公开(公告)号：US5958728A

公开(公告)日：1999-09-28

申请号：US972661

申请日：1997-11-18

申请人： Alan Sloma ,  David Sternberg ,  Lee F. Adams ,  Stephen Brown

发明人： Alan Sloma ,  David Sternberg ,  Lee F. Adams ,  Stephen Brown

IPC分类号： C12N1/21 ,  C12N15/75 ,  C12P21/00

CPC分类号： C12N15/75

摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a Bacillus cell, wherein the mutant (i) comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of a surfactin or isoform thereof under conditions conducive for the production of the polypeptide and (ii) the mutant produces less of the surfactin or isoform thereof than the Bacillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.

摘要翻译： 本发明涉及产生多肽的方法，包括：（a）培养芽孢杆菌细胞的突变体，其中所述突变体（i）包含编码所述多肽的第一核酸序列和第二核酸序列，所述第二核酸序列包含 在有利于产生多肽的条件下，负责生物合成或分泌表面活化素或其同种型的基因中的至少一个和（ii）当在相同条件下培养时，突变体产生比芽孢杆菌细胞更少的表面活性素或同种型 ; 和（b）从培养基中分离多肽。 本发明还涉及芽孢杆菌细胞的突变体和产生突变体的方法。

公开(公告)号：US5792014A

公开(公告)日：1998-08-11

申请号：US844130

申请日：1997-04-18

申请人： Stephen Brown

发明人： Stephen Brown

IPC分类号： A63B69/00

CPC分类号： A63B69/0079 ,  A63B69/0095

摘要： A volleyball practice device and method that enables a player to practice spiking a volleyball. The device includes a tether with an attachment device at one end that enables the tether be attached to a regulation play volleyball. The tether is suspended from a support bracket that can be attached to a basketball hoop. The tether is used to hoist the volleyball to a height that is appropriate to practice spiking. The tether is then placed in a quick release mechanism, wherein the weight of the suspended volleyball keeps the tether taut in between the volleyball and the quick release mechanism. As the volleyball is spiked, the tether detaches from the quick release mechanism. The volleyball therefore travels in the direction that it was spiked without being significantly hampered by the attached tether. The tail end of the tether is anchored. After the volleyball travels a predetermined distance, the tether pulls taut and the volleyball is stopped. The volleyball can then be returned to its hoisted position by the tether and readied for another practice spike.

摘要翻译： 一种排球练习装置和方法，使得玩家能够练习排球。 该装置包括在一端具有附接装置的系绳，其能够将系绳连接到调节排球。 系绳悬挂在支架上，可以连接到篮球架上。 系绳用于将排球提升到适合练习加注的高度。 然后将系绳放置在快速释放机构中，其中悬挂的排球的重量将系绳拉紧在排球和快速释放机构之间。 随着排球的飙升，系绳从快速释放机构脱离。 因此，排球沿着被加注的方向行进，而不受连接系绳的显着阻碍。 系链的尾端被锚定。 在排球行进预定距离后，系绳拉紧，排球停止。 然后，排球可以通过系绳返回到其悬挂位置，并准备另一个练习钉。

公开(公告)号：US08700069B2

公开(公告)日：2014-04-15

申请号：US11887949

申请日：2006-04-10

申请人： Malcolm MacNaughtan ,  Christopher Ridgway Drane ,  Stephen Brown ,  Craig Andrew Scott

发明人： Malcolm MacNaughtan ,  Christopher Ridgway Drane ,  Stephen Brown ,  Craig Andrew Scott

IPC分类号： H04W24/00

CPC分类号： H04W64/00 ,  G01S5/0036 ,  G01S5/0252 ,  G01S5/14

摘要： A method and system for calculating the location of a mobile radio terminal in a radio communications network. The method provides for the collection of measurements of radio signal parameters at the mobile radio terminal which are then processed and transmitted to a network processor for calculating the location of the mobile radio terminal. The collected measurements may be filtered and subsets of the measurements may also be selected according to their suitability for particular applications.

摘要翻译： 一种用于计算无线电通信网络中的移动无线电终端的位置的方法和系统。 该方法提供了在移动无线电终端处收集无线电信号参数的测量值，然后将其处理并发送到网络处理器以计算移动无线电终端的位置。 可以过滤收集的测量值，也可以根据其对特定应用的适用性来选择测量的子集。

公开(公告)号：US08518047B2

公开(公告)日：2013-08-27

申请号：US12849476

申请日：2010-08-03

申请人： Robert Metzger ,  Stephen Brown ,  Garrett A. Sheffer

发明人： Robert Metzger ,  Stephen Brown ,  Garrett A. Sheffer

IPC分类号： A61B17/56

CPC分类号： A61B17/155 ,  A61B17/02 ,  A61B17/14 ,  A61B17/142 ,  A61B17/157

摘要： Various method and apparatuses may be used to perform a procedure, such as a resection of a portion of the anatomy for preparation of the implants of a prosthetic. Various resecting member, including saw blades having selected geometries and shapes, can be used to assist in the resection of an anatomy to provide for implantation of a prosthetic. In addition, a cutting block assembly may be used to guide the resecting member. The cutting block assembly may be moveably mounted relative to the portion to be resected.

