公开(公告)号：US20240010973A1

公开(公告)日：2024-01-11

申请号：US18226877

申请日：2023-07-27

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Sergey VIUKOV ,  Emilie WILDSCHUTZ ,  Noa NOVERSHTERN ,  Carine JOUBRAN ,  Segev NAVEH TASSA ,  Alejandro CASTREJON AGUILERA ,  Bernardo OLDAK ,  Shadi TARAZI ,  Francesco RONCATO

IPC: C12N5/073

CPC classification number: C12N5/0604 ,  C12N2506/03 ,  C12N2501/155 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/91 ,  C12N2500/02

Abstract: Methods of generating a synthetic embryo are provided. Accordingly, there is provided a method of generating a synthetic embryo comprising inducing expression of a factor that induces differentiation to trophectoderm cells in a subpopulation of naïve pluripotent stem cells (PSCs) to obtain a trophectoderm primed cells; inducing expression of a factor that induces differentiation to extra embryonic primitive endodermal cells in a second subpopulation of naïve PSCs to obtain extra embryonic primitive endodermal primed cells; and mixing said trophectoderm primed cells and said extra embryonic primitive endodermal primed cells with naïve PSCs under conditions that allow formation of aggregated cells. Also provided are articles of manufactures, mixtures and aggregates of cells and methods of using same.

公开(公告)号：US20250101375A1

公开(公告)日：2025-03-27

申请号：US18825336

申请日：2024-09-05

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Sergey VIUKOV ,  Emilie WILDSCHUTZ ,  Noa NOVERSHTERN ,  Carine JOUBRAN ,  Segev NAVEH TASSA ,  Alejandro CASTREJON AGUILERA ,  Bernardo OLDAK ,  Shadi TARAZI ,  Francesco RONCATO

IPC: C12N5/073 ,  C12N5/071

Abstract: Methods of generating a synthetic embryo are provided. Accordingly, there is provided a method of generating a synthetic embryo comprising inducing expression of a factor that induces differentiation to trophectoderm cells in a subpopulation of naïve pluripotent stem cells (PSCs) to obtain a trophectoderm primed cells; inducing expression of a factor that induces differentiation to extra embryonic primitive endodermal cells in a second subpopulation of naïve PSCs to obtain extra embryonic primitive endodermal primed cells; and mixing said trophectoderm primed cells and said extra embryonic primitive endodermal primed cells with naïve PSCs under conditions that allow formation of aggregated cells. Also provided are articles of manufactures, mixtures and aggregates of cells and methods of using same.

公开(公告)号：US20210363495A1

公开(公告)日：2021-11-25

申请号：US17382500

申请日：2021-07-22

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Tom Haim SHANI ,  Sergey VIUKOV ,  Rada MASSARWA ,  Jonathan BAYERL ,  Muneef AYYASH

IPC: C12N5/074

Abstract: A culture medium comprising a WNT inhibitor, a SRC inhibitor and a protein kinase C (PKC) inhibitor is disclosed. The medium is devoid of an amount of GSK3beta inhibitor that increases beta-catenin translocation to the nucleus of a pluripotent stem cell being cultured in the culture medium. Uses thereof are also disclosed.

公开(公告)号：US20240318144A1

公开(公告)日：2024-09-26

申请号：US18433553

申请日：2024-02-06

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Yoach RAIS

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

公开(公告)号：US20210253998A1

公开(公告)日：2021-08-19

申请号：US17117157

申请日：2020-12-10

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Yoach RAIS

IPC: C12N5/0735 ,  C12N5/074

Abstract: Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

公开(公告)号：US20170275593A1

公开(公告)日：2017-09-28

申请号：US15500163

申请日：2015-07-30

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Yoach RAIS

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.