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    System for the internal qualitative and quantitative validation of marker indices
    1.
    发明授权
    System for the internal qualitative and quantitative validation of marker indices 有权
    用于内部定性和定量验证标记指标的系统

    公开(公告)号：US07560282B2

    公开(公告)日：2009-07-14

    申请号：US10182013

    申请日：2001-01-23

    申请人： Johannes Gerdes

    发明人： Johannes Gerdes

    IPC分类号： G01N31/00

    CPC分类号： G01N33/57484 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/2525

    摘要： The present invention relates to the qualitative and quantitative validation of marker indices, especially for medical diagnosis and particularly for the determination of the growth fraction in a sample with antibodies against the Ki-67 protein. Diagnostic kit for the quantification of a cell fraction labeled by a marker for in vitro diagnosis, characterized in that a pseudo-tissue is used for the intra- and inter-assay standardization of the marker index.

    摘要翻译： 本发明涉及标记指数的定性和定量验证，特别是用于医学诊断，特别是用于测定具有针对Ki-67蛋白的抗体的样品中生长分数的定性和定量验证。 用于定量由用于体外诊断的标志物标记的细胞级分的诊断试剂盒，其特征在于使用假组织进行标记物指数的标准化和分析。

    System for establishing a sample cover on a substrate
    2.
    发明申请
    System for establishing a sample cover on a substrate 审中-公开
    用于在基板上建立样品盖的系统

    公开(公告)号：US20060166371A1

    公开(公告)日：2006-07-27

    申请号：US11224730

    申请日：2005-09-12

    申请人： Gregory Testa ,  Hans Hug

    发明人： Gregory Testa ,  Hans Hug

    IPC分类号： G01N1/00

    CPC分类号： B01L3/508 ,  B01L7/00 ,  B01L9/52 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/1805 ,  G01N1/31 ,  G01N1/312 ,  Y10T436/25

    摘要： The invention provides a manually operated or automated clamping means to seal a solid support substrate to a solid support cover creating a uniform environmental chamber above a specimen mounted to the support substrate. The clamping means may consist of several components that may act ad a system to provide a repeatable and uniform clamping load to the substrate and cover.

    摘要翻译： 本发明提供了一种手动操作或自动夹紧装置，用于将固体支撑基底密封到固体支撑盖上，在安装到支撑基底上的试样上方产生均匀的环境室。 夹紧装置可以由几个部件组成，这些部件可以作用于系统以向基板和盖提供可重复且均匀的夹紧载荷。

    Method and apparatus for syringe-based sample introduction within a flow cytometer
    6.
    发明申请
    Method and apparatus for syringe-based sample introduction within a flow cytometer 审中-公开
    用于流式细胞仪中基于注射器的样品引入的方法和装置

    公开(公告)号：US20070025879A1

    公开(公告)日：2007-02-01

    申请号：US11190300

    申请日：2005-07-27

    申请人： Angela Vandergaw

    发明人： Angela Vandergaw

    IPC分类号： G01N33/00

    CPC分类号： G01N35/1095 ,  G01N2015/1409

    摘要： An apparatus for introducing a specimen into a flow cytometer comprises: a syringe having a hollow barrel containing the specimen, a plunger partially within the barrel and a needle that extends into a volume of a nozzle of the flow cytometer; a one-way port in the nozzle forming a seal against the needle; a mounting platform coupled to both the syringe and to the flow cytometer; and a syringe pump coupled to the plunger, the syringe pump comprising a motor, a drive mechanism coupled to the motor; and a clamping mechanism coupled to the drive mechanism, wherein the motor operates the drive mechanism so as to cause the clamping mechanism to depress the plunger into the barrel.

    摘要翻译： 用于将样品引入流式细胞仪的装置包括：注射器，其具有容纳所述样本的中空筒体，部分地在所述筒体内的柱塞和延伸到所述流式细胞仪的喷嘴体积中的针; 喷嘴中的单向口形成针头的密封; 耦合到注射器和流式细胞仪两者的安装平台; 以及联接到所述柱塞的注射泵，所述注射器泵包括马达，联接到所述马达的驱动机构; 以及联接到所述驱动机构的夹紧机构，其中所述马达操作所述驱动机构，以使所述夹紧机构将所述柱塞压入所述筒中。

    Method for detecting acid-resistant microorganisms in the stool
    7.
    发明申请
    Method for detecting acid-resistant microorganisms in the stool 失效
    方法检测粪便中的耐酸微生物

    公开(公告)号：US20070009975A1

    公开(公告)日：2007-01-11

    申请号：US11513129

    申请日：2006-08-31

    申请人： Christian Reiter ,  Gerhard Cullman ,  Ulrike Friedrichs ,  Petra Heppner ,  Meret Lakner ,  Achim Ringeis

