摘要:
A method of arranging a large number of living bodies, such as cells, embryos, or organisms rapidly, individually, and one by one, a holding sheet for the method, and a device for processing the living bodies. The method of holding living bodies includes using a sheet in which multiple through-holes with a size capable of holding one of the target living bodies, but not capable of holding two or more of the living bodies, are provided, to thereby arrange and hold the living bodies one by one in the multiple through-holes in the sheet together with a liquid.
摘要:
Optical contact liquid containing an amphipathic molecule is dripped onto a semiconductor device which is a sample as an inspection object (S104), and a solid immersion lens is set thereon (S105). The inserted position of the solid immersion lens is then adjusted (S106). The optical contact liquid is then dried (S108), and thereby the solid immersion lens is brought into optically-close contact with the semiconductor device. As a result, a sample observation method and a microscope or the like can be realized, in which the solid immersion lens can be easily aligned to a desired position on the sample, and the solid immersion lens can be securely brought into optically-close contact with the sample.
摘要:
For a semiconductor device S as a sample of an observed object, there are provided an image acquisition part 1 for carrying out observation of the semiconductor device S, and an optical system 2 comprising an objective lens 20. A solid immersion lens (SIL) 3 for magnifying an image of the semiconductor device S is arranged movable between an insertion position where the solid immersion lens includes an optical axis from the semiconductor device S to the objective lens 20 and is in close contact with a surface of the semiconductor device S, and a standby position off the optical axis. Then an image containing reflected light from SIL 3 is acquired with the SIL 3 at the insertion position, and the insertion position of SIL 3 is adjusted by SIL driver 30, with reference to the image. This realizes a semiconductor inspection apparatus (microscope) capable of readily performing observation of the sample necessary for an analysis of microstructure of a semiconductor device or the like, and a semiconductor inspection method (sample observation method) therewith.
摘要:
A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. Selecting an immersion liquid, which equals the refractive index of the near surface tissue, minimizing wavefront distortion which may result from an effectively corrugated surface of the specimen.
摘要:
A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system, especially a laser scanning confocal microscope system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. A stabilizing plate is attached to a surface of the bag which faces the window. An objective lens of the confocal imaging system receives and supports the plate, preferably by a magnetic coupling ring around the plate which is magnetically attached to the barrel of the objective lens. The lens may be fixed and a mechanism for positioning the tray with respect to the lens in a direction along the optical axis of the lens and in orthogonal directions perpendicular to that axis shares a common support with the lens. Wavefront distortion which may result from an effectively corrugated surface of the specimen is minimized by selecting an immersion liquid which equals the refractive index of the near surface tissues. The tissue determines the index of the immersion fluid. The thicknesses and refractive indices of the window and plate are then determined to correct the residual aberration of the optical system. Different trays having different window and plate thicknesses and indices may be provided for different specimen species such as kidney, liver, cervix, et cetera. Thus, a generic confocal imaging system with a generic objective lens is corrected for image aberration and wavefront distortion due to the surface corrugations of the tissue. The correction is simplified since the bag is compliant and compresses so that the optical thickness along the axis between the objective lens and the focal plane in or at the specimen is substantially invariant whether the beam is focused at the interface between the specimen and the window or within the specimen over a focusing range which can extend over several millimeters.
摘要:
Provided is a microscope including: a chamber storing a solution in which a cuvette accommodating a solution together with a sample is immersed and that has an index of refraction identical to that of the solution; an immersion objective lens being placed outside the chamber and collecting light from the sample; a camera acquiring an image of the light collected by the lens; a targeting section moving the lens in a direction along a detection light axis thereof; and a movable stage supporting the cuvette in the chamber so as to be movable in at least a direction along the detection light axis. Each of the cuvette and the chamber has a transparent section that can transmit light coming from the sample. The lens is placed so as to face the transparent section of the cuvette with the transparent section of the chamber interposed therebetween.
摘要:
The application provides a method for reducing the loss of electromagnetic radiation caused by passing electromagnetic radiation through material with a rough surface. The method comprises applying a surface layer, such as a liquid or plastic film, to the material. The surface layer masks or decreases the surface irregularities or effects therefrom resulting in decreased loss of electromagnetic energy.
摘要:
A method of determining the position of inclusions in a gemstone, comprising:(a) placing the gemstone within a material having a refractive index within 0.5, optionally 0.2 or 0. 1, of that of the gemstone;(b) illuminating the gemstone and imaging the illuminated gemstone; and(c) determining the position of inclusions based on images of the inclusions in the images.
摘要:
For a semiconductor device S as a sample of an observed object, there are provided an image acquisition part 1 for carrying out observation of the semiconductor device S, and an optical system 2 comprising an objective lens 20. A solid immersion lens (SIL) 3 for magnifying an image of the semiconductor device S is arranged movable between an insertion position where the solid immersion lens includes an optical axis from the semiconductor device S to the objective lens 20 and is in close contact with a surface of the semiconductor device S, and a standby position off the optical axis. Then an image containing reflected light from SIL 3 is acquired with the SIL 3 at the insertion position, and the insertion position of SIL 3 is adjusted by SIL driver 30, with reference to the image. This realizes a semiconductor inspection apparatus (microscope) capable of readily performing observation of the sample necessary for an analysis of microstructure of a semiconductor device or the like, and a semiconductor inspection method (sample observation method) therewith.
摘要:
A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system, especially a laser scanning confocal microscope system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. A stabilizing plate is attached to a surface of the bag which faces the window. Selecting an immersion liquid, which equals the refractive index of the near surface tissues, minimizes wavefront distortion which may result from an effectively corrugated surface of the specimen.