公开(公告)号：US20050124789A9

公开(公告)日：2005-06-09

申请号：US09978824

申请日：2001-10-16

Applicant: Avi Ashkenazi ,  Kevin Baker ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary Gerritsen ,  Audrey Goddard ,  Paul Godowski ,  J. Christopher Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  Ivar Kljavin ,  Sophia Kuo ,  Mary Napier ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  David Shelton ,  Timothy Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William Wood

Inventor： Avi Ashkenazi ,  Kevin Baker ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary Gerritsen ,  Audrey Goddard ,  Paul Godowski ,  J. Christopher Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  Ivar Kljavin ,  Sophia Kuo ,  Mary Napier ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  David Shelton ,  Timothy Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William Wood

IPC: A61K31/17 ,  A61K38/00 ,  A61K38/17 ,  A61K38/43 ,  A61K39/395 ,  A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C07K14/435 ,  C07K14/47 ,  C07K14/52 ,  C07K14/54 ,  C07K14/705 ,  C07K16/18 ,  C07K16/28 ,  C07K16/40 ,  C12N5/06 ,  C12N9/00 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68

CPC classification number: C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4702 ,  C07K14/4703 ,  C07K14/475 ,  C07K14/52 ,  C07K14/521 ,  C07K14/54 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/70578 ,  C07K16/18 ,  C07K16/28 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/30 ,  C12N9/00 ,  C12N2799/026 ,  C12N2799/027 ,  G01N33/573 ,  G01N33/57484 ,  G01N33/68 ,  G01N2333/99 ,  G01N2800/042

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US20050079582A1

公开(公告)日：2005-04-14

申请号：US10963467

申请日：2004-10-11

Applicant: Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary Gerritsen ,  Audrey Goddard ,  Paul Godowski ,  Christopher Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  Ivar Kljavin ,  Jennie Mather ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  Timothy Stewart ,  Daniel Tumas ,  P. Williams ,  William Wood

Inventor： Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary Gerritsen ,  Audrey Goddard ,  Paul Godowski ,  Christopher Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  Ivar Kljavin ,  Jennie Mather ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  Timothy Stewart ,  Daniel Tumas ,  P. Williams ,  William Wood

IPC: C07H21/04 ,  C07K14/47 ,  C07K16/18 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/12 ,  C12N15/63

CPC classification number: C07K14/47 ,  C07K16/18 ,  C07K16/28

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US20050079513A1

公开(公告)日：2005-04-14

申请号：US10792374

申请日：2004-03-03

Applicant: Ronald Levy ,  Mark Wechser ,  Izidore Lossos ,  Robert Tibshirani ,  Ash Alizadeh ,  David Botstein

Inventor： Ronald Levy ,  Mark Wechser ,  Izidore Lossos ,  Robert Tibshirani ,  Ash Alizadeh ,  David Botstein

IPC: C12Q1/68 ,  G01N33/50 ,  G01N33/574

CPC classification number: G01N33/57426 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/16

Abstract: Methods and kits for classifying patients having diffuse large B-cell lymphoma (DLBCL) based upon expression of a plurality of genes are disclosed. Real-time quantitative RT-PCR can be used to measure expression values. Correlating expression values of the plurality of genes in a tumor sample from the patient to reference expression values obtained from DLBCL patients can stratify patients in the classification groups. The methods and kits can be used to predict overall patient survival.

Abstract translation: 公开了基于多个基因的表达来分类具有弥漫性大B细胞淋巴瘤（DLBCL）的患者的方法和试剂盒。 实时定量RT-PCR可用于测量表达值。 将来自患者的肿瘤样品中的多个基因的表达值与从DLBCL患者获得的参照表达值相关联可以对分类组中的患者进行分层。 方法和试剂盒可用于预测总体患者生存。

公开(公告)号：US20050059115A1

公开(公告)日：2005-03-17

申请号：US10943353

申请日：2004-09-17

Applicant: David Botstein ,  Audrey Goddard ,  Paul Godowski ,  J. Grimaldi ,  Austin Gurney ,  Margaret Roy ,  William Wood

