公开(公告)号：US20110312562A1

公开(公告)日：2011-12-22

申请号：US13150016

申请日：2011-06-01

申请人： Alireza Moini ,  Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer

发明人： Alireza Moini ,  Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer

IPC分类号： C40B40/06 ,  C12M1/38 ,  C12M1/34

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the lysis section, and, a second nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the lysis section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

摘要翻译： 一种用于检测生物样品中的病原体的实验室片（LOC）装置，所述LOC装置具有用于接收样品的入口，支撑基底，用于从样品中较大成分分离病原体的透析部分，多个 所述溶剂部分在所述透析部分下游用于溶解所述病原体以在其中释放遗传物质的裂解部分，所述裂解部分与含有裂解试剂的试剂储存器流体连通，所述裂解试剂用于裂解裂解部分中的细胞，第一 在裂解部分下游的核酸扩增部分，用于扩增来自裂解部分的样品流的第一部分中遗传物质中的核酸序列，以及用于扩增核酸序列的裂解部分下游的第二核酸扩增部分 样品的第二部分中的遗传物质从裂解部分流出，其中，透析部分，裂解部分， 第一核酸扩增部分和第二核酸扩增部分都支撑在支撑基底上。

公开(公告)号：US20110312561A1

公开(公告)日：2011-12-22

申请号：US13150014

申请日：2011-06-01

申请人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

IPC分类号： C40B40/06 ,  C12M1/34 ,  G01N30/96

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A microfluidic device for detecting a target nucleic acid sequence, the microfluidic device having a probe for hybridization with the target nucleic acid sequence to form a probe-target hybrid, the probe having a fluorophore for generating fluorescence emissions in response to an excitation light, a photodiode for detecting the fluorescence emissions, a shunt transistor between the photodiode and a voltage source, and, CMOS circuitry for controlling the shunt transistor to remove carriers generated by absorption of photons of the excitation light in the photodiode.

摘要翻译： 一种用于检测靶核酸序列的微流体装置，所述微流体装置具有用于与靶核酸序列杂交以形成探针 - 靶杂交物的探针，所述探针具有响应于激发光产生荧光发射的荧光团， 用于检测荧光发射的光电二极管，在光电二极管和电压源之间的分流晶体管，以及CMOS电路，用于控制分流晶体管以去除由光电二极管中的激发光的光子吸收而产生的载流子。

公开(公告)号：US20110312560A1

公开(公告)日：2011-12-22

申请号：US13150009

申请日：2011-06-01

申请人： Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini

发明人： Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini

IPC分类号： C40B40/06 ,  C12M1/38 ,  C12M1/34

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

摘要翻译： 一种用于检测生物样品中的病原体的实验室（LOC）装置，所述LOC装置具有用于接收样品的入口，支撑底物，用于从样品中的较大组分分离病原体的透析部分，裂解 在所述透析部分的下游部分，用于裂解所述病原体以释放其中的遗传物质，所述裂解部分具有裂解室和用于在所述样品处于裂解室的情况下裂解病原体的加热器，所述裂解部分下游的第一核酸扩增部分 用于扩增遗传物质中的第一核酸序列，以及第一核酸扩增部分下游的第二核酸扩增部分，用于扩增来自第一核酸扩增部分的扩增子中的第二核酸序列，其中，透析部分， 裂解部分，第一核酸扩增部分和第二核酸扩增部分都是支持的 在支撑基底上。

公开(公告)号：US20110312545A1

公开(公告)日：2011-12-22

申请号：US13149893

申请日：2011-06-01

申请人： Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini

发明人： Kia Silverbrook ,  Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini

IPC分类号： C40B40/06 ,  C12M1/38 ,  C12M1/34

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

摘要翻译： 一种用于检测生物样品中的病原体的实验室片（LOC）装置，所述LOC装置具有用于接收样品的入口，支撑基底，用于从样品中较大成分分离病原体的透析部分，多个 所述溶剂部分在所述透析部分下游用于溶解所述病原体以在其中释放遗传物质的裂解部分，所述裂解部分与含有裂解试剂的试剂储存器流体连通，所述裂解试剂用于裂解裂解部分中的细胞，第一 用于扩增遗传物质中的第一核酸序列的裂解部分下游的核酸扩增部分和第一核酸扩增部分下游的第二核酸扩增部分，用于从第一核酸扩增扩增子中的第二核酸序列 扩增部，其中，所述透析部，所述裂解部，所述第一核酸扩增部 第二核酸扩增部全部被支撑在支撑基板上。

公开(公告)号：US20110308945A1

公开(公告)日：2011-12-22

申请号：US13150272

申请日：2011-06-01

申请人： Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini ,  Kia Silverbrook

发明人： Mehdi Azimi ,  Geoffrey Richard Facer ,  Alireza Moini ,  Kia Silverbrook

IPC分类号： G01N27/327 ,  G01N33/50

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the photosensor has a planar active surface area for receiving the light from the ECL luminophore and the electrodes are between 0.25 micron and 2 microns thick in a direction normal to the planar active surface area of the photodiodes.