    发明人： Christian Reiter ,  Gerhard Cullman ,  Ulrike Friedrichs ,  Petra Heppner ,  Meret Lakner ,  Achim Ringeis

    IPC分类号： G01N33/554 ,  G01N33/569

    CPC分类号： G01N33/56922 ,  C07K16/121 ,  G01N2333/205

    摘要： The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism, wherein (a) a stool sample of a mammal is incubated with at least two different monoclonal antibodies, fragments or derivatives thereof or aptamers under conditions allowing a complex formation of antigens of the acid-resistant microorganism with antibodies, fragments or derivatives thereof or the aptamers, and wherein (aa) the first monoclonal antibody or the fragment or the derivative thereof or the first aptamer specifically binds an epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide; (ab) the second monoclonal antibody or the fragment or the derivative thereof or the second aptamer specifically binds an epitope of a second antigen differing from the epitope of the first antigen, which shows at least with some mammals a structure after the intestinal passage that corresponds to the native structure or the structure which a mammal produces antibodies against after being infected or immunised with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide, wherein the parts of the mammals may overlap according to (aa) and (ab) and in total essentially make up the overall number of infected mammals; and (b) the formation of at least one antigen-antibody complex or antigen-aptamer complex according to (aa) or (ab) is detected.

    摘要翻译： 本发明涉及一种用耐酸微生物检测哺乳动物的感染的方法，其中（a）将哺乳动物的粪便样品与至少两种不同的单克隆抗体，其片段或衍生物或适体在允许 用抗体，其片段或衍生物或适配体复合形成抗酸微生物的抗原，并且其中（aa）第一单克隆抗体或其片段或其衍生物或第一适体特异性结合第一抗原的表位， 其至少与一些哺乳动物一起显示出肠道通过后的结构，该结构对应于哺乳动物产生针对抗酸微生物或其提取物或裂解物或蛋白质的免疫后的抗原的天然结构或结构，或 其片段或合成肽; （ab）第二单克隆抗体或其片段或其衍生物或第二适体特异性结合不同于第一抗原表位的第二抗原的表位，其至少显示出一些哺乳动物在肠道通过后对应的结构 哺乳动物产生抗体的抗原结构或结构，用抗酸微生物或其提取物或裂解物或其蛋白质或其片段或合成肽进行感染或免疫之后产生抗体，其中哺乳动物的部分可以 根据（aa）和（ab）重叠，总共基本上构成感染哺乳动物的总数; 和（b）检测根据（aa）或（ab）形成至少一种抗原 - 抗体复合物或抗原 - 适配子复合物。

    Monoclonal antibodies against human protein Mcm3, process for their production, and their use
    9.
    发明申请
    Monoclonal antibodies against human protein Mcm3, process for their production, and their use 审中-公开
    针对人类蛋白质Mcm3的单克隆抗体，其生产过程及其用途

    公开(公告)号：US20040146513A1

    公开(公告)日：2004-07-29

    申请号：US10753747

    申请日：2004-01-08

    申请人： DakoCytomation Denmark A/S

    发明人： Johannes Gerdes ,  Thomas Scholzen ,  Elmar Endl ,  Claudia Wohlenberg ,  Bettina Baron-Luhr ,  Margrit Kernbach ne Hahn ,  Patricia Prilla ,  Johanna Suwinski ,  Rolf Knippers

    IPC分类号： G01N033/574 ,  A61K039/395

    CPC分类号： C07K16/14 ,  A61K2039/505 ,  Y10S435/975

    摘要： The present invention relates to monoclonal antibodies against the human Mcm3 protein, hybridoma cell lines, that produce such antibodies, procedures for the production and their use, pharmaceutical compositions comprising a monoclonal antibody according to the present invention, their use for the prevention and treating of certain diseases and well as methods relating to the prevention and treatment of diseases associated with Mcm3 expression. Monoclonal antibodies according to the invention detect and bind human Mcm3 monospecifically both in immunohistological and immunobiochemical detection systems. The process for the production of these monoclonal antibodies comprises an initial screening of excess hybridoma supernatant with immunobiochemical methods followed by a second screening of positive hybridoma by means of an immunohistochemical method.

    摘要翻译： 本发明涉及针对人Mcm3蛋白的单克隆抗体，产生此类抗体的杂交瘤细胞系，用于制备及其应用的方法，包含本发明的单克隆抗体的药物组合物，其用于预防和治疗 某些疾病以及与预防和治疗与Mcm3相关的疾病相关的方法。 根据本发明的单克隆抗体在免疫组织化学和免疫生物化学检测系统中单独检测并结合人Mcm3。 制备这些单克隆抗体的方法包括用免疫生物化学方法初步筛选过量的杂交瘤上清液，然后通过免疫组织化学方法对阳性杂交瘤进行第二次筛选。