Inventor： David Botstein ,  Audrey Goddard ,  Paul Godowski ,  J. Grimaldi ,  Austin Gurney ,  Margaret Roy ,  William Wood

IPC: A61K38/00 ,  A61K38/17 ,  C07H21/02 ,  C07H21/04 ,  C07K14/435 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C12N1/18 ,  C12N1/21 ,  C12N5/06 ,  C12N9/00 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/574 ,  G01N33/68

CPC classification number: C07K14/475 ,  A61K38/00 ,  C07K14/47 ,  C07K14/4703 ,  C07K14/70578 ,  C07K16/18 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02 ,  C12N2799/026 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5085 ,  G01N33/57484 ,  G01N33/68

Abstract: The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US20050043520A1

公开(公告)日：2005-02-24

申请号：US10012149

申请日：2001-12-07

Applicant: Kevin Baker ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Sherman Fong ,  Wei-Qiang Gao ,  Audrey Goddard ,  Paul Godowski ,  J. Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  Victoria Smith ,  Timothy Stewart ,  Daniel Tumas ,  Colin Watanabe ,  P. Williams ,  William Wood

Inventor： Kevin Baker ,  David Botstein ,  Luc Desnoyers ,  Dan Eaton ,  Napoleone Ferrara ,  Sherman Fong ,  Wei-Qiang Gao ,  Audrey Goddard ,  Paul Godowski ,  J. Grimaldi ,  Austin Gurney ,  Kenneth Hillan ,  James Pan ,  Nicholas Paoni ,  Margaret Roy ,  Victoria Smith ,  Timothy Stewart ,  Daniel Tumas ,  Colin Watanabe ,  P. Williams ,  William Wood

IPC: A61K38/17 ,  C07H21/04 ,  C07K14/435 ,  C07K16/40 ,  C07K16/44 ,  C12N5/06 ,  C12N9/00 ,  C12P21/02 ,  G01N33/53

CPC classification number: C07K14/47 ,  C07K14/575 ,  C07K2319/30 ,  C07K2319/40

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US06723535B2

公开(公告)日：2004-04-20

申请号：US09906700

申请日：2001-07-16

Applicant: Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan L. Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary E. Gerritsen ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin L. Gurney ,  Kenneth J. Hillan ,  Ivar J. Kljavin ,  Jennie P. Mather ,  James Pan ,  Nicholas F. Paoni ,  Margaret Ann Roy ,  Timothy A. Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William I. Wood

Inventor： Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan L. Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary E. Gerritsen ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin L. Gurney ,  Kenneth J. Hillan ,  Ivar J. Kljavin ,  Jennie P. Mather ,  James Pan ,  Nicholas F. Paoni ,  Margaret Ann Roy ,  Timothy A. Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William I. Wood

IPC: C12N1512

CPC classification number: C07K16/18

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US06664376B2

公开(公告)日：2003-12-16

申请号：US09905125

申请日：2001-07-12

Applicant: Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan L. Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary E. Gerritsen ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin L. Gurney ,  Kenneth J. Hillan ,  Ivar J. Kljavin ,  Jennie P. Mather ,  James Pan ,  Nicholas F. Paoni ,  Margaret Ann Roy ,  Timothy A. Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William I. Wood

Inventor： Avi Ashkenazi ,  David Botstein ,  Luc Desnoyers ,  Dan L. Eaton ,  Napoleone Ferrara ,  Ellen Filvaroff ,  Sherman Fong ,  Wei-Qiang Gao ,  Hanspeter Gerber ,  Mary E. Gerritsen ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin L. Gurney ,  Kenneth J. Hillan ,  Ivar J. Kljavin ,  Jennie P. Mather ,  James Pan ,  Nicholas F. Paoni ,  Margaret Ann Roy ,  Timothy A. Stewart ,  Daniel Tumas ,  P. Mickey Williams ,  William I. Wood

