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公开(公告)号：US09670525B2
公开(公告)日：2017-06-06
申请号：US14420925
申请日：2013-08-30
发明人： Rie Utsugi , Yuki Katayama
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , C12Q1/26 , C12Q1/32 , C12Q1/44 , G01N33/92 , G01N2333/902 , G01N2333/904 , G01N2333/914 , G01N2333/918
摘要： Provided is a method for measuring cholesterol in high-density lipoprotein (HDL) simply and accurately. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising at least one substance selected from the group consisting of pyridinium salts and quaternary ammonium salts each having a specific structure, and a polyanion to form hydrogen peroxide or reduced coenzyme and measuring the formed hydrogen peroxide or reduced coenzyme.
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公开(公告)号：US09663816B2
公开(公告)日：2017-05-30
申请号：US14391885
申请日：2013-04-18
发明人： Hideyuki Kuwata , Tomoko Aratake , Kenta Kinjo
CPC分类号： C12Q1/28 , C09B21/00 , G01N33/581 , G01N33/92
摘要： Provided is a measuring method of a component to be measured in a specimen while suppressing an influence of bilirubin. A measuring method of a component to be measured, comprising converting the component to be measured in the specimen to hydrogen peroxide through an enzymatic reaction, reacting the formed hydrogen peroxide with an oxidative-coloring chromogen in the presence of a peroxidase, and measuring an absorbance of the colored reaction solution to thereby determine the component to be measured, wherein a fatty acid or a salt thereof coexists. The measuring method of a component to be measured in a specimen according to the present invention is useful in clinical diagnosis.
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公开(公告)号：US20160138073A1
公开(公告)日：2016-05-19
申请号：US14898022
申请日：2014-07-07
CPC分类号： C12Q1/26 , C12N9/0006 , C12N9/0032 , C12Q1/37 , C12Y105/03 , G01N33/723 , G01N33/725 , G01N2333/805 , G01N2333/90672 , G01N2333/952
摘要： The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.
摘要翻译： 本发明提供了包含氨基酸序列的蛋白质，其中将包含SEQ ID NO：1所示氨基酸序列的蛋白质的61位精氨酸取代为选自甘氨酸，丙氨酸，缬氨酸， 亮氨酸，丝氨酸，苏氨酸，脯氨酸，半胱氨酸，甲硫氨酸，天冬酰胺，谷氨酰胺和天冬氨酸; 以及测定样品中的糖化血红蛋白的方法，其中所述方法包括使样品中的糖化血红蛋白与蛋白酶反应以产生糖化六肽，然后使产生的糖基化六肽与前述蛋白质反应，并测量产生或消耗的物质 通过反应。
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公开(公告)号：US08790905B2
公开(公告)日：2014-07-29
申请号：US13633410
申请日：2012-10-02
发明人： Kazuo Aisaka , Keiko Suzuki
CPC分类号： C12Q1/28 , C07K14/805 , C12N9/0022 , C12N9/0032 , C12P21/005 , C12Q1/26 , C12Q1/37 , G01N33/6893 , G01N2333/9065 , G01N2400/00 , G01N2800/042
摘要： The present invention encompasses: [1] a DNA encoding the protein of any one of (i) a protein comprising the sequence of SEQ ID NO:1; (ii) a protein comprising a sequence with one to ten amino acid deletions, substitutions, or additions in the sequence of SEQ ID NO:1, and having fructosyl peptide oxidase activity; (iii) a protein comprising a sequence having 99% or higher homology to the sequence of SEQ ID NO:1, and having fructosyl peptide oxidase activity; and (iv) a protein having fructosyl peptide oxidase activity, which is encoded by an expression plasmid harbored by the strain deposited under Accession No. FERM BP-11026; [2] a DNA comprising the of SEQ ID NO: 2; and [3] a DNA that hybridizes under stringent conditions with a DNA comprising a sequence complementary to SEQ ID NO: 2, where the DNA encodes a protein having fructosyl peptide oxidase activity.
摘要翻译： 本发明包括：[1]编码下述蛋白质的DNA：（i）包含SEQ ID NO：1的序列的蛋白质; （ii）包含在SEQ ID NO：1的序列中具有1至10个氨基酸缺失，取代或添加的序列并具有果糖基肽氧化酶活性的序列的蛋白质; （iii）包含与SEQ ID NO：1的序列具有99％或更高同源性并具有果糖基氧化酶活性的序列的蛋白质; 和（iv）具有果糖基氧化酶活性的蛋白质，其由以保藏号FERM BP-11026保藏的菌株携带的表达质粒编码; [2]包含SEQ ID NO：2的序列的DNA; 和[3]在严格条件下与包含与SEQ ID NO：2互补的序列的DNA杂交的DNA，其中DNA编码具有果糖基肽氧化酶活性的蛋白质。
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5.发明申请Method and reagent for determination of cholesterol in remnant-like particles 有权用于测定残留物颗粒中胆固醇的方法和试剂公开(公告)号：US20030207342A1
公开(公告)日：2003-11-06
申请号：US10430231
申请日：2003-05-06
发明人： Kazuhito Miyauchi
IPC分类号： C12Q001/60
摘要： The present invention provides a method and a reagent for the simple and sensitive determination of cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase in the presence of oxygen or an oxidized coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme. The reagent comprises (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase.