摘要翻译： 一种用于检测样品中的靶核酸序列的实验室（LOC）装置，具有与靶核酸序列互补的核酸序列的探针的LOC装置用于形成探针 - 靶杂交体，以及电化学发光 （ECL）发光体，用于在ECL发光体中产生激发态的电极，其中ECL发光体发射光子，以及用于感测从ECL发光体发射的光子的光电传感器，其中，光电传感器具有平面有源表面积， 接收来自ECL发光体的光，电极在与光电二极管的平面有源表面积垂直的方向上为0.25微米至2微米厚。

公开(公告)号：US07990450B2

公开(公告)日：2011-08-02

申请号：US12497688

申请日：2009-07-05

申请人： Kia Silverbrook ,  Matthew John Underwood ,  Nicholas Damon Ridley ,  Paul Lapstun ,  Peter Charles Boyd Henderson ,  Zhenya Alexander Yourlo ,  Alireza Moini

发明人： Kia Silverbrook ,  Matthew John Underwood ,  Nicholas Damon Ridley ,  Paul Lapstun ,  Peter Charles Boyd Henderson ,  Zhenya Alexander Yourlo ,  Alireza Moini

IPC分类号： H04N3/14 ,  H04N5/335

CPC分类号： H04N5/3559 ,  H04N5/378

摘要： A photodetecting circuit is disclosed which includes a photodetector for generating a signal in response to incident light, a storage node, transfer and reset transistors and an output circuit. The storage node has first and second node terminals. The second node terminal is connected to a compensation signal during a read period of the photodetection circuit. The transfer transistor is disposed intermediate the first node terminal of the storage node and the photodetector and is for electrically connecting the first node terminal and the photodetector during an integration period upon receiving a transfer signal to a gate of the transfer transistor, allowing charge stored in the storage node to change based on the signal of the photodetector. The reset transistor has a control node for receiving a reset signal, a first terminal for receiving a reset voltage, and a second terminal electrically connected to the first node terminal, such that the reset voltage is supplied to the first node terminal when the reset signal is asserted at a gate of the reset transistor. The output circuit generates an output signal during the read period of the photodetecting circuit. The output signal is at least partially based on a voltage at the first terminal. The compensation signal is a logically negated version of the transfer signal.

摘要翻译： 公开了一种光电检测电路，其包括用于响应于入射光产生信号的光电检测器，存储节点，传输和复位晶体管以及输出电路。 存储节点具有第一和第二节点终端。 在光检测电路的读取期间，第二节点终端连接到补偿信号。 传输晶体管设置在存储节点的第一节点和光电检测器之间，并且在接收到传输晶体管的栅极的传输信号的积分周期期间电连接第一节点端子和光电检测器， 存储节点根据光电检测器的信号而改变。 复位晶体管具有用于接收复位信号的控制节点，用于接收复位电压的第一端子和电连接到第一节点端子的第二端子，使得当复位信号被复位时，复位电压被提供给第一节点端子 在复位晶体管的栅极处被断言。 输出电路在光电检测电路的读取期间产生输出信号。 输出信号至少部分地基于第一端子处的电压。 补偿信号是传输信号的逻辑上否定的版本。

公开(公告)号：US07567279B2

公开(公告)日：2009-07-28

申请号：US10778063

申请日：2004-02-17

申请人： Zhenya Alexander Yourlo ,  Paul Lapstun ,  Kia Silverbrook ,  Peter Charles Boyd Henderson ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

发明人： Zhenya Alexander Yourlo ,  Paul Lapstun ,  Kia Silverbrook ,  Peter Charles Boyd Henderson ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

IPC分类号： H04N3/14 ,  H04N5/335

CPC分类号： H04N5/3559 ,  H04N5/378

摘要： A monolithic image sensing device, including an image sensor for sensing image data; timing circuitry for generating at least one internal timing signal, the image sensor being responsive to at least one of the internal timing signals to at least commence sensing of the image data; and at least one external timing signal; at least one external pin for supplying the at least one external timing signal to at least one peripheral device.