IPC: C07K14435

CPC classification number: C07K14/47 ,  C07K14/705 ,  C07K2319/00

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US5677153A

公开(公告)日：1997-10-14

申请号：US346333

申请日：1994-11-29

Applicant: David Botstein ,  Timothy Palzkill

Inventor： David Botstein ,  Timothy Palzkill

IPC: C12N15/10 ,  C12N15/64

CPC classification number: C12N15/102

Abstract: The invention relates to methods and mutation linkers to modify DNA, to methods for producing libraries containing a multiplicity of modified DNA, and to methods for using such libraries for screening modified proteins encoded by such DNA. The DNA targeted for modification typically encodes a polypeptide such as an enzyme. The libraries are used to determine the effect of such modification or the interaction of the modified polypeptides with a target. In preferred embodiments, the invention relates to methods for making and using libraries containing DNA encoding modified antibiotic hydrolases to screen antibiotics against one or more of the modified antibiotic hydrolases produced by such libraries. Susceptibility or lack of susceptibility of an antibiotic to neutralization provides an indication of whether wild-type antibiotic hydrolases are likely to mutate to confer resistance to the antibiotic.

Abstract translation: 本发明涉及用于修饰DNA的方法和突变接头，用于产生含有多个修饰的DNA的文库的方法，以及使用这种文库筛选由此DNA编码的修饰蛋白的方法。 靶向修饰的DNA通常编码多肽，例如酶。 文库用于确定这种修饰的作用或修饰的多肽与靶标的相互作用。 在优选的实施方案中，本发明涉及制备和使用含有编码经修饰的抗生素水解酶的DNA的文库的方法，以筛选针对一种或多种由这些文库产生的经修饰的抗生素水解酶的抗生素。 敏感性或抗生素对中和的敏感性不足提供了野生型抗生素水解酶是否可能发生变异以赋予抗生素抗性的指征。

公开(公告)号：US5108901A

公开(公告)日：1992-04-28

申请号：US384608

申请日：1989-07-24

Applicant: Stephen Anderson ,  William F. Bennett ,  David Botstein ,  Deborah L. Higgins ,  Nicholas F. Paoni ,  Mark J. Zoller

Inventor： Stephen Anderson ,  William F. Bennett ,  David Botstein ,  Deborah L. Higgins ,  Nicholas F. Paoni ,  Mark J. Zoller

IPC: C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61K38/48 ,  A61P7/02 ,  C07K14/00 ,  C07K14/47 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/64 ,  C12N9/72 ,  C12N15/58 ,  C12P21/02 ,  C12Q1/37

CPC classification number: C12N9/6456 ,  A61K38/00

Abstract: Tissue plasminogen activator (t-PA) zymogens and variants are prepared, including a fibrinolytically active variant of t-PA that has an amino acid alteration at a site within the protease domain of t-PA as compared with the corresponding wild-type t-PA, which alteration renders the variant zymogenic in the presence of plasmin-degraded fibrinogen, and/or fibrin (or plasma clot) specific, as compared to the corresponding wild-type t-PA. DNA sequences can be prepared that encode the zymogens and variants, as well as expression vectors incorporating the DNA sequences, and host cells transformed with the expression vectors. The zymogens and variants may be used in a pharmaceutical preparation to treat a vascular disease or condition or to prevent fibrin deposition or adhesion formation or reformation in mammals.

Abstract translation: 制备组织纤溶酶原激活剂（t-PA）酶原和变体，包括t-PA的纤维蛋白溶解活性变体，其在t-PA的蛋白酶结构域内的位置具有氨基酸改变，与相应的野生型t- PA，与相应的野生型t-PA相比，其改变使纤维蛋白溶酶原纤维蛋白原和/或纤维蛋白（或血浆凝块）存在下的变形酶原。 可以制备编码酶原和变体的DNA序列，以及掺入DNA序列的表达载体和用表达载体转化的宿主细胞。 酶原和变体可用于药物制剂中以治疗血管疾病或病症或预防纤维蛋白沉积或哺乳动物中的粘附形成或重新形成。