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公开(公告)号：US20140068875A1
公开(公告)日：2014-03-13
申请号：US14116757
申请日：2012-05-10
申请人： Yoshihiko Hirose , Yukihide Sato
发明人： Yoshihiko Hirose , Yukihide Sato
CPC分类号： A61K8/99 , A61K8/66 , A61Q5/10 , C12N9/001 , C12N9/0057 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0071 , C12P13/00 , C12Y103/03005 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y110/03004 , C12Y114/18001 , D06P1/32 , D06P3/14
摘要： The object is to provide a process for dyeing that can provide an excellent dyeing effect while utilizing an oxidase, and a dyeing agent for use in the process. Provided is a dyeing agent comprising a modified enzyme obtained by adding positive charge by a chemical modification with an amine to an enzyme selected from the group consisting of an enzyme specified as EC 1.10.3.1, an enzyme specified as EC 1.10.3.2, an enzyme specified as EC 1.3.3.5, an enzyme specified as EC 1.10.3.4, an enzyme specified as EC 1.10.3.3 and an enzyme specified as EC 1.14.18.1. A process for dyeing using the dyeing agent is also provided.
摘要翻译： 本发明的目的是提供一种可以在使用氧化酶时提供优异的染色效果的染色方法和用于该方法的染色剂。 提供了一种染色剂，其包含通过化学修饰将正电荷与胺相加的酶所选择的酶所选择的酶，所述酶选自指定为EC1.10.3.1的酶，规定为EC1.10.3.2的酶，酶 规定为EC 1.3.3.5，规定为EC 1.10.3.4的酶，指定为EC 1.10.3.3的酶和指定为EC 1.14.18.1的酶。 还提供了使用染色剂进行染色的方法。
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公开(公告)号：US09546363B2
公开(公告)日：2017-01-17
申请号：US14399983
申请日：2013-05-23
发明人： Kenta Kinjo , Tomoko Aratake
CPC分类号： C12N9/96 , C12N9/0063 , C12Q1/26 , C12Y110/03003 , G01N2333/90235
摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.
摘要翻译： 提供：稳定抗坏血酸氧化酶的方法; 一种保存抗坏血酸氧化酶的方法; 和稳定的抗坏血酸氧化酶组合物。 用于稳定抗坏血酸氧化酶的方法和保存抗坏血酸氧化酶的方法，每种方法包括使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存; 和抗坏血酸氧化酶的稳定化组合物，其包含使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存。 用于稳定抗坏血酸氧化酶的方法，保存抗坏血酸氧化酶的方法和本发明的抗坏血酸氧化酶稳定化组合物可用于临床诊断等。
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3.发明授权
公开(公告)号：US4451487A
公开(公告)日：1984-05-29
申请号：US377277
申请日：1982-05-12
申请人： Hellmuth Vetter , Peter Scheibe , Waldemar Thum , Gotthilf Naher
发明人： Hellmuth Vetter , Peter Scheibe , Waldemar Thum , Gotthilf Naher
IPC分类号： A23J1/00 , A61K38/22 , A61K38/43 , A61K38/44 , A61K39/395 , C07K1/30 , C12N9/00 , C12N9/02 , C12N9/08 , C12N9/18 , C12N9/20 , C12P21/00
CPC分类号： C12Y110/03003 , A23J1/00 , C07K1/30 , C12N9/00 , C12N9/0063 , C12N9/0065 , C12N9/18 , C12N9/20 , Y10S435/814
摘要： The present invention provides a process for the purification or enrichment of biologically active proteins by the addition of a selective precipitation agent, wherein an aqueous solution containing the desired protein is mixed at a pH value below its isoelectric point with such an amount of an arylsulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N.dbd.N double bond, that at least the greater part of the biological activity of the desired active protein is present in the precipitate, the precipitate is separated off and the active protein is again brought into solution at a pH value above its isoelectric point.The present invention also provides an agent for the selective precipitation of biologically active proteins from aqueous solutions thereof without the loss of the biological activity, wherein it contains or consists of an aryl sulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N.dbd.N double bond.