摘要翻译： 可以使用各种方法和装置来执行手术，例如切除解剖结构的一部分以准备假体的植入物。 可以使用包括具有选定几何形状和形状的锯片的各种切除构件来辅助切除解剖结构以提供假体植入。 此外，可以使用切割块组件来引导切除部件。 切割块组件可相对于要切除的部分可移动地安装。

公开(公告)号：US08463285B2

公开(公告)日：2013-06-11

申请号：US11887948

申请日：2006-04-10

申请人： Malcolm MacNaughtan ,  Christopher Ridgway Drane ,  Craig Andrew Scott ,  Stephen Brown

发明人： Malcolm MacNaughtan ,  Christopher Ridgway Drane ,  Craig Andrew Scott ,  Stephen Brown

IPC分类号： H04W24/00

CPC分类号： H04W64/00 ,  G01S5/0036 ,  G01S5/0252 ,  G01S5/14

摘要： Methods and systems for determining whether a mobile radio terminal is on or out of a zone in a radio communications network. The methods and systems provide for generating a profile representative of the zone using measurements taken by the mobile radio terminal and/or the network and/or using predicted values. A comparison is then made between subsequent measurements or values and the profile to make a determination as to whether the mobile radio terminal is in or out of the zone. The methods and systems are applicable to any zone-based application including differential call charging, security applications, buddy finder services, mobile gaming and location based services in general.

摘要翻译： 用于确定移动无线电终端是否在无线电通信网络中的区域之外的方法和系统。 所述方法和系统提供使用由移动无线电终端和/或网络进行的测量和/或使用预测值来生成代表该区域的简档。 然后在随后的测量或值和简档之间进行比较，以确定移动无线电终端是否在区域内或外。 方法和系统适用于任何基于区域的应用，包括差分呼叫计费，安全应用，好友查找器服务，移动游戏和基于位置的服务。

公开(公告)号：US20120168310A1

公开(公告)日：2012-07-05

申请号：US13379585

申请日：2010-06-30

申请人： Joseph Jump ,  Stephen Brown

发明人： Joseph Jump ,  Stephen Brown

IPC分类号： B01D61/44

CPC分类号： C12P7/46 ,  B01D61/445 ,  C07C51/487 ,  C07C59/245

摘要： The present invention relates to processes for separating and recovering the C4 dicarboxylic acid, comprising: (a) subjecting an aqueous solution comprising a salt of the C4 dicarboxylic acid to concentrating electrodialysis to concentrate the salt of the C4 dicarboxylic acid in the aqueous solution; and (b) subjecting the resulting concentrate to bipolar membrane electrodialysis to convert the salt of the C4 dicarboxylic acid into the free acid of the C4 dicarboxylic acid.

摘要翻译： 本发明涉及分离和回收C4二羧酸的方法，包括：（a）使包含C4二羧酸盐的水溶液浓缩电渗析，以将C4二羧酸的盐浓缩在水溶液中; 和（b）使所得浓缩物经双极膜电渗析将C4二羧酸的盐转化为C4二羧酸的游离酸。

公开(公告)号：US08137951B2

公开(公告)日：2012-03-20

申请号：US13084230

申请日：2011-04-11

申请人： Alan Sloma ,  Leslie Naggiar, legal representative ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Linda Sternberg, legal representative ,  Stephen Brown

发明人： Alan Sloma ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Stephen Brown

IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N15/00 ,  C12P21/00 ,  C12P9/26 ,  C12P1/00 ,  C12N9/00 ,  C12N9/24 ,  C12N9/88 ,  C07H21/04

CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12N15/52

摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

摘要翻译： 本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体 涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。 本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选地一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。 本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列。

公开(公告)号：USD642549S1

公开(公告)日：2011-08-02

申请号：US29376598

申请日：2010-10-08

申请人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

设计人： Fouad Zeinoun ,  Stephen Brown ,  Jesse Moon ,  Andrea Casati

公开(公告)号：US20110014662A1

公开(公告)日：2011-01-20

申请号：US12891548

申请日：2010-09-27

申请人： Alan Sloma ,  Leslie Naggiar ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Linda Sternberg ,  Stephen Brown

发明人： Alan Sloma ,  Leslie Naggiar ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Linda Sternberg ,  Stephen Brown

IPC分类号： C12P19/04

CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12N15/52

摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase

摘要翻译： 本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体 涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。 本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选的一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。 本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列

公开(公告)号：US07811806B2

公开(公告)日：2010-10-12

申请号：US10326185

申请日：2002-12-20

申请人： Alan Sloma ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Stephen Brown

发明人： Alan Sloma ,  Regine Behr ,  William Widner ,  Maria Tang ,  David Sternberg ,  Stephen Brown

IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N15/00 ,  C12P21/06 ,  C12P19/04 ,  C12P19/26

CPC分类号： C12P19/26 ,  C12N9/1051 ,  C12N15/52

摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

摘要翻译： 本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体 涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。 本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选地一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。 本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列。