摘要翻译： 本发明提供了用于简单且灵敏地测定生物样品中残留样颗粒中的胆固醇而不分离样品组分的方法和试剂。 该方法包括使样品与（i）胆固醇酯酶，（ii）胆固醇氧化酶或胆固醇脱氢酶接触，和（iii）在氧或氧化型辅酶存在下的磷脂酶，并测量形成的过氧化氢或还原型辅酶。 试剂包含（i）胆固醇酯酶，（ii）胆固醇氧化酶或胆固醇脱氢酶，和（iii）磷脂酶。
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公开(公告)号：US10859583B2
公开(公告)日：2020-12-08
申请号：US16060518
申请日：2016-12-13
发明人： Yasumasa Shimada , Kohei Tsuchiya , Yuki Kaname
摘要： A feces sampling container having an increased storage stability of a component in a feces sample such as hemoglobin to enable the measurement of a component in a feces sample with a small amount of feces collected. The container includes a container body, and a feces sampling stick having a gripping part on one side and a stick part on the other side, the stick part having a feces sampling part in the vicinity of the tip, wherein the container body comprises an opening part through which the feces sampling part of the feces sampling stick is inserted, and a feces container chamber in which a desiccant is enclosed, wherein the feces sample held by the feces sampling part is dried by a contact of the feces sampling part, which is inserted through the opening part and holding the feces sample, with the desiccant.
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公开(公告)号：US20190177548A1
公开(公告)日：2019-06-13
申请号：US16320343
申请日：2017-07-28
发明人： Haruyo Soya , Yuki Katayama
摘要： The present invention provides a method for preserving a leuco chromogen-containing aqueous solution comprising: adding at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound to a leuco chromogen-containing aqueous solution; a method for stabilizing a leuco chromogen in an aqueous solution comprising: adding at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound to a leuco chromogen-containing aqueous solution; and a liquid reagent comprising a leuco chromogen and at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound.
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公开(公告)号：US09944970B2
公开(公告)日：2018-04-17
申请号：US14898022
申请日：2014-07-07
CPC分类号： C12Q1/26 , C12N9/0006 , C12N9/0032 , C12Q1/37 , C12Y105/03 , G01N33/723 , G01N33/725 , G01N2333/805 , G01N2333/90672 , G01N2333/952
摘要： The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.
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公开(公告)号：US09546363B2
公开(公告)日：2017-01-17
申请号：US14399983
申请日：2013-05-23
发明人： Kenta Kinjo , Tomoko Aratake
CPC分类号： C12N9/96 , C12N9/0063 , C12Q1/26 , C12Y110/03003 , G01N2333/90235
摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.
摘要翻译： 提供：稳定抗坏血酸氧化酶的方法; 一种保存抗坏血酸氧化酶的方法; 和稳定的抗坏血酸氧化酶组合物。 用于稳定抗坏血酸氧化酶的方法和保存抗坏血酸氧化酶的方法，每种方法包括使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存; 和抗坏血酸氧化酶的稳定化组合物，其包含使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存。 用于稳定抗坏血酸氧化酶的方法，保存抗坏血酸氧化酶的方法和本发明的抗坏血酸氧化酶稳定化组合物可用于临床诊断等。
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公开(公告)号：US08883142B2
公开(公告)日：2014-11-11
申请号：US14267729
申请日：2014-05-01
发明人： Kazuo Aisaka , Keiko Suzuki
IPC分类号： C12N9/02 , A61K38/44 , C12Q1/28 , C07K14/805 , C12N9/06 , C12P21/00 , C12Q1/26 , G01N33/68 , C12Q1/37
CPC分类号： C12Q1/28 , C07K14/805 , C12N9/0022 , C12N9/0032 , C12P21/005 , C12Q1/26 , C12Q1/37 , G01N33/6893 , G01N2333/9065 , G01N2400/00 , G01N2800/042
摘要： A reagent measuring a glycated protein, comprising a protease and a protein of any one of [1] to [4] below: [1] a protein comprising the amino acid sequence represented by SEQ ID NO:1; [2] a protein comprising an amino acid sequence with one to ten amino acid deletions, substitutions, or additions in the amino acid sequence represented by SEQ ID NO:1, and having fructosyl peptide oxidase activity; [3] a protein comprising an amino acid sequence having 99% or higher homology to the amino acid sequence represented by SEQ ID NO:1, and having fructosyl peptide oxidase activity; and [4] a protein having fructosyl peptide oxidase activity, which is encoded by an expression plasmid harbored by the Escherichia coli XL1-Blue MRF′ strain deposited under Accession No. FERM BP-11026.
摘要翻译： 测定糖化蛋白质的试剂，其包含下述[1]〜[4]中任一项所述的蛋白酶和蛋白质。[1]一种蛋白质，其包含SEQ ID NO：1所示的氨基酸序列; [2]一种蛋白质，其包含在SEQ ID NO：1所示的氨基酸序列中具有1至10个氨基酸缺失，取代或添加的氨基酸序列，并具有果糖基氧化酶活性; [3]一种蛋白质，其包含与SEQ ID NO：1所示的氨基酸序列具有99％以上同源性的氨基酸序列，并具有果糖基氧化酶活性; 和[4]具有果糖基氧化酶活性的蛋白质，其由以保藏号FERM BP-11026保藏的大肠杆菌XL1-Blue MRF'菌株所携带的表达质粒编码。
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