摘要翻译： 本发明提供了通过添加选择性沉淀剂来纯化或富集生物活性蛋白质的方法，其中将含有所需蛋白质的水溶液在低于其等电点的pH值下与这样一定量的芳基磺酸 具有两个磺酸根基团的偶氮染料彼此分离，共8个至10个共振关系的双键，其中存在至少一个N = N双键，至少有大部分生物活性 所需的活性蛋白质存在于沉淀物中，分离出沉淀物，并将活性蛋白质再次以高于其等电点的pH值进入溶液中。 本发明还提供了用于从其水溶液中选择性沉淀生物活性蛋白质而不丧失生物活性的试剂，其中它包含或由具有两个磺酸盐基团的芳基磺酸偶氮染料组成，其彼此分离 共有8〜10个双键，其中存在至少一个N = N双键。
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公开(公告)号：US08740994B2
公开(公告)日：2014-06-03
申请号：US14116757
申请日：2012-05-10
申请人： Yoshihiko Hirose , Yukihide Sato
发明人： Yoshihiko Hirose , Yukihide Sato
CPC分类号： A61K8/99 , A61K8/66 , A61Q5/10 , C12N9/001 , C12N9/0057 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0071 , C12P13/00 , C12Y103/03005 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y110/03004 , C12Y114/18001 , D06P1/32 , D06P3/14
摘要： The object is to provide a process for dyeing that can provide an excellent dyeing effect while utilizing an oxidase, and a dyeing agent for use in the process. Provided is a dyeing agent comprising a modified enzyme obtained by adding positive charge by a chemical modification with an amine to an enzyme selected from the group consisting of an enzyme specified as EC 1.10.3.1, an enzyme specified as EC 1.10.3.2, an enzyme specified as EC 1.3.3.5, an enzyme specified as EC 1.10.3.4, an enzyme specified as EC 1.10.3.3 and an enzyme specified as EC 1.14.18.1. A process for dyeing using the dyeing agent is also provided.
摘要翻译： 本发明的目的是提供一种可以在使用氧化酶时提供优异的染色效果的染色方法和用于该方法的染色剂。 提供了一种染色剂，其包含通过化学修饰将正电荷与胺相加的酶所选择的酶所选择的酶，所述酶选自指定为EC1.10.3.1的酶，规定为EC1.10.3.2的酶，酶 规定为EC 1.3.3.5，规定为EC 1.10.3.4的酶，指定为EC 1.10.3.3的酶和指定为EC 1.14.18.1的酶。 还提供了使用染色剂进行染色的方法。
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5.发明授权Ascorbate oxidase isolated from Acremonium sp. HI-25 ferm BP-3124 失效从ACREMONIUM SP分离的抗坏血酸氧化酶。 HI-25 FERM BP-3124
公开(公告)号：US5180672A
公开(公告)日：1993-01-19
申请号：US653709
申请日：1991-02-06
申请人： Homare Itoh , Takashi Shin , Takahisa Yajima , Shigeki Fukuyasu
发明人： Homare Itoh , Takashi Shin , Takahisa Yajima , Shigeki Fukuyasu
IPC分类号： A23C9/12 , A23L2/84 , A23L3/3436 , A23L3/3571 , A23L5/20 , C12N9/02 , C12R1/645
CPC分类号： C12Y110/03003 , A23L3/3571 , C12N9/0063 , Y10S435/822
摘要： An ascorbate oxidase having an optimum pH range of 3.5-4.5 is isolated from Acremonium sp. Hi-25 BP-3124. The ascorbate oxidase is used in foods or drinks to oxidize ascorbate to prevent damage to the foods or drinks by oxidation.
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公开(公告)号：US20170233746A1
公开(公告)日：2017-08-17
申请号：US15503859
申请日：2015-08-14
申请人： DANISCO US INC.
发明人： Susan M. MADRID
CPC分类号： C12N15/52 , C07K14/37 , C12N9/0006 , C12N9/001 , C12N9/0022 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0069 , C12N9/0071 , C12N9/0089 , C12N9/242 , C12Y101/03005 , C12Y101/03009 , C12Y101/99018 , C12Y103/03005 , C12Y104/03002 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y113/11024 , C12Y114/17001 , C12Y114/17003 , C12Y114/18001 , C12Y115/01001
摘要： Aspects of the present disclosure are drawn to methods of improving the expression of secreted cuproenzymes from host cells by manipulating the expression level of one or more proteins involved in copper transport in the host cell, e.g., membrane-bound copper transporting ATPases and soluble copper transporters. The present disclosure also provides compositions containing such improved host cells as well as products derived from the improved host cells that contain one or more cuproenzymes of interest.