摘要翻译： 一种单片图像感测装置，包括用于感测图像数据的图像传感器; 定时电路，用于产生至少一个内部定时信号，所述图像传感器响应于所述内部定时信号中的至少一个，以至少开始感测图像数据; 和至少一个外部定时信号; 至少一个外部引脚，用于将至少一个外部定时信号提供给至少一个外围设备。

公开(公告)号：US20050052556A1

公开(公告)日：2005-03-10

申请号：US10778061

申请日：2004-02-17

申请人： Peter Charles Boyd Henderson ,  Paul Lapstun ,  Kia Silverbrook ,  Zhenya Alexander Yourlo ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

发明人： Peter Charles Boyd Henderson ,  Paul Lapstun ,  Kia Silverbrook ,  Zhenya Alexander Yourlo ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

IPC分类号： H04N3/15 ,  H04N5/217 ,  H04N5/335

CPC分类号： H04N5/3559 ,  H04N5/378

摘要： A monolithic image sensing device including an image processor, the integrated circuit being configured to operate in a system having a host processor, the image processor being configured to receive, from the host processor, a request for access to a next available frame of image data from a framestore; in the event the frame of image data is available, sending a message to the host processor indicative of the image data's availability; and in the event the frame of image data is not available, waiting until it is available and then sending a message to the host processor indicative of the image data's availability.

摘要翻译： 一种包括图像处理器的单片图像感测装置，所述集成电路被配置为在具有主机处理器的系统中操作，所述图像处理器被配置为从所述主处理器接收对下一个可用帧的图像数据的访问请求 从一个framestore; 在图像数据帧可用的情况下，向主机处理器发送指示图像数据的可用性的消息; 并且在图像数据帧不可用的情况下，等待直到它可用，然后向主机处理器发送指示图像数据的可用性的消息。

公开(公告)号：US20050024510A1

公开(公告)日：2005-02-03

申请号：US10778056

申请日：2004-02-17

申请人： Paul Lapstun ,  Kia Silverbrook ,  Peter Charles Boyd Henderson ,  Zhenya Alexander Yourlo ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

发明人： Paul Lapstun ,  Kia Silverbrook ,  Peter Charles Boyd Henderson ,  Zhenya Alexander Yourlo ,  Alireza Moini ,  Matthew John Underwood ,  Nicholas Damon Ridley

IPC分类号： H04N3/15 ,  H04N5/217 ,  H04N5/335

CPC分类号： H04N5/3559 ,  H04N5/378

摘要： A monolithic integrated circuit including an image sensor for capturing image information; at least one analog to digital converter for converting analog signals corresponding to the image information into digital image data; and a first framestore for storing frames of the digital image data.

摘要翻译： 一种单片集成电路，包括用于捕获图像信息的图像传感器; 至少一个模数转换器，用于将对应于图像信息的模拟信号转换为数字图像数据; 以及用于存储数字图像数据的帧的第一帧存储器。

公开(公告)号：US08398940B2

公开(公告)日：2013-03-19

申请号：US13150064

申请日：2011-06-01

申请人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

发明人： Kia Silverbrook ,  Alireza Moini ,  Mehdi Azimi

IPC分类号： B01L3/00

CPC分类号： C12Q1/68 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  F16K99/003 ,  F16K99/0036 ,  Y10T137/0352 ,  Y10T137/0391 ,  Y10T137/1044 ,  Y10T137/206 ,  Y10T137/2076 ,  Y10T137/2202 ,  Y10T436/107497 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/25 ,  Y10T436/25375

摘要： A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing having a receptacle for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, a detection photosensor for exposure to the photons emitted by the ECL luminophores, control circuitry providing the electrical pulse to the electrodes, and, a universal serial bus (USB) connection such that the outer casing is configured as a USB drive for transmitting data regarding detection of the targets in the fluid to an external device, wherein during use, the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes.

摘要翻译： 用于激发被配置为检测靶核酸序列的电化学发光探针的测试模块，所述测试模块具有外壳，其具有用于接收含有靶核酸序列的流体的容器，具有用于发射光子的ECL发光体的电化学发光（ECL）探针， 在激发状态和通过谐振能量传递来猝灭来自ECL发光体的光子发射的功能部分，用于接收电脉冲以激发ECL发光体的电极，用于暴露于由ECL发光体发射的光子的检测光电传感器，控制电路提供 到电极的电脉冲，以及通用串行总线（USB）连接，使得外壳被配置为USB驱动器，用于将关于流体中的目标的检测的数据传送到外部设备，其中在使用期间，ECL 已经检测到目标核酸序列之一的探针重新配置 当由电极激发时，功能部分不会淬灭来自ECL发光体的光子发射。