公开(公告)号：US20060110806A1

公开(公告)日：2006-05-25

申请号：US11264737

申请日：2005-11-01

Applicant: Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  James Ragghianti ,  John Seip ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

Inventor： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  James Ragghianti ,  John Seip ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC: C07H21/04 ,  C12P7/64 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

公开(公告)号：US20070031951A1

公开(公告)日：2007-02-08

申请号：US11436160

申请日：2006-05-17

Applicant: Lixuan Huang ,  Zhixiong Xue ,  Quinn Zhu

Inventor： Lixuan Huang ,  Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/22 ,  C12N9/88 ,  C12N1/21 ,  C12N15/74

CPC classification number: C12N9/1029 ,  C12P7/22

Abstract: A method to produce resveratrol in a recombinant bacterial host cell is provided. Expression of a resveratrol synthase gene in combination with genes involved in the phenylpropanoid pathway enabled recombinant microbial production of resveratrol.

Abstract translation: 提供了在重组细菌宿主细胞中产生白藜芦醇的方法。 白藜芦醇合酶基因与参与苯丙素途径的基因组合的表达使白藜芦醇的重组微生物产生。

公开(公告)号：US20060115881A1

公开(公告)日：2006-06-01

申请号：US11265761

申请日：2005-11-02

Applicant: Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

Inventor： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC: C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

公开(公告)号：US20060094092A1

公开(公告)日：2006-05-04

申请号：US11264784

申请日：2005-11-01

Applicant: Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

Inventor： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC: C12P7/64 ,  C12N15/74 ,  C12N1/18

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

公开(公告)号：US20060057690A1

公开(公告)日：2006-03-16

申请号：US11225354

申请日：2005-09-13

Applicant: Zhixiong Xue ,  Quinn Zhu

Inventor： Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C12N1/18 ,  C12N15/74

CPC classification number: C12N15/815 ,  C12N9/1029 ,  C12P7/6427 ,  C12P21/02 ,  C12Y203/01015

Abstract: The promoter region associated with the Yarrowia lipolytica glycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.

Abstract translation: 已经发现与解脂耶氏酵母甘油-3-磷酸O-酰基转移酶（gpat）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已经显示本发明的启动子区域适合于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。

公开(公告)号：US20070249026A1

公开(公告)日：2007-10-25

申请号：US11787772

申请日：2007-04-18

Applicant: Zhixiong Xue ,  Quinn Zhu

Inventor： Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N1/18

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N9/0087 ,  C12N15/8247 ,  C12P7/6472

Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in plants and oleaginous yeast is disclosed.

Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成它们的ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物，以及使用植物中的这些Delta17去饱和酶和含油酵母制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20070292924A1

公开(公告)日：2007-12-20

申请号：US11748629

申请日：2007-05-15

Applicant: Howard Damude ,  Dana Pollak ,  Zhixiong Xue ,  Quinn Zhu

Inventor： Howard Damude ,  Dana Pollak ,  Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C07H21/04 ,  C12N1/15 ,  C12N9/02

CPC classification number: A23L1/182 ,  A23K10/30 ,  A23L7/196 ,  A23L19/03 ,  A23V2002/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19

Abstract: The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.

公开(公告)号：US20070118929A1

公开(公告)日：2007-05-24

申请号：US11601563

申请日：2006-11-16

Applicant: Howard Damude ,  Brian McGonigle ,  Quinn Zhu ,  Zhixiong Xue

Inventor： Howard Damude ,  Brian McGonigle ,  Quinn Zhu ,  Zhixiong Xue

IPC: A01H5/00 ,  C11B1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20070087420A1

公开(公告)日：2007-04-19

申请号：US11253882

申请日：2005-10-19

Applicant: Daniel MaCool ,  Zhixiong Xue ,  Quinn Zhu

Inventor： Daniel MaCool ,  Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

CPC classification number: C12N9/1029 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to a fungal C16/18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C16/18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16/18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.

Abstract translation: 本发明涉及能够催化棕榈酸酯（16:0）转化为硬脂酸（18:0）的真菌C 16/18脂肪酸延伸酶。 具体地说，提供了被孢霉烯C 16/18脂肪酸延伸酶的核苷酸序列（命名为“ELO3”）。 本文描述了增加微生物油生产，增加碳通量进入多不饱和脂肪酸生物合成途径并通过过度表达C 16/1618脂肪酸延长酶增加多不饱和脂肪酸的含量的方法。 最希望的是，即时ELO3的底物特异性对于能够在含油酵母，如解脂耶氏酵母（Yarrowia lipolytica）中积累长链多不饱和脂肪酸是特别有用的。

公开(公告)号：US20070117190A1

公开(公告)日：2007-05-24

申请号：US11601564

申请日：2006-11-16

Applicant: Howard Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Zhu

Inventor： Howard Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Zhu

IPC: C12P7/64 ,  C12N9/10 ,  C07H21/04 ,  C12N15/74 ,  C12N1/18

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸[18:2，LA]转化成二十碳烯酸[20：2，EDA]的能力的Δ9延伸酶。 公开了分离的核酸片段和包含编码Δ9延伸酶的这种片段的重组构建物以及在植物和油质酵母中使用这些Delta9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。