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公开(公告)号：US20090226996A1
公开(公告)日：2009-09-10
申请号：US12074432
申请日：2008-03-04
IPC分类号： C12N9/00
CPC分类号： C12N9/0063 , C12N9/0061 , C12Y110/03002 , C12Y110/03003
摘要： A coating composition having an multicopper oxidase enzyme and an oxidizable substrate in combination with an organic binder polymer which may be dispersed or dissolved in a non-aqueous liquid vehicle.
摘要翻译： 一种具有多能氧化酶和可氧化底物与可分散或溶解在非水液体载体中的有机粘合剂聚合物组合的涂料组合物。
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8.发明授权Ascorbate oxidase, gene encoding the same, process for producing the same, and reagent composition using the same 失效抗坏血酸氧化酶，编码其的基因，其制备方法和使用其的试剂组合物
公开(公告)号：US5612208A
公开(公告)日：1997-03-18
申请号：US439114
申请日：1995-05-11
申请人： Yuji Nakanishi , Hitoshi Amano , Shotaro Yamaguchi
发明人： Yuji Nakanishi , Hitoshi Amano , Shotaro Yamaguchi
IPC分类号： A23L3/3571 , C12N9/02 , C12N15/53 , A23J1/00 , C12Q1/26
CPC分类号： C12Y110/03003 , A23L3/3571 , C12N9/0063
摘要： The present invention provides a novel ascorbate oxidase (ASOD) which catalyzes oxidation reaction of L-ascorbic acid with molecular oxygen to form L-dehydroascorbic acid and hydrogen peroxide, a process for producing the ascorbate oxidase comprising using a microorganism belonging to the genus Eupenicillium, a gene encoding ASOD, a transformant containing such a gene, a process for producing ASOD using such a transformant, and a reagent composition comprising ASOD, such as a reagent composition for examination, a food additive, and a reagent composition in the fields of food and clinical examination. The ascorbate oxidase has excellent stability particularly in a liquid state.
摘要翻译： 本发明提供一种新型的抗坏血酸氧化酶（ASOD），其催化L-抗坏血酸与分子氧的氧化反应以形成L-脱氢抗坏血酸和过氧化氢，一种生产抗坏血酸氧化酶的方法，包括使用属于青霉属的微生物， 编码ASOD的基因，含有该基因的转化体，使用这种转化体生产ASOD的方法，以及包含ASOD的试剂组合物，例如食品添加剂和试剂组合物，例如食品中的试剂组合物 和临床检查。 抗坏血酸氧化酶具有优异的稳定性，特别是在液体状态下。
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公开(公告)号：US20150147798A1
公开(公告)日：2015-05-28
申请号：US14399983
申请日：2013-05-23
发明人： Kenta Kinjo , Tomoko Aratake
CPC分类号： C12N9/96 , C12N9/0063 , C12Q1/26 , C12Y110/03003 , G01N2333/90235
摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.
摘要翻译： 提供：稳定抗坏血酸氧化酶的方法; 一种保存抗坏血酸氧化酶的方法; 和稳定的抗坏血酸氧化酶组合物。 用于稳定抗坏血酸氧化酶的方法和保存抗坏血酸氧化酶的方法，每种方法包括使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存; 和抗坏血酸氧化酶的稳定化组合物，其包含使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存。 用于稳定抗坏血酸氧化酶的方法，保存抗坏血酸氧化酶的方法和本发明的抗坏血酸氧化酶稳定化组合物可用于临床诊断等。
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公开(公告)号：US5834273A
公开(公告)日：1998-11-10
申请号：US266097
申请日：1994-06-24
申请人： Masayuki Futatsugi , Kenji Gushi
发明人： Masayuki Futatsugi , Kenji Gushi
CPC分类号： C12Y110/03003 , C12N9/0046 , C12N9/0063 , C12N9/12 , C12N9/78 , C12N9/96
摘要： A modified enzyme obtained by modifying an enzyme such as a hydrogen peroxide-generating enzyme, ascorbate oxidase, etc. with a polysaccharide, polyamino acid or synthetic polymer having a plurality of carboxyl groups via a crosslinking agent capable of binding both carboxyl group and amino group is remarkably improved in resistance to heat, proteases, etc. and in storage stability in an aqueous solution.
摘要翻译： 通过能够结合羧基和氨基的交联剂，通过用多糖，聚氨基酸或具有多个羧基的合成聚合物改性酶，例如产生过氧化氢的酶，抗坏血酸氧化酶等获得的修饰酶 在耐热性，蛋白酶等方面以及在水溶液中的储存稳定性方面显着提高。
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