公开(公告)号：US20070117190A1

公开(公告)日：2007-05-24

申请号：US11601564

申请日：2006-11-16

Applicant: Howard Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Zhu

Inventor： Howard Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C12N9/10 ,  C07H21/04 ,  C12N15/74 ,  C12N1/18

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸[18:2，LA]转化成二十碳烯酸[20：2，EDA]的能力的Δ9延伸酶。 公开了分离的核酸片段和包含编码Δ9延伸酶的这种片段的重组构建物以及在植物和油质酵母中使用这些Delta9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US07067302B2

公开(公告)日：2006-06-27

申请号：US10621826

申请日：2003-07-17

Applicant: Lixuan Huang ,  Zhixiong Xue

Inventor： Lixuan Huang ,  Zhixiong Xue

IPC: C12N15/63 ,  C12N9/88 ,  C07H21/04

CPC classification number: C12Y403/01005 ,  C12N9/88

Abstract: A novel tyrosine ammonia lyase enzyme was identified in the bacterium Rhodobacter sphaeroides. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was cloned by direct amplification using the genomic DNA and was expressed in E. coli.

Abstract translation: 在细菌Rhodobacter sphaeroides中鉴定出新的酪氨酸氨裂解酶。 该酶对酪氨酸的活性高于苯丙氨酸，可用于直接从酪氨酸生产对羟基肉桂酸。 通过使用基因组DNA的直接扩增克隆编码该酶的基因，并在大肠杆菌中表达。

公开(公告)号：US20060094102A1

公开(公告)日：2006-05-04

申请号：US11185301

申请日：2005-07-20

Applicant: Zhixiong Xue ,  Quinn Zhu

Inventor： Zhixiong Xue ,  Quinn Zhu

IPC: C12N9/26 ,  C12N15/74 ,  C12N1/18

CPC classification number: C12N15/81

Abstract: The promoter region associated with the Yarrowia lipolytica ammonium transporter (yat1) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be advantageously inducible under oleaginous conditions (i.e., nitrogen limitation) and are useful to drive expression of genes involved in the production of ω-3 and ω-6 fatty acids.

Abstract translation: 已经发现与解脂耶氏酵母转氨酶（yat1）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已显示本发明的启动子区域在油性条件（即氮限制）下是有利的可诱导的，并且可用于驱动参与产生ω-3和ω-6脂肪酸的基因的表达。

公开(公告)号：US20060094087A1

公开(公告)日：2006-05-04

申请号：US11024545

申请日：2004-12-29

Applicant: Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

Inventor： Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

IPC: C12P7/64 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

CPC classification number: C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

Abstract: An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

Abstract translation: 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

公开(公告)号：US20060029743A1

公开(公告)日：2006-02-09

申请号：US11100295

申请日：2005-04-06

Applicant: Zhixiong Xue

Inventor： Zhixiong Xue

IPC: B05D3/00 ,  C09D5/14 ,  C23C18/00

CPC classification number: C09D5/14 ,  A01N55/00 ,  B05D7/06 ,  B27K3/343 ,  B27K3/40 ,  B27K3/50 ,  A01N43/32 ,  A01N43/84 ,  A01N47/18 ,  A01N2300/00

Abstract: This invention provides a method for preserving wood using a substantially metal-free wood preservative comprising a sodium-channel blocking insecticide and at least one fungicide selected from the group consisting of conazoles and ergosterol biosynthesis inhibitors. This invention also relates to such wood preservative formulations.

Abstract translation: 本发明提供了一种使用基本上无金属的木材防腐剂来保存木材的方法，所述木材防腐剂包含钠通道阻断杀虫剂和至少一种选自由咪唑啉酮和麦角固醇生物合成抑制剂组成的组的杀真菌剂。 本发明还涉及这种木材防腐剂制剂。

公开(公告)号：US20050227956A1

公开(公告)日：2005-10-13

申请号：US10823234

申请日：2004-04-13

Applicant: Ying Wang ,  Zhixiong Xue

Inventor： Ying Wang ,  Zhixiong Xue

IPC: A01N43/54 ,  A01N43/64 ,  A01N43/653 ,  A01N43/78 ,  A01N55/02 ,  A01N59/20

CPC classification number: A01N43/653 ,  A01N59/20 ,  B27K3/22 ,  B27K3/34 ,  B27K3/343 ,  B27K3/52 ,  Y10T428/249925 ,  A01N59/04 ,  A01N43/78 ,  A01N43/54 ,  A01N33/12 ,  A01N33/08 ,  A01N33/04 ,  A01N2300/00

Abstract: A composition comprising a copper compound, a quaternary ammonium compound, an alkanolamine or ammonia, a carbonate, and at least two moldicides wherein the total concentration of moldicides is a maximum of 80 mg per liter, said composition effective to prevent mold growth in pressure treated wood.

Abstract translation: 包含铜化合物，季铵化合物，链烷醇胺或氨，碳酸盐和至少两种杀生物剂的组合物，其中杀生物剂的总浓度最大为每升80mg，所述组合物有效地防止压力处理中的霉菌生长 木。