细胞的比例 彩色到不同的程度。 本发明的区别在于，将着色细胞的细胞悬浮液作为样品在样品容器（10）中离心直到样品中存在的固体含量作为压缩细胞饼（13）沉降， 为了确定比例，压缩细胞饼（13）的不同颜色的部分（131,132,133）的体积被确定为不同存活力的细胞比例的量度。

公开(公告)号：US08107715B2

公开(公告)日：2012-01-31

申请号：US12227184

申请日：2007-04-24

申请人： Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria de Jesus ,  Martin Jordan ,  Matthieu Stettler ,  Stefan Obermann

发明人： Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria de Jesus ,  Martin Jordan ,  Matthieu Stettler ,  Stefan Obermann

IPC分类号： G06K9/00 ,  G06K9/32 ,  G06K9/36

CPC分类号： G01N21/0303 ,  G01N2021/0367

摘要： The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.

摘要翻译： 本发明涉及一种用于检测纵向样品容器（10）中的样品的装置，包括用于将样品容器保持在壳体中的样品容器保持器（30）。 所述样品容器保持器包括在样品容器的至少一个纵向侧区域中的侧视窗和位于样品容器的至少一个正面的区域中的前视窗（35）。 所述装置还包括用于通过前视窗照亮样本容器的第一照明装置，用于通过侧视窗照射样本容器的第二照明装置，以及用于检测通过以下方式照射的样本的第一图像的成像光电检测器： 第一照明装置和通过第二照明装置照亮的样本的第二图像。 所述检测通过侧视窗进行。 所述设备还包括数字数据处理单元，用于覆盖第一和第二图像，并用于在连接到数据处理单元的显示设备上表示所得覆盖图像。

公开(公告)号：US20090103772A1

公开(公告)日：2009-04-23

申请号：US12227184

申请日：2007-04-24

申请人： Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria De Jesus ,  Martin Jordan ,  Matthieu Stettler ,  Stefan Obermann

发明人： Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria De Jesus ,  Martin Jordan ,  Matthieu Stettler ,  Stefan Obermann

IPC分类号： G06K9/00

CPC分类号： G01N21/0303 ,  G01N2021/0367

摘要： The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.

摘要翻译： 本发明涉及一种用于检测纵向样品容器（10）中的样品的装置，包括用于将样品容器保持在壳体中的样品容器保持器（30）。 所述样品容器保持器包括在样品容器的至少一个纵向侧区域中的侧视窗和位于样品容器的至少一个正面的区域中的前视窗（35）。 所述装置还包括用于通过前视窗照亮样本容器的第一照明装置，用于通过侧视窗照射样本容器的第二照明装置，以及用于检测通过以下方式照射的样本的第一图像的成像光电检测器： 第一照明装置和通过第二照明装置照亮的样本的第二图像。 所述检测通过侧视窗进行。 所述设备还包括数字数据处理单元，用于覆盖第一和第二图像，并用于在连接到数据处理单元的显示设备上表示所得覆盖图像。

公开(公告)号：US08093015B2

公开(公告)日：2012-01-10

申请号：US12227186

申请日：2007-04-24

申请人： Stefan Obermann ,  Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria de Jesus ,  Matthieu Stettler ,  Martin Jordan

发明人： Stefan Obermann ,  Reinhard Baumfalk ,  Oscar-Werner Reif ,  Florian Wurm ,  Maria de Jesus ,  Matthieu Stettler ,  Martin Jordan

IPC分类号： C12Q1/02 ,  G01N1/18

CPC分类号： G01N33/58 ,  G01N1/30 ,  G01N15/042 ,  G01N33/5005 ,  Y10T436/25375

摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.

摘要翻译： 本发明涉及一种用于确定细胞培养物中细胞存活率的方法，所述方法包括以下步骤：根据染色体的活力来染色样品，所述染料可以渗透到各个细胞中，确定细胞的比例 彩色到不同的程度。 本发明的区别在于，将着色细胞的细胞悬浮液作为样品在样品容器（10）中离心直到样品中存在的固体含量作为压缩细胞饼（13）沉降， 为了确定比例，压缩细胞饼（13）的不同颜色的部分（131,132,133）的体积被确定为不同存活力的细胞比例的量度。

公开(公告)号：US20090233334A1

公开(公告)日：2009-09-17

申请号：US12046374

申请日：2008-03-11

申请人： Markus Hildinger ,  Florian Wurm ,  Matthieu Stettler ,  Maria DeJesus

发明人： Markus Hildinger ,  Florian Wurm ,  Matthieu Stettler ,  Maria DeJesus

IPC分类号： C12P21/04 ,  C12N7/00 ,  C12N5/04 ,  C12N5/06 ,  C12N5/02 ,  C12N5/08 ,  C12N1/00

CPC分类号： C12P21/02 ,  C12M23/14 ,  C12M27/16 ,  C12M41/02

摘要： The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.

摘要翻译： 本发明提供了一种培养细胞的新方法以及通过大规模培养细胞（达1,500L标称体积和750L工作体积）来生产重组蛋白的新方法，由此充气袋提供无菌的，一次性的 栽培室。 充气袋部分地装有液体培养基和细胞，并放置在容纳容器中。 安全壳位于轨道上摇摆的平台上。 轨道振荡使得容纳容器和袋子移动并引起其运动到其中容纳的液体（“混合”）。 这种运动（由轨道震荡引起）引起动态力场，确保细胞悬浮，本体混合和从液体表面到呼吸细胞的氧传递，而不损害剪切或泡沫产生。