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    Continuous in-vitro evolution
    2.
    发明申请
    Continuous in-vitro evolution 审中-公开
    连续体外进化

    公开(公告)号：US20070048774A1

    公开(公告)日：2007-03-01

    申请号：US11498974

    申请日：2006-08-03

    申请人： Gregory Coia ,  Peter Hudson ,  Peter Iliades ,  Robert Irving

    发明人： Gregory Coia ,  Peter Hudson ,  Peter Iliades ,  Robert Irving

    IPC分类号： C12Q1/68 ,  C12P21/06 ,  C12P19/34 ,  C12N15/86

    CPC分类号： C12N9/127 ,  C07K16/082 ,  C07K16/18 ,  C07K2317/622 ,  C12N15/102 ,  C12N15/1041 ,  C12N15/1058

    摘要： Provided is a method for the mutation, synthesis and selection of a protein of interest, by first incubating a replicable RNA molecule encoding the protein with ribonucleoside triphosphate precursors of RNA and an RNA-directed RNA polymerase, such that the RNA-directed RNA polymerase replicates the RNA molecule but introduces mutations thereby generating a population of mutant RNA molecules. The mutant RNA molecules are then incubated with a translation system under conditions which result in the synthesis of a population of mutant proteins. After translation, the mutant proteins are linked to their encoding RNA molecules, and one or more mutant proteins of interest are selected.

    摘要翻译： 提供了通过首先将编码蛋白质的可复制RNA分子与RNA的核糖核苷三磷酸前体和RNA指导的RNA聚合酶一起孵育来进行突变，合成和选择目的蛋白质的方法，使得RNA指导的RNA聚合酶复制 RNA分子引入突变，从而产生突变型RNA分子群体。 然后将突变体RNA分子与翻译系统在导致合成突变蛋白质群体的条件下孵育。 翻译后，突变蛋白与其编码RNA分子连接，并且选择一种或多种感兴趣的突变蛋白。

    Bifunctional molecules
    3.
    发明授权
    Bifunctional molecules 失效
    双功能分子

    公开(公告)号：US07026446B1

    公开(公告)日：2006-04-11

    申请号：US09581924

    申请日：1998-12-24

    申请人： John Leslie Atwell ,  Peter Leonard Devine ,  Gregory Coia ,  Alexander Andrew Kortt ,  Gillian Wendy Perry ,  Peter Gregory Bundesen

    发明人： John Leslie Atwell ,  Peter Leonard Devine ,  Gregory Coia ,  Alexander Andrew Kortt ,  Gillian Wendy Perry ,  Peter Gregory Bundesen

    IPC分类号： C07K16/46 ,  C07K14/31 ,  C07K14/315 ,  C07K14/435 ,  C07K14/705

    CPC分类号： C07K14/31 ,  C07K14/36 ,  C07K16/10 ,  C07K16/1081 ,  C07K16/18 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2319/00

    摘要： The invention relates to a chimeric antibody conjugate comprising an antigen binding region of a non-human antibody and an immunoglobulin constant region which comprises at least one CH domain or epitope thereof, with the proviso that the constant region is not a naturally occurring FC fragment. A bifunctional molecule for use in labelling an antibody derived from a first species, the bifunctional molecule comprising a binding region which binds to the antibody of the first species or to one or more groups provided thereon, and a constant region derived from an antibody of a second species, the constant region comprising at least one CH domain or an epitope thereof. The present invention relates to bifunctional molecules and complexes which are useful as positive control reagents in antibody based diagnostic tests. The present invention also relates to polynucleotides encoding these bifunctional molecules, and to diagnostic assays involving the use of these molecules.

    摘要翻译： 本发明涉及包含非人抗体的抗原结合区和免疫球蛋白恒定区的嵌合抗体缀合物，其包含至少一个C H H结构域或其表位，条件是恒定区 不是天然存在的F C C片段。 用于标记来自第一物种的抗体的双功能分子，所述双功能分子包含与第一物种的抗体结合的结合区或其上提供的一个或多个基团的结合区和源自 第二种，恒定区包含至少一个C H H结构域或其表位。 本发明涉及在基于抗体的诊断测试中可用作阳性对照试剂的双功能分子和复合物。 本发明还涉及编码这些双功能分子的多核苷酸以及涉及使用这些分子的诊断测定。

    In vivo affinity maturation scheme
    5.
    发明申请
    In vivo affinity maturation scheme 审中-公开
    体内亲和力成熟方案

    公开(公告)号：US20060099611A1

    公开(公告)日：2006-05-11

    申请号：US11156014

    申请日：2005-06-17

    申请人： Robert Irving ,  Peter Hudson ,  Huseyin Mustafa ,  Kim Wark ,  Matias Abregu

    发明人： Robert Irving ,  Peter Hudson ,  Huseyin Mustafa ,  Kim Wark ,  Matias Abregu

    IPC分类号： C12Q1/68 ,  C12P21/06 ,  C12N1/21 ,  C12N1/18 ,  C12N5/06

    CPC分类号： C40B40/02 ,  C12N15/102 ,  C12N15/1037

    摘要： The present invention relates to the field of evolution of nucleic acids in vivo and provides methods and compositions for introducing diversity into gene products. The present invention allows generation of new sequences that have desirable properties by virtue of high frequency mutation events within a cell. The high frequency mutation of a polynucleotide sequence results in, the production of a large population of new sequence variants. Appropriate selection and/or screening permits identification and isolation of mutant forms of the polynucleotide sequence as well as products resulting from expression of the mutant sequences.

    摘要翻译： 本发明涉及体内核酸进化领域，并提供了将多样性引入基因产物的方法和组合物。 本发明允许通过细胞内的高频突变事件产生具有期望特性的新序列。 多核苷酸序列的高频突变导致大量新序列变体的产生。 适当的选择和/或筛选允许鉴定和分离突变形式的多核苷酸序列以及由突变序列的表达产生的产物。